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Agarose gels


Agarose gels: A polysaccharide gel used to measure the size of nucleic acids (in bases or base pairs). See "Gel Electrophoresis".
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Digests are analyzed on standard agarose gels, which are post-stained with ethidium bromide or thiazole orange and imaged with a Molecular Dynamics FluorImager 575.
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SOUTHERN BLOT - DNA is separated by electrophoresis (usually in agarose gels), then transferred to nitrocellulose paper or other suitable solid-phase matrix (e.g., nylon membrane), ...
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Ethidium bromide staining is commonly used to visualize RNA or DNA in agarose gels placed on UV light boxes. Proper precautions are required, because the ethidium bromide is highly mutagenic and the UV light damaging to the eyes.
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