HOME >> BIOLOGY >> NEWS
Basic RNA enzyme research promises single-molecule biosensors

ANN ARBOR, Mich.---Research aimed at teasing apart the workings of RNA enzymes eventually may lead to ways of monitoring fat metabolism and might even assist in the search for signs of life on Mars, according to University of Michigan researcher Nils Walter.

His latest work was published online in the Proceedings of the National Academy of Sciences June 24.

Walter and associates at U-M and colleague Xiaowei Zhuang and associates at Harvard University, use techniques that allow them to study single molecules of RNA enzymes, also known as ribozymes. Like the more familiar protein enzymes, RNA enzymes accelerate chemical reactions inside cells. Researchers want to learn how changes in ribozyme molecules affect their activity, both to better understand how evolution has shaped ribozymes to carry out their duties and to find ways of manipulating them for useful purposes.

In the recent research, Walter's group combined a technique called single-molecule fluorescence resonance energy transfer (FRET) with mathematical simulations to study a ribozyme involved in the replication of a tobacco-infecting virus. Just as a protein enzyme is not a static structure, a ribozyme also changes shape, cycling back and forth between its compact, catalytically active form and its inactive, extended form. Single-molecule FRET allowed the researchers to directly observe and measure how quickly the ribozyme switched forms and how these rates changed when various parts of the molecule were altered.

With the addition of mathematical simulations, the researchers also could investigate how changing parts of the ribozyme molecule affected its ability to catalyze chemical reactions. They were surprised to find that modifications they made anywhere on the molecule---even far from the site where the chemical reaction occurs---affected the rate of catalysis.

That's much like what is known to happen in protein enzymes, but until now there was no evidence that
'"/>

Contact: Nancy Ross Flanigan
rossflan@umich.edu
734-647-1853
University of Michigan
29-Jun-2004


Page: 1 2 3

Related biology news :

1. Basic motion measuring technology may be alternative to more expensive testing procedures
2. Basic Research and Higher Education Program Conference breaks new ground
3. Clay M. Armstrong, MD, wins 1999 Albert Lasker Basic Medical Research Award
4. Cystic Fibrosis Conference Will Showcase Latest From Clinical Care To Basic Science
5. Butterfly Wings, Beetle Horns Teach Biologists Basic Lesson In Developments Laws
6. Overfeeding Normal Infant Rats Affects Three Generations: Permanently Changes Basic Metabolism, Interferes With Reproduction
7. Ten Years of Progress For Superfund Basic Research Program
8. Anthrax enzyme images reveal secrets of antibiotic resistance, suggest new drug design
9. First glimpse of DNA binding to viral enzyme
10. Two enzymes key to calorie-burning, Brown research shows
11. Argonne scientists determine structure of staph, anthrax enzyme

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Basic RNA enzyme research promises single molecule biosensors

(Date:8/19/2014)... release is available in German . ... inherently intermittent energy sources. If a large amount of electricity ... future, excess energy will have to be stored during productive ... existing storage capacities are far from adequate for the purpose. ... One important focus lies on battery systems that used to ...
(Date:8/19/2014)... a lemur. It,s not an African Bush Baby or even ... and downright "cool" primate from Southeast Asia. , "It,s really ... Rafe Brown, curator-in-charge at the University of Kansas, Biodiversity Institute. ... furry body; a long tail with a furry tuft at ... look a bit like the disks on the digits of ...
(Date:8/19/2014)... retail seafood counters in 10 different states show ... to unexpectedly high levels of mercury, a harmful ... fish of the same species, but from a ... according to new research by University of Hawai,i ... is essential to allow consumers to choose sustainable ...
Breaking Biology News(10 mins):Asian inventions dominate energy storage systems 2Asian inventions dominate energy storage systems 3Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 2Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 3Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3
(Date:8/20/2014)... have it that American Indians largely were wiped out ... and tuberculosis brought to the New World by European ... million people lived in the Americas shortly before Europeans ... by European diseases., But new research led by anthropological ... Krause of the University of Tubingen in Germany indicates ...
(Date:8/20/2014)... , Aug. 20, 2014 Neurotrope, ... host a conference call to provide an update ... p.m. Eastern daylight timeDial-in numbers:(888) 505-4369 (U.S. and ... Live web cast: www.neurotropebioscience.com , under "Investor Relations"The ... after completion through September 2, 2014 at (888) ...
(Date:8/20/2014)... , Aug. 20, 2014  Decision Resources Group finds that ... Russia , India and ... billion in 2013, roughly equal to the size of ... the BRIC market will experience much faster growth as a result ... population. Other key findings from Decision Resources Group,s coverage ...
(Date:8/20/2014)... The 6th Annual Arrowhead Personalized and ... 29-30 in San Francisco, CA. This year’s conference brings ... and clinicians focused on addressing and solving the significant ... twelve years since the sequencing of the human genome, ... pharmacogenetic information in the label and molecular diagnostics have ...
Breaking Biology Technology:Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3Neurotrope To Host Conference Call 2BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 2BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 36th Annual Personalized and Precision Medicine Conference Bringing Together Thought Leaders to Discuss Clinical Implementation, Dx Reimbursement, Big Data, and Sequencing 2
Cached News: