HOME >> BIOLOGY >> NEWS
A subtle tool to study mankind's diseases

rocess works a bit like a "find-and-replace" command in your word processor. Imagine you have typed "Stephen Q. Gould" everywhere, and suddenly discover that the middle initial should be "J". The computer can be told to look for "Stephen" and "Gould" and replace what comes between them. In the same way, recombinases find recognizable sequences of DNA to the left and right of a target and replace what comes in between with the new sequence.

Homologous recombination was known to occur in bacteria, but it hadn't been possible to use it to engineer DNA, as was the case in yeast and mouse stem cells. Stewart?s team decided to try to find a strain that could do it. "We ordered as many types of E. coli as we could, looking for defects in the way they repair their DNA," he says. "After five months of work, Youming Zhang, a postdoc in the lab, found the strain."

The group quickly identified the bacterial factors involved and turned them into a new tool called Red/ET recombination that is now being adopted by biologists all over the world. It's one of the mainstays of Gene Bridges GmbH, a company that Stewart and his colleagues founded with EMBL to develop the commercial implications of the breakthrough.

"We have been pushing it to work with larger and larger bits of DNA," Testa says, "and our latest project has been to engineer an entire artificial chromosome in bacteria. We've constructed a large, complex 'cassette' that we've now inserted into a mouse in place of its normal gene."

The gene that they chose is called mixed-lineage leukemia (Mll), and is known to become defective in childhood leukemias in humans. By inserting the artificial version into the mouse, researchers hope to understand how the defects lead to disease. "There are many things that can go wrong in this gene," Testa says, "and we wanted to construct a version of it that would allow us to test as many aspects of the problem as possible."

The artificial Mll that
'"/>

Contact: Russ Hodge
hodge@embl.de
49-6221-387-252
European Molecular Biology Laboratory
24-Mar-2003


Page: 1 2 3

Related biology news :

1. New method is first to mimic subtle genetic changes
2. Student science contest participation influences study, career choices, alumni say
3. New study shows hope for treating inhalant abuse
4. International study findings link acne-like rash to effectiveness of new targeted cancer treatment
5. Cigarette smoke causes breaks in DNA and defects to a cells chromosomes, Pitt study finds
6. New study indicates arsenic could be suitable as first-line treatment in type of leukaemia
7. Phase II trials of second-generation antisense cancer drug planned following successful early study
8. Preclinical safety study shows adipose-derived stem cells improve heart function after heart attack
9. Indiana University, EPA to study airborne PCBs
10. K-State, other universities to study how climate affects plant evolution
11. USC study links historical increases in life span to lower childhood exposure to infection

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/6/2017)...  Delta ID Inc., a leader in consumer-grade iris ... at CES® 2017. Delta ID has collaborated with Gentex ... use of iris scanning as a secure, reliable and ... a car, and as a way to elevate the ... Delta ID and Gentex will demonstrate (booth #7326 LVCC) ...
(Date:1/3/2017)... VEGAS , Jan. 3, 2017 Onitor, ... the introduction of Onitor Track, an innovative biometric data-driven ... men, showcasing this month at the 2017 Consumer Electronics ... In the U.S., the World Health ... more than two-thirds of adults who are overweight or ...
(Date:12/20/2016)... Dec. 20, 2016 The rising popularity ... and leasing is stoking significant interest in keyless ... technology, Bluetooth low energy (BLE), biometrics and near-field ... next wave of wireless technologies in the automotive ... to advanced access systems opens the market to ...
Breaking Biology News(10 mins):
(Date:1/18/2017)... , Jan. 18, 2017  HUYA Bioscience International, (HUYA), ... China,s pharmaceutical innovations, announced today a ... Innovation and Investment Company (referred to as CAS Innovation). ... innovations discovered by leading scientists at CAS to meet ... HUYA is the first company to have recognized ...
(Date:1/17/2017)... West Point, IA (PRWEB) , ... January 17, ... ... approval from China for Balance™ GT soybeans. The new Balance™ GT Soybean Performance ... tolerance to glyphosate and isoxaflutole, the active ingredient in the new Balance® Bean ...
(Date:1/17/2017)... Mass. , Jan. 17, 2017   Pulmatrix, ... stage biopharmaceutical company developing innovative inhaled therapies to address ... for treating fungal infections in the lungs of CF ... Disease Product" (QIDP) by the U.S. Food & Drug ... which is designed to speed the development of novel ...
(Date:1/17/2017)... UK (PRWEB) , ... January ... ... presented by surgeons at the 2016 annual meeting of the North American ... clinical outcomes, for patients in the majority of cases, when PEEK-OPTIMA™ HA ...
Breaking Biology Technology:
Cached News: