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Busy sequencing technique saves money and time

techniques that use protein-to-genome mapping missed these genes. Brent and his collaborators verified the existence of 59 percent of their predicted genes.

"We showed that we can do this efficiently with a reasonable fraction of the genes that TWINSCAN predicts and that you can actually produce a gene structure with the method," Brent said. "These predictions are a viable springboard for doing experiments. When you start with a prediction you'll get an experimental result pretty frequently. We believe it's a good way to complete the annotation of a genome."

The approach stands traditional genome annotation on its head because it starts with a computer analysis of genome data, using that as a hypothesis and drawing experiments from the hypothesis.

"Currently, experimental sequencing of both genomes and gene products is followed by computational analysis of the resulting sequences," said Brent. "It's a one way street. We want to integrate computational and experimental genomics, so that the parts of the process talk to each other."


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Contact: Tony Fitzpatrick
tony_fitzpatrick@wustl.edu
314-935-5272
Washington University in St. Louis
10-May-2004


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