HOME >> BIOLOGY >> NEWS
Engineering New Enzymes

The architecture of proteins is intrinsically hierarchic. Secondary structural elements can form motifs or subdomains which assemble to domains. Domains are structurally and functionally independent, where as motifs or subdomains are stabilized by other parts of the protein and are not structurally and functionally independent.

The evolution of proteins has generated numerous functional domains where the active site resides at the boundary of two such subdomains. Consideration of these hierarchical features of the protein architecture by recombination of subdomains from different proteins would therefore open new approaches in the design of novel enzymatic functions and benefit many design problems.

A first attempt in this direction is the recombination of subdomains selected from homologous proteins which have diversified through evolution. In the August 18 issue of Proc. Natl. Acad. Sci. USA (vol. 95, Issue 17, 9813-9818) scientists from the Max Planck Institute of Biochemistry in cooperation with scientists from Boehringer Mannheim GmbH present a method to generate a hybrid gene by combining the gene segment for a subdomain from the digestive proteinase trypsin with a gene segment for a subdomain from the coagulation proteinase factor Xa. The hybrid protein was expressed in E. coli and folded into a functional serine proteinase as shown by X-ray crystallography and enzymatic assays. While its catalytic activity was comparable to that of trypsin and factor Xa, the hybrid protease displayed a much broader substrate specificity than trypsin and factor Xa, a property associated with "unevolved" or "ancient" enzymes.

This strategy allows one now to swap specific subdomain linked functions, to preserve conserved functions at the interface, and to generate new properties at non-conserved or hypervariable surfaces near
'"/>

Contact: Richard A. Engh
engh@biochem.mpg.de
49-89-8578-2630
Max-Planck-Gesellschaft
2-Sep-1998


Page: 1 2

Related biology news :

1. Engineering endurance: The future of the Olympics?
2. The amendment to the law on green Genetic Engineering inhibits innovation and research in Germany
3. The stinky smell of success of Ted the Titan: a tale from Chemical & Engineering News
4. Engineering a solution to waste crisis
5. Dana-Farber launches NFCR Center for Therapeutic Antibody Research and Engineering
6. Symposium by ISPRS and the U-M College of Engineering
7. Story Tips: Engineering Students Pursue Cutting-Edge Research
8. Duke Geologist Decries Using Engineering Models To Predict Natural Phenomena
9. Engineering Animation, Inc. Demonstrates Internet Anatomy At Visible Human Project Conference
10. Engineering, Math Shows Short-Legged Human Ancestors Were Very Efficient At Strolling Through Their World
11. Conservation Biology Clearinghouse, Engineering Design Tutor

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/26/2020)... ... August 24, 2020 , ... ... of chronic inflammatory and fibrotic diseases, today announced that Dr. Stacie Canan has ... Discovery. , Dr. Canan previously served as Executive Director of Global Health at ...
(Date:8/26/2020)... HOUSTON (PRWEB) , ... August 25, 2020 , ... ... it made Inc. magazine’s 2020 annual Inc. 5000 list. The list is the most ... list in 2019 as well, and for this year’s list, reported three-year revenue growth ...
(Date:8/21/2020)... ... August 19, 2020 , ... “How can we ... dedicated to Salivary Bioscience for more than twenty years. Together with Douglas Granger, ... " Salivary Bioscience: Foundations of Interdisciplinary Saliva Research and Applications ," and Steven ...
Breaking Biology News(10 mins):
(Date:8/12/2020)... (PRWEB) , ... August 12, 2020 , ... ... Inc. (EMMA International), a global leader in FDA compliance consulting has been ... nation’s fastest-growing private companies. The list represents a unique look at the most ...
(Date:7/31/2020)... ... ... R3 Stem Cell International is now offering patients the opportunity to receive ... patients may choose which extremities they would like treated. , Several studies have shown ... At R3 International, umbilical cord tissue is obtained from a lab with a long ...
(Date:7/31/2020)... (PRWEB) , ... July 29, 2020 , ... ... and Catalent, the leading global provider of advanced delivery technologies, development, and manufacturing ... announced that they have entered into a strategic partnership whereby Catalent will provide ...
(Date:7/22/2020)... ... July 22, 2020 , ... Join experts from Reed Tech ... Regulatory Solutions, in a one hour live webinar on Thursday, August 13, ... China for drugs and medical devices. Specifically, for medical devices, the NMPA has departments ...
Breaking Biology Technology:
Cached News: