HOME >> BIOLOGY >> NEWS
Complex gene-swapping spawns a form of lymphoma

nockout mice failed to develop lymphomas. In addition, the researchers learned that the chromosome breaks involved in the lymphomas occurred where the RAG enzyme would normally snip apart DNA.

In a key set of experiments, the researchers used a technique called spectral karyotyping to analyze in detail the breakage and rearrangements of the chromosomes in the lymphomas. "Basically, this technique traces chromosome rearrangements by 'painting' individual chromosomes with fluorescent probes targeted to specific DNA sequences," said Alt. "If you paint different chromosomes different colors, you can figure out where the rearrangements came from."

Their analyses revealed that all the tumors showed a complex translocation -- a complicon -- that involved specific regions of chromosomes 12 and 15 that became embedded in a third chromosome. "We found that the amplified c-myc genes, as well as parts of the IgH locus, were somewhere else," said Alt. "They were on another chromosome that often had just pieces of chromosomes 12 and 15 attached. That was a surprise, because when we originally detected such a translocation between the two chromosomes, it resembled those of Burkitt's lymphoma which just involve human equivalents of 12 and 15, so we thought that was where the action would be in this case; but it wasn't."

Furthermore, said Alt, the chromosome breakages that caused amplification of the genes were not found in a location where one might expect them to trigger c-myc activation. "It was striking that every c-myc gene that we found to be amplified was located far away from the breakpoint, instead of adjacent to it, as is found in Burkitt's lymphoma." The adjacent breakage classically damages the regulatory region upstream of the gene, switching it on, explained Alt. "The mechanism that we have uncovered activates c-myc by leading to the amplification of the region of the chromosome in which it resides," he said.

In fac
'"/>

Contact: Jim Keeley
keeleyj@hhmi.org
301-215-8858
Howard Hughes Medical Institute
27-Jun-2002


Page: 1 2 3 4

Related biology news :

1. Complex cells likely arose from combination of bacterial and extreme-microbe genomes
2. UC Riverside to play active role in Institute for Complex Adaptive Matter
3. Complex carbohydrate found to regulate plant growth
4. The Geometry of Ecological Interactions: Simplifying Spatial Complexity
5. Complex mystery of amphibian decline calls for a new kind of science
6. Magnetic Fluids More Complex Than Previously Thought
7. Environmental Testimony: Taiwan Mega-Complex Threatens Endangered Spoonbills And Efforts To Cut Greenhouse Gases, UD Prof Says
8. Researcher Gains 30,000% Saving Using "Bargain Basement" Chemistry To Create Complex Industrially Useful Molecules
9. Second Gene Responsible For Tuberous Sclerosis Complex Identified
10. UCF brain cell research spawns hope for longer life
11. Sturgeon researchers vision spawns thriving industry

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/19/2016)... DUBAI , UAE, April 20, 2016 ... can be implemented as a compact web-based "all-in-one" system ... in the biometric fingerprint reader or the door interface ... requirements of modern access control systems. The minimal dimensions ... the ID readers into the building installations offer considerable ...
(Date:4/15/2016)... -- Research and Markets has announced the ...  report to their offering.  ,      ... gait biometrics market is expected to grow at ... Gait analysis generates multiple variables such ... compute factors that are not or cannot be ...
(Date:4/13/2016)...  IMPOWER physicians supporting Medicaid patients in ... standard in telehealth thanks to a new partnership with ... IMPOWER patients can routinely track key health measurements, such ... and, when they opt in, share them with IMPOWER ... local retail location at no cost. By leveraging this ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... , June 27, 2016  Global demand for ... percent through 2020 to $7.2 billion.  This market ... beverages, cleaning products, biofuel production, animal feed, and ... diagnostics, and biocatalysts). Food and beverages will remain ... by increasing consumption of products containing enzymes in ...
(Date:6/27/2016)... ... 2016 , ... Cancer experts from Austria, Hungary, Switzerland, and ... new and helpful biomarker for malignant pleural mesothelioma. Surviving Mesothelioma has just published ... , Biomarkers are components in the blood, tissue or body fluids that ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, ... faculty of the University of North Carolina Kenan-Flagler Business School effective ... at UNC Kenan-Flagler, with a focus on the school’s international efforts, leading classes ...
(Date:6/24/2016)... on a range of subjects including policies, debt and investment ... Speaking at a lecture to the Canadian Economics ... the country,s inflation target, which is set by both the ... "In certain areas there needs to be frequent ... not sit down and address strategy together?" He ...
Breaking Biology Technology:
Cached News: