HOME >> BIOLOGY >> NEWS
A subtle tool to study mankind's diseases

rocess works a bit like a "find-and-replace" command in your word processor. Imagine you have typed "Stephen Q. Gould" everywhere, and suddenly discover that the middle initial should be "J". The computer can be told to look for "Stephen" and "Gould" and replace what comes between them. In the same way, recombinases find recognizable sequences of DNA to the left and right of a target and replace what comes in between with the new sequence.

Homologous recombination was known to occur in bacteria, but it hadn't been possible to use it to engineer DNA, as was the case in yeast and mouse stem cells. Stewart?s team decided to try to find a strain that could do it. "We ordered as many types of E. coli as we could, looking for defects in the way they repair their DNA," he says. "After five months of work, Youming Zhang, a postdoc in the lab, found the strain."

The group quickly identified the bacterial factors involved and turned them into a new tool called Red/ET recombination that is now being adopted by biologists all over the world. It's one of the mainstays of Gene Bridges GmbH, a company that Stewart and his colleagues founded with EMBL to develop the commercial implications of the breakthrough.

"We have been pushing it to work with larger and larger bits of DNA," Testa says, "and our latest project has been to engineer an entire artificial chromosome in bacteria. We've constructed a large, complex 'cassette' that we've now inserted into a mouse in place of its normal gene."

The gene that they chose is called mixed-lineage leukemia (Mll), and is known to become defective in childhood leukemias in humans. By inserting the artificial version into the mouse, researchers hope to understand how the defects lead to disease. "There are many things that can go wrong in this gene," Testa says, "and we wanted to construct a version of it that would allow us to test as many aspects of the problem as possible."

The artificial Mll that
'"/>

Contact: Russ Hodge
hodge@embl.de
49-6221-387-252
European Molecular Biology Laboratory
24-Mar-2003


Page: 1 2 3

Related biology news :

1. New method is first to mimic subtle genetic changes
2. Student science contest participation influences study, career choices, alumni say
3. New study shows hope for treating inhalant abuse
4. International study findings link acne-like rash to effectiveness of new targeted cancer treatment
5. Cigarette smoke causes breaks in DNA and defects to a cells chromosomes, Pitt study finds
6. New study indicates arsenic could be suitable as first-line treatment in type of leukaemia
7. Phase II trials of second-generation antisense cancer drug planned following successful early study
8. Preclinical safety study shows adipose-derived stem cells improve heart function after heart attack
9. Indiana University, EPA to study airborne PCBs
10. K-State, other universities to study how climate affects plant evolution
11. USC study links historical increases in life span to lower childhood exposure to infection

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: subtle tool study mankind diseases

(Date:10/15/2014)... be awarded the 44th Rosenstiel Award for Distinguished Work ... research exploring the mechanisms of genomic instability and its ... is the second alumnus to win the Rosenstiel Award; ... 2003. , Alt is the Charles A. Janeway Professor ... School and an investigator at the Howard Hughes Medical ...
(Date:10/14/2014)... shows SIRT6—a protein known to inhibit the growth ... skin cancers by turning on an enzyme that increases ... Previously considered protective, SIRT6 is part of a family ... stability and prevent some of the genetic flaws associated ... lead to cancer. This study, in the journal,s October ...
(Date:10/14/2014)... questions is how to produce enough food to feed ... and Agriculture Organization of the United Nations predicts that ... years to feed a growing global population, and plants ... food production. Plants—grains, cereals, fruits, vegetables, and more—feed ... research must tap into our knowledge of how plants ...
Breaking Biology News(10 mins):Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
(Date:10/20/2014)... 2014 Mapp Biopharmaceutical,s valiant effort to ... to fight the Ebola outbreak will make the ... production of pharmaceuticals can be, according to Kalorama ... while some may be taken aback by the ... industry knowledge are well aware of the complex ...
(Date:10/20/2014)... , October 20, 2014 ... gastrointestinal (GI) disorders and the delivery of care has ... diseases and worrying inequalities in the provision of healthcare ... which was commissioned by United European Gastroenterology (UEG), have ... political and public awareness of the burden of GI ...
(Date:10/20/2014)... Earle Martin , Chief Executive ... Ellen Teplitzky, an experienced attorney specializing in business ... has joined the firm as Director of Legal ... NDA Partners provides legal services, including research, ... top law firms and their clients in cases ...
(Date:10/19/2014)... 19, 2014 NextCODE Health, which enables clinicians and ... real time, today announced the launch of its new genomic ... at the American Society of Human Genetics (ASHG) annual meeting ... sign up and apply for free beta access, visit ... benefits can be viewed here . The ...
Breaking Biology Technology:Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 2Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 3Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 4Major Survey Reveals Changing Trends and Inequalities in Healthcare Provision for Gastrointestinal Disorders Across Europe 5NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3
Cached News: