HOME >> BIOLOGY >> NEWS
Computer model predicts outcome of DNA shuffling

University Park, PA --- Industries using DNA shuffling to improve enzymes, therapeutic proteins, vaccines and viral vectors may soon have a computational method for predicting the number and likely locations of crossovers, according to a Penn State research team.

"To date, the application of these methods has been based on experience and empirical methods and there was no model to understand the process which can be time consuming, expensive and of uncertain outcome," says Costas D. Maranas, assistant professor of chemical engineering. "We used thermodynamics and reaction engineering to evaluate and model this complex reaction network so we can now predict where the DNA from different parent genes will recombine."

DNA shuffling uses related genes from different species or genes with related function, fragments them and reassembles then through recombination. Researchers then place recombined genes into Escherichia coli to identify which new genes produce usable or potentially interesting products. Those genes that express a potentially interesting protein or enzyme are again fragmented and reassembled to form new recombinant genes. The process continues until a protein with the desired qualities is found.

"Beginning with genes that produce enzymes that are moderately good detergents, for example, the process iteratively searches for enzymes that are better detergents," says Maranas. The important factors in creating recombined genes with DNA shuffling include the temperature at which annealing joining of single-stranded DNA induced by cooling occurs, the similarity of the genes and the size of the DNA fragments. The computer program developed by Maranas; Gregory L. Moore, graduate student in chemical engineering; Stefan Lutz, postdoctoral fellow in chemistry; and Stephen J. Benkovic, the University professor, the Evan Pugh Professor of Chemistry and holder of the Eberly Chair in Chemistry, was described in the March 13
'"/>

Contact: A'ndrea Elyse Messer
aem1@psu.edu
814-865-9481
Penn State
12-Mar-2001


Page: 1 2 3

Related biology news :

1. Computer scientists at UH developing nurturing computers
2. Computer models expose humans as main cause of caribou decline
3. Computer database being developed at Temple will allow for better inventory of chemicals
4. Computer imaging of Archeopteryx skull suggests this dinosaur-bird link could fly
5. Computer model shows increased US threat from soybean rust
6. 20 May AAAS lecture: Computers that Respond to Human Emotion
7. Computer modeling targets epidemics, bioterror
8. Computer method identifies potentially active enzymes
9. Computer scientists develop tool for mining genomic data
10. Computer model offers new tool to probe Woburn toxic waste site
11. Computer models forecast sharp increase in temperature if heat-trapping emissions continue to rise

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/27/2021)... ... March 25, 2021 , ... Phlexglobal announced ... Planet Group, has selected Phlexglobal and its innovative regulatory SaaS software, PhlexSubmission, as ... a comprehensive review of five regulatory software companies, with the review team including ...
(Date:3/27/2021)... ... 25, 2021 , ... The 2021 Virtual Conference on Clinical Trial Supply-Europe ... More and more, clinical trial supply conferences are featuring speakers and forums that ... Asymmetrex’s founder and CEO, James L. Sherley, M.D., Ph.D., presented a talk ...
(Date:3/23/2021)... ... March 23, 2021 , ... G-CON Manufacturing (G-CON), the ... by Matica Biotechnology (Matica Bio), a contract development and manufacturing organization (CDMO) specializing ... the cleanroom build out for its new GMP production facility in College Station, ...
Breaking Biology News(10 mins):
(Date:3/27/2021)... ... March 24, 2021 , ... ... Life Sciences and Healthcare firms of all sizes, adds depth to its team ... specialist. Pardillo, who earned his doctorate in computational chemistry from Florida International University ...
(Date:3/27/2021)... ... March 24, 2021 , ... ABI Wellness, ... and reporting approach designed under CEO Mark Watson, today announced a webinar dedicated ... featuring guest speakers Dr. Cameron Clark, Neuropsychologist and Founder of Sharp Thinking, and ...
(Date:3/27/2021)... ... ... The Xtalks editorial team is pleased to announce the launch of the ... joined by editorial team members Ayesha Rashid, Sydney Perelmutter and Mira Nabulsi to discuss ... including insights from industry experts. , The Xtalks Life Science podcast will feature ...
(Date:3/23/2021)... Conn. (PRWEB) , ... March 23, 2021 , ... ... develops solutions for characterizing microbiome populations down to the strain level, recently unveiled ... applications. , Not all microbes are created equal: some are easy to ...
Breaking Biology Technology:
Cached News: