Navigation Links
DNA Yield with Oragene

H.C. Birnboim
DNA Genotek, Ottawa, Ontario, Canada

Oragene gives higher DNA yields than other oral collection methods. The median yield of DNA from Oragene samples is 110 g. In comparison, buccal swabs may yield as little as 1.9 g of DNA.


Introduction
The number of studies collecting genomic DNA from a large number of individuals is increasing rapidly. Non-invasive methods and techniques that permit selfcollection are preferred because they increase compliance rates and reduce costs. For this reason, many large-scale studies now use buccal cells from saliva as the source of DNA. The two most common methods are scraping the inside of the cheek with a brush and swishing the mouth with a mouthwash. Oragene is a DNA self-collection kit that is non-invasive and intuitive to use. The donor rinses his or her mouth with water to clear food particles and then spits 2 mL of saliva into the Oragene vial. Once the vial is closed, DNA is released and stabilized at room temperature.

The amount of DNA recovered from the oral cavity can vary widely depending on the collection method (Figure 1). This technical bulletin reports the amount of DNA obtained from saliva using the Oragene kit.


Materials and Methods
Saliva samples were obtained from 208 donors. Collection and purification of DNA was carried out according to protocols supplied with the Oragene kit. DNA yield was determined by the highly specific Fluorescence/DNase method (ref. 5). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and DNase treatment.


Results
The DNA yield of Oragene/saliva samples from 208 donors is shown in Figure 2. The median amount of DNA was 110 g. The 25th percentile was 62 g and the 75th percentile was 158 g.


Conclusions
Oragene is a non-invasive DNA self-collection kit that can be used by untrained study subjects, including children and the elderly. The median DNA yield from Oragene/saliva samples is 110 g. This is significantly higher than other oral collection methods.


References
1. Cozier, Y., Palmer, J., and Rosenberg, L. (2003) Comparison of Methods for Collection of DNA Samples by Mail in the Black Womens Health Study. AEP. 14, 117-122.

2. Harty, L., Garcia-Closas, M., Rothman, N., Reid, Y., Tucker, M., and Hartge, P. (2000) Collection of buccal cell DNA using treated cards. Cancer Epidemiology, Biomarkers & Prevention. 9, 501506.

3. Montserrat, G., Egan, K., Abruzzo, J., Newcomb, P., Titus-Ernstoff, L., Franklin, T., et al. (2001) Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiology, Biomarkers & Prevention. 10, 687-696.

4. Le Marchand, L., Lum-Jones, A., Saltzman, B., Visaya, V., Nomura, A., and Kolonel, L. (2001) Feasibility of collecting buccal cell DNA by mail in a cohort study. 10, 701-703.

5. DNA Genotek Technical Bulletin PR003 Sept 30, DNA Quantifi cation Using the Fluorescence/DNase (F/D) Assay. 2004.


back to top
'/>"/>



Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/21/2014)... , Aug. 21, 2014  Decision Resources ... Brazil and Mexico ... uptake of several newly approved agents, the anticipated ... indication and the launch of emerging biologics will ... In particular, the emerging IL-5 inhibitors will introduce ...
(Date:8/21/2014)... Cord Blood Corporation (NYSE: CO ) (the ... for the first quarter of fiscal year 2015 on ... US.  The Company will host a ... 29, 2014 to discuss its financial performance and give ... by a question and answer session.  Interested parties may ...
(Date:8/21/2014)... 21, 2014 Ontotext S4 , ... Ontotext . Now the same enterprise hardened ... structured and unstructured data is available to start-ups and ... enterprise technology. Organizations that do not have resources to ... can use S4 since there is no need for ...
(Date:8/21/2014)... August 21, 2014 On Wednesday of last ... to use Redox Signaling molecules, became available for purchase in ... , "The way that RENU 28 works is, if you ... down of the rate of cellular renewal within your body. ... increasing of that rate of cellular renewal. What RENU 28 ...
Breaking Biology Technology:Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4RENU 28 Skin Care Now Available in Australia and New Zealand 2
(Date:8/22/2014)... the 29th Annual International Papillomavirus Conference shows that ... combats three types of human papillomavirus, including the ... cancer. , When tested in several cultured human ... HPV-18 and HPV-11 cells, according to Louise T. ... the findings at the Seattle conference. The National ...
(Date:8/22/2014)... can probably remember the after-effects, including pain, swelling or ... back against the injury. When tissue in the body ... tissue regeneration. An inflammatory response acts as a protective ... to heal after injuries such as wounds and burns. ... situations in which foreign material is introduced, for example ...
(Date:8/22/2014)... tea polyphenols are strong antioxidants and can ... spinal cord neurons against oxidative stress? Jianbo ... Hospital of Liaoning Medical University, China discovered ... stress and inhibit neuronal apoptosis, indicating green ... spinal cord neurons under oxidative stress. The ...
Breaking Biology News(10 mins):Tissue regeneration using anti-inflammatory nanomolecules 2