Changing colors in mice

As published in Genes & Development, researchers from the University of Virginia have developed a new and powerful transgenic mouse model system. This system allows scientists to introduce a foreign gene into the mouse and turn this gene on and off at will through a simple dietary change. The paper details the careful genetic manipulation behind this work and the eye-catching results mice that change color when a special supplement is added to their drinking water, and revert back to their original color once the supplement is removed!

Dr. Scrable and colleagues genetically engineered the bacterial lac operon for use as an inducible gene regulatory system in the mouse. The lac regulatory system in the mouse provides tight, reversible control of specific gene expression a tool that will be of wide interest to those who model human disease and development.

The lac operon in E. coli consists of a set of regulatory elements and structural genes whose products enable the bacterial cell to metabolize lactose. In the absence of lactose, lac structural genes are not transcribed because the lac repressor protein is bound to the lac operator, thereby preventing RNA polymerase from initiating transcription. When the lactose analog IPTG is present, the lac repressor protein binds IPTG and undergoes a conformational change that decreases its affinity for the lac operator and allows for transcription to occur. Dr. Scrable and colleagues have spent the past four years adapting this bacterial operon for use as a gene regulatory system in the mouse.

Dr. Scrable and colleagues manipulated the DNA sequence and gene structure of the lac repressor protein so that it is expressed ubiquitously in the mouse. By strategically integrating lac operator sequences within the promoter of a reporter gene, tyrosinase, Dr. Scrable and colleagues generated a mouse strain that expresses tyrosinase only in the presence of IPTG. As tyrosinase encodes an enzyme necessary

Contact: Nora Poppito
Cold Spring Harbor Laboratory

Page: 1 2

Related biology news :

1. Changing one gene launches new fly species
2. Changing the face of biology
3. Changing focus of traditional hospital rounds improves patient care
4. Changing global nitrogen cycle impacting human health, says Colorado University-led study
5. Changing fish body shapes give clues to environmental factors
6. Relationships in a Changing World -- call for papers
7. Changing childrens diets today could reduce bone problems 70 years from now
8. If You Cant Stop Polluting, Try Changing The Weather Instead
9. USGS Finds Sea Otters At Risk From Killer Whales In A Changing Ocean
10. Corn Rootworm Changing Behavior, Posing New Threat To Crops
11. Changing Continental Runoff Patterns Could Change Ocean Circulation

Post Your Comments:

(Date:3/27/2021)... ... March 25, 2021 , ... Phlexglobal announced ... Planet Group, has selected Phlexglobal and its innovative regulatory SaaS software, PhlexSubmission, as ... a comprehensive review of five regulatory software companies, with the review team including ...
(Date:3/27/2021)... ... 25, 2021 , ... The 2021 Virtual Conference on Clinical Trial Supply-Europe ... More and more, clinical trial supply conferences are featuring speakers and forums that ... Asymmetrex’s founder and CEO, James L. Sherley, M.D., Ph.D., presented a talk ...
(Date:3/23/2021)... ... March 23, 2021 , ... G-CON Manufacturing (G-CON), the ... by Matica Biotechnology (Matica Bio), a contract development and manufacturing organization (CDMO) specializing ... the cleanroom build out for its new GMP production facility in College Station, ...
Breaking Biology News(10 mins):
(Date:3/27/2021)... ... March 24, 2021 , ... ... Life Sciences and Healthcare firms of all sizes, adds depth to its team ... specialist. Pardillo, who earned his doctorate in computational chemistry from Florida International University ...
(Date:3/27/2021)... ... March 24, 2021 , ... ABI Wellness, ... and reporting approach designed under CEO Mark Watson, today announced a webinar dedicated ... featuring guest speakers Dr. Cameron Clark, Neuropsychologist and Founder of Sharp Thinking, and ...
(Date:3/27/2021)... ... ... The Xtalks editorial team is pleased to announce the launch of the ... joined by editorial team members Ayesha Rashid, Sydney Perelmutter and Mira Nabulsi to discuss ... including insights from industry experts. , The Xtalks Life Science podcast will feature ...
(Date:3/23/2021)... Conn. (PRWEB) , ... March 23, 2021 , ... ... develops solutions for characterizing microbiome populations down to the strain level, recently unveiled ... applications. , Not all microbes are created equal: some are easy to ...
Breaking Biology Technology:
Cached News: