HOME >> BIOLOGY >> NEWS
Complex gene-swapping spawns a form of lymphoma

nockout mice failed to develop lymphomas. In addition, the researchers learned that the chromosome breaks involved in the lymphomas occurred where the RAG enzyme would normally snip apart DNA.

In a key set of experiments, the researchers used a technique called spectral karyotyping to analyze in detail the breakage and rearrangements of the chromosomes in the lymphomas. "Basically, this technique traces chromosome rearrangements by 'painting' individual chromosomes with fluorescent probes targeted to specific DNA sequences," said Alt. "If you paint different chromosomes different colors, you can figure out where the rearrangements came from."

Their analyses revealed that all the tumors showed a complex translocation -- a complicon -- that involved specific regions of chromosomes 12 and 15 that became embedded in a third chromosome. "We found that the amplified c-myc genes, as well as parts of the IgH locus, were somewhere else," said Alt. "They were on another chromosome that often had just pieces of chromosomes 12 and 15 attached. That was a surprise, because when we originally detected such a translocation between the two chromosomes, it resembled those of Burkitt's lymphoma which just involve human equivalents of 12 and 15, so we thought that was where the action would be in this case; but it wasn't."

Furthermore, said Alt, the chromosome breakages that caused amplification of the genes were not found in a location where one might expect them to trigger c-myc activation. "It was striking that every c-myc gene that we found to be amplified was located far away from the breakpoint, instead of adjacent to it, as is found in Burkitt's lymphoma." The adjacent breakage classically damages the regulatory region upstream of the gene, switching it on, explained Alt. "The mechanism that we have uncovered activates c-myc by leading to the amplification of the region of the chromosome in which it resides," he said.

In fac
'"/>

Contact: Jim Keeley
keeleyj@hhmi.org
301-215-8858
Howard Hughes Medical Institute
27-Jun-2002


Page: 1 2 3 4

Related biology news :

1. Complex cells likely arose from combination of bacterial and extreme-microbe genomes
2. UC Riverside to play active role in Institute for Complex Adaptive Matter
3. Complex carbohydrate found to regulate plant growth
4. The Geometry of Ecological Interactions: Simplifying Spatial Complexity
5. Complex mystery of amphibian decline calls for a new kind of science
6. Magnetic Fluids More Complex Than Previously Thought
7. Environmental Testimony: Taiwan Mega-Complex Threatens Endangered Spoonbills And Efforts To Cut Greenhouse Gases, UD Prof Says
8. Researcher Gains 30,000% Saving Using "Bargain Basement" Chemistry To Create Complex Industrially Useful Molecules
9. Second Gene Responsible For Tuberous Sclerosis Complex Identified
10. UCF brain cell research spawns hope for longer life
11. Sturgeon researchers vision spawns thriving industry

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/31/2016)... R.I. , March 31, 2016  Genomics firm ... of founding CEO, Barrett Bready , M.D., who ... members of the original technical leadership team, including Chief ... President of Product Development, Steve Nurnberg and Vice President ... returned to the company. Dr. Bready served ...
(Date:3/23/2016)... March 23, 2016 ... Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender Anbieter ... Unternehmen mit SpeechPro zusammenarbeitet, um erstmals dessen ... wird die Möglichkeit angeboten, im Rahmen mobiler ...
(Date:3/22/2016)... Ontario , PROVO and ... Newborn Screening Ontario (NSO), which operates the ... for molecular testing, and Tute Genomics and UNIConnect, ... management technology respectively, today announced the launch of a ... next-generation sequencing (NGS) testing panel. NSO ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... ... June 24, 2016 , ... While the majority of commercial spectrophotometers and fluorometers ... the 6000i models are higher end machines that use the more unconventional z-dimension of ... beam from the bottom of the cuvette holder. , FireflySci has developed several ...
(Date:6/23/2016)... 2016 /PRNewswire/ - FACIT has announced the creation ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or "the ... a portfolio of first-in-class WDR5 inhibitors for the ... WDR5 represent an exciting class of therapies, possessing ... for cancer patients. Substantial advances have been achieved ...
(Date:6/23/2016)... 2016  The Biodesign Challenge (BDC), a university competition ... harness living systems and biotechnology, announced its winning teams ... New York City . The ... projects at MoMA,s Celeste Bartos Theater during the daylong ... senior curator of architecture and design, and Suzanne ...
(Date:6/23/2016)... Apellis Pharmaceuticals, Inc. today announced positive ... its complement C3 inhibitor, APL-2. The trials were ... studies designed to assess the safety, tolerability, pharmacokinetics ... healthy adult volunteers. Forty subjects were ... dose (ranging from 45 to 1,440mg) or repeated ...
Breaking Biology Technology:
Cached News: