HOME >> BIOLOGY >> NEWS
A subtle tool to study mankind's diseases

rocess works a bit like a "find-and-replace" command in your word processor. Imagine you have typed "Stephen Q. Gould" everywhere, and suddenly discover that the middle initial should be "J". The computer can be told to look for "Stephen" and "Gould" and replace what comes between them. In the same way, recombinases find recognizable sequences of DNA to the left and right of a target and replace what comes in between with the new sequence.

Homologous recombination was known to occur in bacteria, but it hadn't been possible to use it to engineer DNA, as was the case in yeast and mouse stem cells. Stewart?s team decided to try to find a strain that could do it. "We ordered as many types of E. coli as we could, looking for defects in the way they repair their DNA," he says. "After five months of work, Youming Zhang, a postdoc in the lab, found the strain."

The group quickly identified the bacterial factors involved and turned them into a new tool called Red/ET recombination that is now being adopted by biologists all over the world. It's one of the mainstays of Gene Bridges GmbH, a company that Stewart and his colleagues founded with EMBL to develop the commercial implications of the breakthrough.

"We have been pushing it to work with larger and larger bits of DNA," Testa says, "and our latest project has been to engineer an entire artificial chromosome in bacteria. We've constructed a large, complex 'cassette' that we've now inserted into a mouse in place of its normal gene."

The gene that they chose is called mixed-lineage leukemia (Mll), and is known to become defective in childhood leukemias in humans. By inserting the artificial version into the mouse, researchers hope to understand how the defects lead to disease. "There are many things that can go wrong in this gene," Testa says, "and we wanted to construct a version of it that would allow us to test as many aspects of the problem as possible."

The artificial Mll that
'"/>

Contact: Russ Hodge
hodge@embl.de
49-6221-387-252
European Molecular Biology Laboratory
24-Mar-2003


Page: 1 2 3

Related biology news :

1. New method is first to mimic subtle genetic changes
2. Student science contest participation influences study, career choices, alumni say
3. New study shows hope for treating inhalant abuse
4. International study findings link acne-like rash to effectiveness of new targeted cancer treatment
5. Cigarette smoke causes breaks in DNA and defects to a cells chromosomes, Pitt study finds
6. New study indicates arsenic could be suitable as first-line treatment in type of leukaemia
7. Phase II trials of second-generation antisense cancer drug planned following successful early study
8. Preclinical safety study shows adipose-derived stem cells improve heart function after heart attack
9. Indiana University, EPA to study airborne PCBs
10. K-State, other universities to study how climate affects plant evolution
11. USC study links historical increases in life span to lower childhood exposure to infection

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: subtle tool study mankind diseases

(Date:1/22/2015)... 2015 Infinisource has launched its new NXG series of ... G2 sets a higher standard for collecting attendance and labor ... With plug-and-play installation, touch screen interface and seamless connection to ... time collection solution for the small to mid-size employer. ...
(Date:12/22/2014)... DENVER , Dec. 22, 2014  The 2014 Holiday ... industry as Acuity Market Intelligence reports that the long ... Acuity forecasts that intensifying demand for smart phones, tablets, ... a global market of 2.5 billion users with nearly ...
(Date:12/19/2014)... , Dec. 18, 2014   LaunchKey , the ... the post-password and Internet of Things era, today announced ... The venture round was led by Metamorphic Ventures with ... Venture Partners, VegasTechFund, and others.  LaunchKey has raised $4 ...
Breaking Biology News(10 mins):Infinisource's NXG series sets new time clock standard 2First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3
(Date:1/22/2015)... Boston, MA (PRWEB) January 22, 2015 ... systems, is pleased to announce an exciting product from a ... of motion analysis solutions over the past 15 years, with ... industries. Most recently, their 3D IMU system (inertial measurement unit), ...
(Date:1/22/2015)... Diagenode, Inc., a leading global ... and complete solutions for epigenetics research, recently launched ... the need for manual processing. The new ChIPettor ... of histones or transcription factors and a semi-automated ...
(Date:1/22/2015)... , Jan. 22, 2015   GenoSpace , a precision medicine ... to enable the broad use of genomic, imaging and other ... appointment of Michelle Munson , CEO of Aspera, an ... Logo - http://photos.prnewswire.com/prnh/20150122/170713 "We ...
(Date:12/24/2014)... DALLAS and NEW YORK , ... a biopharmaceutical company advancing patient care in critical areas, announced ... of common stock, and warrants to purchase up to an ... of $4.00 per share and $.01 per warrant.  The warrants ...
Breaking Biology Technology:3D Motion Capture Just Got Easy 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2GenoSpace Expands Board with Appointment of Michelle Munson 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3
Cached News: