DNA Enzyme Works In Crystal Form; Yields New Insights Into Gene Copying

DURHAM, N.C. -- Duke University Medical Center biochemists have made the startling discovery that an enzyme that copies DNA in living cells can also be made to operate when held in place in crystal form.

Their achievement opens the way for understanding the finest details of how the intricate DNA-copying enzyme -- called DNA polymerase -- manages to reproduce DNA with the impeccable accuracy necessary for all living things to grow and reproduce nearly flawlessly.

By shining X-rays through the actively functioning crystals, the biochemists have taken snapshots that catch the enzyme in the biochemical act of copying DNA. Soon they will make movies. The scientists' images also are revealing details of how cancer-causing chemicals interfere with the copying process.

The biochemists, led by assistant professor of biochemistry Lorena Beese, published their discovery in the Jan. 15 issue of Nature, in an article titled "Visualizing DNA Replication in a Catalytically Active Polymerase at 1.8 Angstrom Resolution." Besides Beese, authors of the paper are graduate student James Kiefer and postdoctoral associate Chen Mao of Duke, and researcher Jeffrey Braman of Stratagene of La Jolla, Calif.

The scientists' work was supported by the American Cancer Society, the Searle Scholar Foundation, and the North Carolina Biotechnology Center.

Enzymes are proteins that are the workhorses of the cell, catalyzing the multitude of chemical reactions that underlie all cell functions. The molecules that enzymes act upon are called substrates.

The DNA polymerase that the Duke researchers studied is part of a complex molecular assembly line that is central to all cell division. A cell preparing to divide first unzips its double-stranded DNA into a single strand, to prepare for DNA copying. DNA polymerase then attaches to one strand, using it as a template. The polymerase works its way along the strand, chemical

Contact: Dennis Meredith
Duke University

Page: 1 2 3 4 5

Related biology news :

1. Enzyme maintaining chromosome ends is linked to bone cancer recurrence, decreased survival
2. Enzyme activation appears key in helping internal clock tell night from day
3. Enzyme fully degrades mad cow disease prion
4. Enzyme revealed that is key to funguss ability to breach immune system
5. Enzyme discovery sheds light on causes of rare disease, cancer
6. Enzyme could overcome industrial bleaching waste problems
7. Enzyme controls good cholesterol
8. Enzyme discovery to benefit homeland security, industry
9. Enzyme could aid cancer fight
10. Enzyme once thought harmful to Alzheimers patients now appears key to future treatment
11. Enzyme mimetic reduces tissue damage in colitis animal study

Post Your Comments:

(Date:8/3/2020)... ... 2020 , ... Accumen Inc. is helping healthcare get better, ... Solutions, to provide COVID-19 saliva testing kits nationally. , “Accumen has been ... 10 years. Early in the COVID-19 crisis, we identified supply chain gaps and ...
(Date:7/18/2020)... ... ... Commercial launch readiness is a critical stage in a product life cycle. While ... global economic downturn will only increase price pressures overall for the industry. The era ... from every product launch is critical. However, history shows that only a third of ...
(Date:7/2/2020)... ... July 02, 2020 , ... ... announced a publication detailing the use of its revolutionary NEXTGENPCR endpoint thermocycler ... (RT-PCR) in 16 minutes. The article, titled "Ultra-fast one-step RT-PCR protocol for ...
Breaking Biology News(10 mins):
(Date:7/18/2020)... ... July 16, 2020 , ... After research model surgery and catheter implantation, the ... is only the beginning of a successful study, while protecting and accessing the catheter ... Global Head of Surgery from Envigo in a live webinar ...
(Date:7/10/2020)... ... July 09, 2020 , ... PathSensors Inc., ... has expanded the company’s exclusive license to include clinical applications for CANARY™ ... market, focusing initially on the SARS-CoV-2 biosensor. CANARY’s™ fast and highly ...
(Date:7/1/2020)... ... 29, 2020 , ... MedShift , a leading medical ... many obstacles created as a result of COVID-19. As a provider at the ... practices and medical manufacturers by expanding access to device offerings, technology services, and ...
(Date:6/28/2020)... HILL, Conn. (PRWEB) , ... June 25, 2020 ... ... company formed to advance photodynamic therapy for treating cancer, today announced the company ... New York (“Roswell Park”). The agreement provides Lumeda globally exclusive rights to ...
Breaking Biology Technology:
Cached News: