HOME >> BIOLOGY >> NEWS
Enzyme once thought harmful to Alzheimers patients now appears key to future treatment

peptide was undertaken in the cholinergic basal forebrain. A team of researchers investigated the actions of galanin on acutely dissociated rat cholinergic basal forebrain neurons from the nucleus of the diagonal band of Broca (DBB) using a combination of whole cell patch-clamp and single-cell reverse transcription-polymerase chain reaction (RT-PCR) analysis.

The authors of "Novel Excitatory Actions of Galanin on Rat Cholinergic Basal Forebrain Neurons: Implications for Its Role in Alzheimer's Disease," are Jack H. Jhamandas, Kim H. Harris, David MacTavish, and Balvinder S. Jassar, all from the Division of Neurology, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada. Their findings appear in the current edition of The American Physiological Societys Journal of Neurophysiology.

Methodology
Brains were quickly removed from decapitated male rats and placed in cold artificial cerebrospinal fluid that contained a mixed solution. Brain slices were cut and the area containing the DBB was dissected out. Although most of the tissue contained the horizontal limb of the DBB, some slices may have included a component of the vertical limb of the DBB.

Acutely dissociated neurons were prepared by enzymatic treatment of slice with trypsin, followed by mechanical conversion to a fine powder for dispersion of individual cells. Cells were then plated on poly-L-lysine-coated cover slips and viewed under an inverted microscope. All solutions were kept oxygenated by continuous bubbling with pure oxygen.

Results
The findings provide the first electrophysiological evidence that the neuropeptide galanin causes a response from central neurons found in mammals. Activation of galanin receptors results in membrane depolarization and an increase in excitability of basal forebrain neurons, which is related in part, through its effects in reducing specific voltage- and calcium-dependent potassium conductances. Furthermore,
'"/>

Contact: Donna Krupa
djkrupa1@aol.com
703-527-7357
American Physiological Society
14-Mar-2002


Page: 1 2 3

Related biology news :

1. Enzyme maintaining chromosome ends is linked to bone cancer recurrence, decreased survival
2. Enzyme activation appears key in helping internal clock tell night from day
3. Enzyme fully degrades mad cow disease prion
4. Enzyme revealed that is key to funguss ability to breach immune system
5. Enzyme discovery sheds light on causes of rare disease, cancer
6. Enzyme could overcome industrial bleaching waste problems
7. Enzyme controls good cholesterol
8. Enzyme discovery to benefit homeland security, industry
9. Enzyme could aid cancer fight
10. Enzyme mimetic reduces tissue damage in colitis animal study
11. Enzyme studies at Brookhaven Lab may lead to new antiviral agents

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/27/2019)... , ... April 26, 2019 , ... ... Plan Competition on Thursday, April 25. More than 30 student venture teams, representing ... prizes totaling more than $75,000. , The top five teams in the ...
(Date:4/25/2019)... ... April 23, 2019 , ... In recent years the neurocognitive ... However, in many cases the magnitude of these deficits and the long-term impact ... given neurocognitive effects may impact developmental trajectories, and patients are often so unwell ...
(Date:4/25/2019)... ... April 24, 2019 , ... Noblis, Inc., a leading provider ... 10,191,929, “Systems and Methods for SNP Analysis and Genome Sequencing.” Noblis’ new system ... of advanced bioinformatic analyses, including SNP calling, metagenomic analysis, and conserved and signature ...
Breaking Biology News(10 mins):
(Date:4/16/2019)... ... 2019 , ... On April 24th, the Federal Laboratory Consortium ... for Environmental Health Research (USACEHR), with an award for their cooperative effort in ... company located at the Frederick Innovative Technology Center, Inc. (FITCI) on Metropolitan Court, ...
(Date:4/10/2019)... FREDERICK, Md. (PRWEB) , ... ... ... and GenCure, both regenerative medicine manufacturing technology leaders, have locked into multiple ... than ever before. Companies across the regenerative medicine industry, ranging from synthetic ...
(Date:4/9/2019)... ... 2019 , ... The American Society of Gene & Cell Therapy (ASGCT) ... a new addition to the presidential line of succession, at the 22nd Annual ... leadership team for a three-year term (2019-2021). , All five incoming members of the ...
(Date:4/4/2019)... ... April 02, 2019 , ... Biomeme, ... be releasing it’s latest field-ready solution for performing PCR, RT-PCR, qPCR, and Isothermal ... pays homage to Rosland Franklin, an English chemist and X-ray crystallographer who made ...
Breaking Biology Technology:
Cached News: