HOME >> BIOLOGY >> NEWS
Blood stem cells carry targeted genes

Researchers at the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins have genetically altered human blood stem cells to selectively activate genes in developing immune cells. Results of the research in mice, published in the January 15 issue of Blood, shows it's possible to transfer genes into stem cells and activate the immune system to fight cancer and enhance transplantation.

"Blood stem cells represent an important target for the treatment of a variety of blood and immune disorders, so our ability to engineer them to selectively stimulate immune responses opens up new possibilities for gene therapy," says Linzhao Cheng, Ph.D., assistant professor of oncology at the Johns Hopkins Kimmel Cancer Center and director of the study.

Using a gene known to produce a fluorescent protein, scientists transferred it into human adult and cord blood stem cells and injected the cells into immune-compromised mice. The gene transfer into the stem cells was accomplished via a lentivirus, genetically engineered to be safe, with coded instructions for the gene to turn on in a specific type of cell.

Since blood stem cells differentiate and develop into all blood and immune system cells, all descendants of the stem cells had the fluorescent protein gene. However, the gene turns on only when the stem cell developed into one type of immune cell, called an antigen-presenting cell (APC). APCs play a central role in controlling immune system responses.

"The ability to deliver a gene in a stem cell and then have it expressed in one specific type of cell should provide a new way to achieve targeted gene therapy," says Cheng.

Six mice were transplanted with the fluorescent gene made specific for APCs. After 10 weeks, all produced the fluorescent protein in an average of 56 percent of the transplanted cells, and exclusively in APCs. Five control mice were transplanted with the fluorescent gene made universal for all cells and pr
'"/>

Contact: Amy Heaps
heapsam@jhmi.edu
410-955-1287
Johns Hopkins Medical Institutions
8-Jan-2002


Page: 1 2

Related biology news :

1. Blood pressure hormone may inhibit growth of lung cancer
2. Blood tests identify patients on dialysis at high risk for death from cardiovascular disease
3. Blood banking systems improving in China, more progress needed
4. Blood proteins tied to inflammation
5. Bloodworms way with copper likely provides paradigm for new materials
6. Blood markers may reflect newborns potential of contracting HIV
7. Blood vessels trigger development of the pancreas
8. Blood vessels found to signal chain of destruction in bone diseases
9. Blood markers associated with autism and mental retardation
10. Blood test for diagnosing schizophrenia
11. Blood pressure-lowering DASH diet also reduces homocysteine

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/7/2020)... ... 2020 , ... Alucio™, a fast-growing provider of cloud-based software ... Jessica Wong have joined the company’s leadership team as Vice President of Engineering, ... development efforts for Alucio’s flagship product, Beacon, an innovative cloud-based content management and ...
(Date:7/31/2020)... Md. (PRWEB) , ... July 30, 2020 , ... ... Innovation Research (SBIR) Phase II contract awarded by the Joint Science & Technology ... of this project is to develop, optimize, and scale-up a highly efficient mammalian ...
(Date:7/18/2020)... ... July 17, 2020 , ... Commercial launch readiness is a critical stage ... a COVID cure or vaccine, the global economic downturn will only increase price pressures ... going away and capturing full value from every product launch is critical. However, history ...
Breaking Biology News(10 mins):
(Date:8/7/2020)... ... August 06, 2020 , ... VGXI, a highly regarded ... on the purchase of greenfield for a new, expanded manufacturing facility. The site ... acres in the initial acquisition, with an option to purchase an additional 21 ...
(Date:7/31/2020)... ... 2020 , ... R3 Stem Cell International is now offering patients the opportunity ... cells total, patients may choose which extremities they would like treated. , Several studies ... Disord. 2016). At R3 International, umbilical cord tissue is obtained from a lab with ...
(Date:7/31/2020)... ... 29, 2020 , ... Anomet Products has introduced new custom ... use with CRM, neurostimulation, vascular, and related devices. , Anomet Medical Clad ... performance, and cost criteria; especially where solid wire is limited. Typical configurations for ...
(Date:7/31/2020)... ... July 29, 2020 , ... ... top radiation centers in 16 countries, has reached its 20th anniversary of worldwide ... in routine use at top universities including University of Pennsylvania, University of Michigan, ...
Breaking Biology Technology:
Cached News: