HOME >> BIOLOGY >> NEWS
First production of a human protein with complex glycosylation in genetically modified yeast

ting safer, more effective therapeutics."

Humanizing the Yeast Glycosylation Pathway

The GlycoFi team began their work with the yeast P. pastoris, a robust organism commonly used in fermentation processes, which can be grown to high cell density in a chemically defined growth medium. This yeast normally produces non-human N-glycans of the high mannose type, which have no therapeutic value for humans. The scientists modified the yeast by first eliminating endogenous yeast glycosylation pathways, while sequentially engineering into the organism five active eukaryotic proteins, including mannosidases I & II, N-acetylglucosaminyl transferases I & II and UDP-N-acetylglucosamine transporter. The targeted localization of these enzymes enabled the generation of a synthetic in vivo glycosylation pathway that enabled the yeast to produce a complex human N-glycan, GlcNAc2Man3GlcNAc2, in vivo. However, unlike the glycosylation pathway in mammalian cell lines, which typically produces an array of glycoforms, the genetically modified yeast yielded essentially homogeneous glycoforms.

"We have essentially been able to humanize the yeast, where it is able to make a single glycoform of exceptional uniformity," Dr. Gerngross said. "Unfortunately, using mammalian cell culture, it is difficult to isolate individual protein glycoforms and even more difficult to produce specific structures at a commercial scale. The ability to express a single protein in a library of genetically engineered yeasts each producing a defined and uniform glycoform will enable the generation of glycoprotein libraries that can be used both to elucidate specific structure-function relationships and to identify the most efficacious molecule for a particular therapeutic use. Moreover, once identified, a particular protein glycoform can be readily produced at industrial scale using the relevant yeast, due to the well-established rapidity with which yeast fermentations can be scaled u
'"/>


28-Aug-2003


Page: 1 2 3

Related biology news :

1. First glimpse of DNA binding to viral enzyme
2. First clinical study of new pediatric croup vaccine shows safety, tolerability in adults
3. 2nd media alert First Scientific Conference on Childhood Leukaemia
4. First International Scientific Conference on Childhood Leukaemia
5. First ever standards linking climate change, biodiversity and poverty seek global peer review
6. First genetic comparison of purebred domestic dogs produces surprises
7. First time in the U.S.: Saint Louis University tests third-generation vaccine against smallpox
8. First target for childhood malaria vaccine
9. First study of resveratrol dietary supplement finds effect on breast and prostate cancers unlikely
10. First flavors form a lasting impression
11. STN International launches Derwent World Patents Index First View

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/26/2020)... , ... August 25, 2020 ... ... biotechnology company developing novel pharmaceuticals targeting mitochondria, in partnership with Essex Bio-Technology, ... in its pivotal U.S. Phase 3 VISTA-2 study of SkQ1 ophthalmic solution ...
(Date:8/26/2020)... HOUSTON (PRWEB) , ... August 25, 2020 , ... ... it made Inc. magazine’s 2020 annual Inc. 5000 list. The list is the most ... list in 2019 as well, and for this year’s list, reported three-year revenue growth ...
(Date:8/21/2020)... Mass. (PRWEB) , ... August 18, 2020 , ... ... approaches to cell therapy, today announced that the U.S. Food and Drug Administration ... of Sentien’s lead asset, SBI-101, for the treatment of severe COVID-19. Approval of ...
Breaking Biology News(10 mins):
(Date:7/22/2020)... ... 2020 , ... Join experts from Reed Tech , Gary Saner, Sr. ... one hour live webinar on Thursday, August 13, 2020 at 11am EDT ... medical devices. Specifically, for medical devices, the NMPA has departments dealing with medical device ...
(Date:7/10/2020)... LORTON, Va. (PRWEB) , ... July 08, 2020 ... ... laboratory testing services and products, announces a significant expansion of laboratory operations through ... robust collection for agencies implementing testing programs. , Bode-CARES provides ...
(Date:7/7/2020)... ... July 06, 2020 , ... Bio-IT ... Roche, Eli Lilly, Bristol-Myers Squibb, the University of Chicago, Massachusetts General Hospital, Mission: ... hosted an elite awards program, highlighting outstanding examples of how technology innovations and ...
(Date:6/28/2020)... , ... June 25, 2020 , ... ... racing to develop a vaccine or drug treatment. In an effort to better ... has publicly released the world’s largest imaging dataset portraying therapeutic compound effects from ...
Breaking Biology Technology:
Cached News: