"Gene amplification is a common and critically important genetic defect in cancer cells, and chromosomal band 11q13 appears to be one of the most frequently amplified chromosomal regions. Yet, until now, a comprehensive physical map of the band has not been published," said corresponding author Susanne M. Gollin, Ph.D., associate professor, department of human genetics, Graduate School of Public Health and director, Cytogenetics Facility, University of Pittsburgh Cancer Institute (UPCI).
"Our work represents the first time the structure of the 11q13 amplicon has been mapped so finely, enabling us to identify a new gene in this segment that is both amplified and overexpressed in oral cancer cells."
Amplification of band 11q13 appears to be a relatively common event in the development of oral and head and neck cancers, and it is usually associated with a poor prognosis. In addition to oral and head and neck cancers, amplification of the 11q13 region has been reported in breast, lung, bladder and esophageal cancers.
In this study, investigators examined 30 oral squamous cell carcinoma cell lines developed from tumors removed from patients who had not been treated previously. A new technique called quantitative microsatellite analysis (QuMA) was used to map the 11q13 amplicon, which houses about 10 genes. Of the cell lines examined, 16 (63 percent) had ampl
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Contact: Kathryn Duda
DudaK@upmc.edu
412-624-2607
University of Pittsburgh Medical Center
5-Aug-2002