Discovering what genes do the high-throughput way

by the screen to characterize the effects of the RNAi treatment on the cells. They created a formal set of criteria to judge the cells consisting of seven classes of change that could have been induced. These included changes to cell number, shape, size and viability. Any changes were only considered significant if both scientists recorded them in replica experiments.

The researchers were also keen to find out if their method could be used to screen for genes that worked in, or inhibited specific molecular pathways. Screens in whole flies for genes that modify the effect of a particular genetic mutation have proved powerful, though time consuming. By adding two sets of double stranded RNA to each well, one that targeted the tumor suppressor gene pten and the other the gene to be tested, the researchers found that they were able to identify genes that modified the effects of inhibiting pten in cells. "These results demonstrate that modifier screens, as previously done in vivo, can be extended to RNAi screening methodology in cell culture", write the researchers.

Drug companies are becoming interested in this technique, as the rate-limiting step in cell-based drug discovery is finding out which protein is inhibited by a particular drug. Using Perrimon's method they can screen to see which gene, when inhibited, changes the cell in the same way as adding the drug.

"RNAi screens can complement classical Drosophila genetics to assign functions to both known and novel genes," write the researchers. "The same technology can be easily adapted to a wide variety of cell-based studies and a greater genomic scale."

Baum says, "The major difference between this and whole fly screens is that here we can be systematic. We can choose to look at any cell biological process and systematically test the set of genes that could be involved. In the future we will be able to screen the full genome in a few weeks, and look at any cell biological phenomenon.

Contact: Gemma Bradley
BioMed Central

Page: 1 2 3

Related biology news :

1. Discovering new regulators of the immune system
2. Discovering the Tree of Life
3. Discovering Virology From Beijerinck To Hyperlinks
4. "Discovering Biological Diversity" Is AAAS Topic For USGS Manager
5. Belgian researchers explore revolutionary approach to angiogenesis
6. Tracing genes, biologists show lizard migration is traced to Florida
7. Certain genes boost fish oils protection against breast cancer
8. Fossil genes reveal how life sheds form and function
9. Missing genes may help explain why plague bacteria are so deadly
10. Do genes respond to global warming?
11. Genetic map of important tree genes outlined

Post Your Comments:
TAG: Discovering what genes the high throughput way

(Date:9/24/2015)... Publiceringsförbud fram till: ... Kerv ( http://kerv.com ), det Londonbaserade ... första kontaktlösa betalningsring på kickstarter.com i ett ... för massproduktion via crowdfunding.       ... Kerv-bärare kan göra direkta kontaktlösa betalningar ...
(Date:9/10/2015)... Sept. 10, 2015 Pursuant Health has ... to create an interactive, image-based health risk assessment ... wellness kiosk.  The unique assessment quantifies user results ... that suggests an individual,s biological age based on ... measured by the kiosk. Comprised of ...
(Date:9/8/2015)... , Sept. 8, 2015  Affectiva, global ... Emotion as a Service, a new data solution, ... Based on Affectiva,s highly accurate and patented ... analytics capabilities, slated to transform industries such as ... development, retail, customer experience, online education, HR, automotive, ...
Breaking Biology News(10 mins):
(Date:10/9/2015)... Governor Tom Wolf announced today that Adaptimmune ... T-cell receptor cancer immunotherapy treatments, will create at least ... Pennsylvania , and today,s announcement ... high-paying jobs will be created with this project," said ... sustained funding for the life science sector and increased ...
(Date:10/9/2015)... Oct. 9, 2015  DePuy Synthes Trauma* announced today ... Q-PACK ®  Technology**, the only pre-hydrated demineralized cancellous ... procedures (foot and ankle, hand and wrist), including fusion, ... here at the 2015 Orthopaedic Trauma Association (OTA) Annual ... scaffold for new bone growth) and osteoinductive 2,3 ...
(Date:10/9/2015)... ... October 09, 2015 , ... ... the current methods of separating those cells from their surroundings for research, diagnostics, ... the cells. , To address this, Ann Arbor-based startup Akadeum Life Sciences ...
(Date:10/8/2015)... ... October 08, 2015 , ... Researchers at the University of Washington (UW) ... providing a new tool to study how diseases like dementia, Alzheimer's, and brain tumors ... and Ruikang Wang of the UW Department of Bioengineering, today in the Journal ...
Breaking Biology Technology:
Cached News: