HOME >> BIOLOGY >> NEWS
Complex gene-swapping spawns a form of lymphoma

nockout mice failed to develop lymphomas. In addition, the researchers learned that the chromosome breaks involved in the lymphomas occurred where the RAG enzyme would normally snip apart DNA.

In a key set of experiments, the researchers used a technique called spectral karyotyping to analyze in detail the breakage and rearrangements of the chromosomes in the lymphomas. "Basically, this technique traces chromosome rearrangements by 'painting' individual chromosomes with fluorescent probes targeted to specific DNA sequences," said Alt. "If you paint different chromosomes different colors, you can figure out where the rearrangements came from."

Their analyses revealed that all the tumors showed a complex translocation -- a complicon -- that involved specific regions of chromosomes 12 and 15 that became embedded in a third chromosome. "We found that the amplified c-myc genes, as well as parts of the IgH locus, were somewhere else," said Alt. "They were on another chromosome that often had just pieces of chromosomes 12 and 15 attached. That was a surprise, because when we originally detected such a translocation between the two chromosomes, it resembled those of Burkitt's lymphoma which just involve human equivalents of 12 and 15, so we thought that was where the action would be in this case; but it wasn't."

Furthermore, said Alt, the chromosome breakages that caused amplification of the genes were not found in a location where one might expect them to trigger c-myc activation. "It was striking that every c-myc gene that we found to be amplified was located far away from the breakpoint, instead of adjacent to it, as is found in Burkitt's lymphoma." The adjacent breakage classically damages the regulatory region upstream of the gene, switching it on, explained Alt. "The mechanism that we have uncovered activates c-myc by leading to the amplification of the region of the chromosome in which it resides," he said.

In fac
'"/>

Contact: Jim Keeley
keeleyj@hhmi.org
301-215-8858
Howard Hughes Medical Institute
27-Jun-2002


Page: 1 2 3 4

Related biology news :

1. Complex cells likely arose from combination of bacterial and extreme-microbe genomes
2. UC Riverside to play active role in Institute for Complex Adaptive Matter
3. Complex carbohydrate found to regulate plant growth
4. The Geometry of Ecological Interactions: Simplifying Spatial Complexity
5. Complex mystery of amphibian decline calls for a new kind of science
6. Magnetic Fluids More Complex Than Previously Thought
7. Environmental Testimony: Taiwan Mega-Complex Threatens Endangered Spoonbills And Efforts To Cut Greenhouse Gases, UD Prof Says
8. Researcher Gains 30,000% Saving Using "Bargain Basement" Chemistry To Create Complex Industrially Useful Molecules
9. Second Gene Responsible For Tuberous Sclerosis Complex Identified
10. UCF brain cell research spawns hope for longer life
11. Sturgeon researchers vision spawns thriving industry

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/19/2016)... , UAE, April 20, 2016 ... implemented as a compact web-based "all-in-one" system solution for ... biometric fingerprint reader or the door interface with integration ... modern access control systems. The minimal dimensions of the ... readers into the building installations offer considerable freedom of ...
(Date:4/13/2016)... 13, 2016  IMPOWER physicians supporting Medicaid patients in ... new clinical standard in telehealth thanks to a new ... higi platform, IMPOWER patients can routinely track key health ... mass index, and, when they opt in, share them ... to a local retail location at no cost. By ...
(Date:3/23/2016)... , March 23, 2016 ... Interesse erhöhter Sicherheit Gesichts- und Stimmerkennung mit ... Inc. (NASDAQ: MESG ), ein ... dass das Unternehmen mit SpeechPro zusammenarbeitet, um ... der Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im ...
Breaking Biology News(10 mins):
(Date:5/3/2016)... ... 03, 2016 , ... Flagship Biosciences, the leader in ... Board of Directors. Dr. Gillett recently retired from Charles River Laboratories (CRL), where, ... Scientific Officer. A board-certified veterinary pathologist, Dr. Gillett joined Charles River in 1999 ...
(Date:5/3/2016)... 2016  Dr. Thomas P. McHugh , an ... The Woodlands, Texas , now offers SculpSure, the ... fat cells in just 25-minutes, leaving a slimmer figure ... of Americans report feeling bothered by excess weight and ... are a growing industry. This innovative new approach to ...
(Date:5/2/2016)... ... May 02, 2016 , ... Meister Media Worldwide launches ... look and added functionality give the agricultural world a taste of Meister Media ... in agriculture – from precision farming via satellites and Unmanned Aerial Vehicles (UAVs) ...
(Date:4/29/2016)... ... 29, 2016 , ... Proove Biosciences, Inc ., the ... launch of the Proove Health Foundation . The Foundation is a non-profit ... use of personalized medicine for tackling the nation’s most-pressing healthcare epidemics. As part ...
Breaking Biology Technology:
Cached News: