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Alkylating agents


Alkylating agents (e.g. N-ethyl-N-nitrosourea (ENU)) These agents can mutate both replicating and non-replicating DNA. In contrast, a base analog can only mutate the DNA when the analog is incorporated in replicating the DNA.
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Alkylating agents (e.g. N-ethyl-N-nitrosourea (ENU))
Methylating agents (e.g. ethane methyl sulfonate (EMS))
Polycyclic hydrocarbons (e.g. benzpyrenes found in internal combustion engine exhaust)
DNA intercalating agents (e.g. ethidium bromide) ...
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Alkylating agents (EMS, MNNG, MNU) are used when substitutions are desired. Small insertions arise from the use of intercalating agents, such as acridines, ICR191, and proflavine, agents that slip between the base pairs.
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