Crime scene or nature reserve?

A method used by forensic experts to collect evidence from crime scenes could soon be taken up by biologists studying animals in the wild. An article in BMC Ecology this week describes how DNA from animal blood and tissue samples can be stored on record cards made from specialist filter paper and used in experiments at a later date.

"Techniques involving the analysis of DNA have become ubiquitous in many areas of wildlife research," write Lee Smith and Leigh Burgoyne, who carried out the study. "Unfortunately the transport of fresh samples from the point of collection involves leakage risks and the possibility of sample degradation due to temperature variation."

To avoid these problems the Australian researchers suggest collecting DNA samples on squares of specialist filter paper, called FTA databasing paper, produced by Whatman. This paper is impregnated with a mix of chemicals that lyse cells, prevent bacterial growth and protect DNA from degradation.

The scientists outline methods that can be used to collect DNA from a variety of samples, including blood, blood clots, tissue extracts and cheek swabs. They go on to detail how these samples can be processed, taking account of several problems associated with samples of wildlife origin.

The basic premise is simple: Biological samples are applied to FTA paper and air-dried. A small disc is then removed from the card, and washed to remove everything except the DNA, which remains entangled with the fibres of the paper. Analyses of the genetic material can be performed while it is still attached to the disc. Alternatively, the DNA can be washed off the disc with solvents before it is used in experiments.

Smith explains one of the many benefits of using this technique: "You can extract usable DNA from very small quantities of blood. Five micro-litres are more than enough for between five and ten analyses which makes live sampling much less invasive."

To test the stability o

Contact: Gemma Bradley
BioMed Central

Page: 1 2

Related biology news :

1. Crime scene to court
2. Loyola decides to test new blood substitute in trauma patients at the scene of injury
3. Getting adults to exercise may hinge on providing paths and pretty scenery
4. Global change conference sets the scene for next round of Kyoto Protocol talks
5. When is a pollinator not a pollinator? Tales from an insect crime scene...
6. Researchers identify distinctive signature for metastatic prostate cancer
7. Entrepreneurs to be told how natures secrets can improve business
8. Forest fire sensor inspired by nature
9. Survival strategies in nature
10. Jefferson researchers develop microchip to track genetic signature of cancer and normal tissue
11. ESAs minature Earth observer put to many uses

Post Your Comments:

(Date:5/6/2020)... ... May 06, 2020 , ... The LaunchPort™ ... the latest out-of-state company to partner with the device accelerator located in ... a point-of-care analyzer capable of accurately performing enzyme-linked immunosorbent assays (ELISAs), the ...
(Date:5/1/2020)... ... 2020 , ... InBios International Inc., a leading developer of ... a contract from the Biomedical Advanced Research and Development Authority (BARDA), part of ... Department of Health and Human Services, to develop the SCoV-2 Detect IgM/IgG Rapid ...
(Date:4/30/2020)... , ... April 28, 2020 , ... ... are Lachman Consultants latest effort in providing timely and critical information to life ... by social distancing and health safety concerns. , “Providing the most up-to-date actionable ...
Breaking Biology News(10 mins):
(Date:5/14/2020)... (PRWEB) , ... May 13, 2020 , ... ... hub called Future Lab: Optimizing Workflows in the Life Science Lab . ... workflows in their lab, from water purification and liquid handling to contamination control. ...
(Date:5/9/2020)... LOS ANGELES (PRWEB) , ... May 08, 2020 , ... ... week’s episode of their successful business program on Sunday, May 10, 2020 and Wednesday, ... each U.S. coast! This episode’s exclusive interviews will spotlight key executives from Techolution ...
(Date:5/5/2020)... ... May 05, 2020 , ... ProductLife Group has marked ... Duburcq joins the group after 13 years at Altran, where he was Group VP ... in Immunology and being a Pharmacist, Xavier was involved in his early career in ...
(Date:5/1/2020)... ... April 30, 2020 , ... The winners of ... policies, projects, and concepts that are actively engaged and deeply committed to changing ... has taken out the top prize in the Experimental category. , The RASTRUM™ ...
Breaking Biology Technology:
Cached News: