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Animal Studies Indicate Aging Brain Responsive To Estrogen

nal death that leads to the cognitive decline.

"Estrogen replacement therapy might 'shore up' the synaptic connections of neurons affected by the disease," said Einstein.

Earlier behavioral findings from Williams' lab had already shown that older female rats' spatial memory is improved by estrogens administered in a cyclic fashion. These findings led to the cellular studies in Einstein's lab that now suggest a mechanism for this improvement in memory.

In the study, the team used aging female rats that had their ovaries removed and, thus, were making no estrogen. The researchers divided the rats into three treatment groups -- those receiving no estrogens, long-term chronic estrogen doses, or a single acute dose of estrogen.

After the treatments, they performed microscopic studies that allowed them to determine how the treatments affected neurons called granule cells in the rats' hippocampus, a brain region involved in spatial memory. The scientists' study of single neurons revealed that the rats exposed to acute estrogen treatment had 40 percent more connective elements, called "dendritic spines," on these neurons than did aged female rats with no estrogen replacement or those exposed to long-term chronic estrogens. Dendritic spines may be the basis for the brain's ability to develop new memories, the scientists said.

"These studies suggest that the hippocampus of the female rat responds best if it is exposed to estrogen in cyclical fashion, as is the case in young adult female rats," Williams said in an interview. "Giving aging females estrogen in a constant fashion was no better for their brain than giving no estrogen at all.

"We're not yet sure whether this is also true in males, and one of our next steps is to determine if neurons in aged males are responsive to estrogens as well. They may or may not be since, paradoxically, males have estrogens in their brains naturally throughout life, because testosterone i
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Contact: Dennis Meredith
meredithd@mail01.adm.duke.edu
919-681-8054
Duke University
20-Nov-1996


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