'Nanodrop' test tubes created with a flip o...(the droplet. In one set of fluorescence ...)

Date:4/15/2008

the droplet. In one set of fluorescence experiments, explains Goldner, The molecules seem unperturbed by their confinementthey do not stick to the walls or leave the containerimportant facts to know for doing nanochemistry or single-molecule biophysics. Similar to a previous work (see Micro-boxes of Water Used to Study Single Molecules, Tech Beat July 20, 2006), researchers also demonstrated that single fluorescent protein molecules could be detected inside the droplets.

Fluorescence can reveal the number of molecules within the nanodroplet and can show the motion or structural changes of the confined molecule or molecules, allowing researchers to study how two or more proteins interact. By using only a few molecules and tiny amounts of reagents, the technique also minimizes the need for expensive or toxic chemicals.

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Contact: Evelyn Brown evelyn.brown@nist.gov 301-975-5661 National Institute of Standards and Technology (NIST) Source:Eurekalert

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