HOME >> BIOLOGY >> NEWS
Freezing human eggs - new findings point way to greater success

Freezing human eggs has proved one of the most difficult techniques to master in reproductive medicine and only about thirty babies have been born worldwide since the first attempts in 1986. But research published today*(Tuesday 27 February) in Europe's leading fertility journal, Human Reproduction, could lead to a significant improvement in success rates.

Italian fertility experts lead by Dr Rafaella Fabbri from the IVF Centre, Human Reproductive Medicine Unit at the University of Bologna, have identified crucial factors in the freeze-thaw process which contribute to the difference between success and failure. Putting their findings into practice in a laboratory study, they achieved a much higher than normal rate of egg survival and fertilised 57% by using ICSI (the injection of a single sperm into the egg). This is a figure comparable to fertilisation of fresh oocytes.

The key to their success was to increase the time the oocytes were exposed to the chemical protectants, propanediol plus sucrose, from around 10 minutes to 15 minutes and to double or even triple the normal amount of sugar (sucrose) in the freezing solution.

The main reason that human eggs disintegrate in the freezing process is inadequate dehydration. Longer exposure to the protectant and more help from the extra sucrose meant that the eggs were dehydrated gradually and more completely. This gentler freezing process minimised the formation of ice crystals, which can pierce the egg membrane and kill the cell.

The researchers further established that one factor thought to be important in successful cryopreservation - the presence of the protective nourishing cells surrounding the oocyte (cumulus oophorus) - did not actually have a bearing on success. There was no real difference in survival between eggs frozen with their cumulus oophorus partially removed or totally removed.

But, increasing the exposure time to the protectant and doubling the sucrose solution in the freezin
'"/>

Contact: Margaret Willson
m.willson@mwcommunications.org.uk
44-0-1536-772181
European Society for Human Reproduction and Embryology
26-Feb-2001


Page: 1 2 3 4

Related biology news :

1. Freezing cancer cells makes them prime targets for anti-cancer drug, new study finds
2. Scientists Trace Roots Of Protein That Keeps Antarctic Fish From Freezing
3. Climate change plus human pressure caused large mammal extinctions in late Pleistocene
4. Lycopene slows human prostate tumour growth in mice and combined with vitamin E is even better
5. Most recent common ancestor of all living humans surprisingly recent
6. Wildlife Conservation Society hosts public symposium on human-wildlife diseases
7. Bronfenbrenner book sums up human development
8. No role for simian virus 40 in human pleural mesotheliomas
9. Sugar-coated sea urchin eggs could have sweet implications for human fertility
10. Serotonin metabolites in mollusks suggest pathways for human therapies
11. Wrapping a memory with an experience, capacity for recollection detected in non-human species

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/7/2016)... --  Veridium , a leader in biometrics-based authentication ... James Stickland . Stickland, a seasoned financial ... served in senior executive roles for HSBC, JPMorgan ... a pipeline of venture capital and accelerating innovation ... as managing director of U.K.-based fintech firm Red ...
(Date:12/6/2016)... Valencell , the leading innovator in ... a third consecutive year of triple digit growth for ... with a 360 percent increase in companies who have ... driven by sales of its wrist and ear Benchmark™ ... technology for hearables for fitness and healthcare applications. ...
(Date:12/5/2016)... , Dec. 5, 2016  The Office ... today published "Can CT Scans Enhance or Replace ... the potential of supporting or replacing forensic autopsies ... CT scan. In response to recommendations ... is exploring using CT scans as a potential ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... DES MOINES, Iowa , Dec. 8, 2016 Eurofins ... for US Food and President of Eurofins Scientific Inc. (ESI). ... Food Division with his proven professional and entrepreneurial experience in leading ... growth in the US food testing market to uphold Eurofins, status ... ...
(Date:12/8/2016)... , December 8, 2016 ... expanded its customisable SureSeq™ NGS panel range with the launch ... fast and cost-effective study of variants in familial hypercholesterolemia (FH). ... variation (CNV) detection on a single small panel and allows ... content. This includes all exons for LDLR , ...
(Date:12/8/2016)... ... December 08, 2016 , ... ... World Technology Awards. uBiome is one of just six company finalists in the ... In addition to uBiome, companies nominated as finalists in this year’s awards include ...
(Date:12/8/2016)... Dec. 8, 2016  HedgePath Pharmaceuticals, Inc. (OTCQX: ... develops and plans to commercialize innovative therapeutics for ... common stock were approved for trading on the ... on the OTCQX, effective today, under the ticker ... OTCQX market, companies must meet high financial standards, ...
Breaking Biology Technology:
Cached News: