HOME >> BIOLOGY >> NEWS
Busy sequencing technique saves money and time

A computer scientist at Washington University in St. Louis has developed a novel technique to extract more DNA from a single sequence reaction than is normally possible, reducing both cost and time of the sequencing process.

Michael R. Brent, Ph.D., associate professor of computer science, has applied software developed in his Washington University laboratory that sorts through the maize of genetic information and finds predicted sequences.

"Normally, you get one 600 to 700 base pair sequence in a reaction, but under certain conditions, we've figured out how to get more than one sequence out of a single sequencing reaction," said Brent. "In most cases, people would throw out a reaction with more than one sequence but we've developed software that allows us to sort out the mess and figure out the different sequences."

Writing in the April issue of Genome Research, Brent and collaborators at Baylor College of Medicine, led by Richard A. Gibbs, Ph.D., director of Baylor's Human Genome Sequencing Center, discuss related techniques in genome analysis, while noting that the recent publication of a third mammalian genome, the brown rat, suggests a new approach to genome annotation is needed. Sequencing genomes has proven to be so labor-intensive and expensive that researchers fear little headway will be made in future genome analyses. Thus, the need for automated analysis.

The researchers describe their method of predicting genes in the brown rat using Brent's TWINSCAN software, which predicts the existence of genes by looking at two genomes in parallel and homing in on statistical patterns in the individual DNA sequences of each genome. The recently completed sequencing of the brown rat genome was conducted primarily using another program called Ensembl.

Brent and his collaborators tested 444 TWINSCAN-predicted rat genes that showed significant homology, or correspondence, to known human genes implicated in disease. Ensembl and other
'"/>

Contact: Tony Fitzpatrick
tony_fitzpatrick@wustl.edu
314-935-5272
Washington University in St. Louis
10-May-2004


Page: 1 2

Related biology news :

1. Washington University in St. Louis plays key role in sequencing moss genome
2. JGI announces community sequencing program portfolio
3. NHGRI adds 18 organisms to sequencing pipeline
4. Kangaroo hops in line for genome sequencing
5. Laboratory rat gene sequencing completed
6. Marsupial among model organisms next in line for sequencing
7. Scientists report first sequencing of environmental genome
8. JGI launches community sequencing program
9. New technology will speed genome sequencing
10. NHGRI selects sequencing centers
11. K-State scientists beetle chosen for national genome sequencing project

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:11/3/2018)... ... November 01, 2018 , ... ... Guidance, New Challenges and New Opportunities, **Presented by FDAnews**, Dec. 6, 2018 – ... , Early bird pricing for Emerging Trends in Medical Device Regulation and ...
(Date:11/3/2018)... Colo. (PRWEB) , ... November 01, 2018 , ... As ... Union (EU) continue to alter the landscape of quality systems for manufacturers worldwide. To ... medical device makers must act on now. , “While 2020 may sound far off, ...
(Date:10/31/2018)... ... October 30, 2018 , ... ODSC West 2018, the latest in ... 31 through Nov. 3. The event offers more than 200 training sessions and workshops ... companies striving to advance the state of the art. , ODSC West 2018 ...
Breaking Biology News(10 mins):
(Date:11/5/2018)... ... November 05, 2018 , ... ... to 2Blades’ Transcription Activator Like (TAL) effector code technology for applications in advancing ... of the technology in plants. Since its discovery in 2009, the efficacy of TAL ...
(Date:10/31/2018)... ... October 30, 2018 , ... Rooam , the premier payment platform ... this winter. After its acquisition of the Tally payment app, the leading payment platform ... tips, Rooam will grow its market share and increase the number of restaurant and ...
(Date:10/31/2018)... ... 30, 2018 , ... The assessment of cell health and ... experimental biology. Reproducibility of cell-based assays is one of the key points when ... type, there are many more factors to be considered to decrease data variability. ...
(Date:10/25/2018)... ... , ... To prepare for radiological and nuclear emergencies, the U.S. Department of ... to repurpose its commercial Silverlon ® technology for radiation injuries of the ... been awarded a three-year, $8.4 million contract with the Biomedical Advanced Research and Development ...
Breaking Biology Technology:
Cached News: