HOME >> BIOLOGY >> NEWS
Findings in frog oocytes may help study of chromosome physiology

CHAMPAIGN, Ill. -- Researchers studying the nuclei of frog oocytes in early stages of meiosis -- the cell division that gives rise to germ cells -- have found that two key proteins remain apart at a crucial time before condensation occurs. One of the proteins, they say, may be important in the early organization of chromosomes and later may recruit the other.

In the August issue of the journal Chromosome Research, scientists at the University of Illinois at Urbana-Champaign detail how they used antibodies to the proteins, XCAP-E and XCAP-D2, and confocal laser scanning microscopy to zero in on the proteins' precise locations inside the nuclei of gametes of the African frog Xenopus laevis.

(The research will be on display as a poster presentation Aug. 17-22 during the Gorden Research Conference on Plasmid & Chromosome Dynamics in Tilton, N.J.)

The proteins are from two subcomplexes of chromosome condensation proteins known as condensins. One group of the Xenopus Chromosome Associated Proteins (XCAP), including XCAP-D2, consists of regulatory proteins, while XCAP-E is among those directly involved in condensation.

"On the chromosomes, XCAP-D2 was only found associated with the chromomeres," said Michel Bellini, a professor of cell and structural biology at Illinois. That location features highly compacted chromatin, or fibers of DNA, suggesting XCAP-D2 is directly involved early in meiotic chromatin organization.

Immunofluorescent staining showed XCAP-D2 proteins on the 18 easily recognizable chromosomes of the frog oocyte. These extended chromosomes stretch out long loops of chromatin -- regions of extensive RNA synthesis -- causing them to resemble a lampbrush, and, more importantly, allowing for detailed cytological studies of the major components implicated in both RNA transcription and chromatin organization.

"When we looked for XCAP-E, we did not see it on the chromosomes," Bellini said. "Instead, it was accum
'"/>

Contact: Jim Barlow
jebarlow@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
14-Aug-2003


Page: 1 2 3

Related biology news :

1. Findings suggest need for new view of p53 cancer proteins interaction with DNA
2. Findings redefine mechanism of action of RNA helicase enzymes
3. Findings of novel nanoproperties in selenium produced by bacteria open new area of exploration
4. Findings could aid efforts to harness nature for making drugs
5. Findings offer clue to how molecule can both stimulate, suppress cell growth
6. Findings offer further understanding about growth and development in young male gymnasts
7. Findings aid understanding of neurodegenerative diseases
8. Findings show exceptional longevity runs in families
9. Findings give insight into how colon cells transform into precancerous polyps
10. Findings conclude sustained caffeine intake negates the benefits of creatine supplements
11. Findings link disease specific anitbodies to activation of T cells for the first time

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/23/2019)... (PRWEB) , ... March 21, 2019 , ... ... growth in recent years with a particular focus on machine learning. Machine learning ... data through a process called ‘learning’ or ‘training’. Through machine learning, scientists are ...
(Date:3/18/2019)... and BERKELEY, Calif. (PRWEB) , ... March 18, ... ... Pivot Bio, the leader in microbial solutions announce today they have ... Pivot Bio PROVENTM, Pivot Bio’s breakthrough product for biologic nitrogen fixation for corn. ...
(Date:3/13/2019)... VIEW, Calif. (PRWEB) , ... March 13, 2019 , ... ... up to 45 percent of the world’s population. One billion people ... breathing is interrupted during sleep - more than 80 percent of whom go undiagnosed. ...
Breaking Biology News(10 mins):
(Date:3/14/2019)... LAWRENCEVILLE, N.J. (PRWEB) , ... ... ... announced the launch of a preclinical gold standard solution for preclinical research ... Execution System (LES) with end-to-end workflows, connected systems and facilities, innovative technology-driven ...
(Date:3/12/2019)... ... March 12, 2019 , ... ... research, has launched its third generation long-fragment DNA shearing innovation, the Megaruptor 3. ... a user-friendly interface, automated processing, and the ability to process up to ...
(Date:3/11/2019)... ... March 11, 2019 , ... USDM Life Sciences and Generis ... with built in GxP compliance for the life sciences industry. USDM Cloud Assurance ... continuous compliance for Generis’ flagship product, CARA. , Presentation One:, ...
(Date:3/9/2019)... ... March 08, 2019 , ... ... and higher education planning, announced the release of their Best Online Master's ... the nation based on curriculum quality, program flexibility, affordability, and graduate outcomes. ...
Breaking Biology Technology:
Cached News: