HOME >> BIOLOGY >> NEWS
Freezing human eggs - new findings point way to greater success

Freezing human eggs has proved one of the most difficult techniques to master in reproductive medicine and only about thirty babies have been born worldwide since the first attempts in 1986. But research published today*(Tuesday 27 February) in Europe's leading fertility journal, Human Reproduction, could lead to a significant improvement in success rates.

Italian fertility experts lead by Dr Rafaella Fabbri from the IVF Centre, Human Reproductive Medicine Unit at the University of Bologna, have identified crucial factors in the freeze-thaw process which contribute to the difference between success and failure. Putting their findings into practice in a laboratory study, they achieved a much higher than normal rate of egg survival and fertilised 57% by using ICSI (the injection of a single sperm into the egg). This is a figure comparable to fertilisation of fresh oocytes.

The key to their success was to increase the time the oocytes were exposed to the chemical protectants, propanediol plus sucrose, from around 10 minutes to 15 minutes and to double or even triple the normal amount of sugar (sucrose) in the freezing solution.

The main reason that human eggs disintegrate in the freezing process is inadequate dehydration. Longer exposure to the protectant and more help from the extra sucrose meant that the eggs were dehydrated gradually and more completely. This gentler freezing process minimised the formation of ice crystals, which can pierce the egg membrane and kill the cell.

The researchers further established that one factor thought to be important in successful cryopreservation - the presence of the protective nourishing cells surrounding the oocyte (cumulus oophorus) - did not actually have a bearing on success. There was no real difference in survival between eggs frozen with their cumulus oophorus partially removed or totally removed.

But, increasing the exposure time to the protectant and doubling the sucrose solution in the freezin
'"/>

Contact: Margaret Willson
m.willson@mwcommunications.org.uk
44-0-1536-772181
European Society for Human Reproduction and Embryology
26-Feb-2001


Page: 1 2 3 4

Related biology news :

1. Freezing cancer cells makes them prime targets for anti-cancer drug, new study finds
2. Scientists Trace Roots Of Protein That Keeps Antarctic Fish From Freezing
3. Climate change plus human pressure caused large mammal extinctions in late Pleistocene
4. Lycopene slows human prostate tumour growth in mice and combined with vitamin E is even better
5. Most recent common ancestor of all living humans surprisingly recent
6. Wildlife Conservation Society hosts public symposium on human-wildlife diseases
7. Bronfenbrenner book sums up human development
8. No role for simian virus 40 in human pleural mesotheliomas
9. Sugar-coated sea urchin eggs could have sweet implications for human fertility
10. Serotonin metabolites in mollusks suggest pathways for human therapies
11. Wrapping a memory with an experience, capacity for recollection detected in non-human species

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Freezing human eggs new findings point way greater success

(Date:8/20/2014)... years, Virginia Tech,s Wu Feng has built upon a ... "Computing in the Cloud" program, and synergistically complemented it ... from the Air Force on "big computing" for mini-drones ... National Institutes of Health on "big data" for the ... computing" aspects from each grant, he was able to ...
(Date:8/20/2014)... DENVER (August 20, 2014) Women who are poor ... to infants with elevated levels of the stress hormone, ... later in life, according to a new research from ... online recently in the American Journal of Human ... infants and relate it directly to the socioeconomic status ...
(Date:8/20/2014)... grows to about 4,000 members, it triggers an important ... a special type of comb used for rearing male ... Department of Neurobiology and Behaviour at Cornell University, led ... of honeybee colonies. The results are published in Springer,s ... , Reproduction isn,t always a honeybee colony,s top ...
Breaking Biology News(10 mins):The internet was delivered to the masses; parallel computing is not far behind 2The internet was delivered to the masses; parallel computing is not far behind 3The internet was delivered to the masses; parallel computing is not far behind 4CU Denver researcher shows economic disparities impact infant health 2Worker bees 'know' when to invest in their reproductive future 2
(Date:8/20/2014)... 2014 /PRNewswire-iReach/ -- A case study from Intel ... school,s bioengineering department and the Intel® Internet of ... Academic Program, UCSD,s research focuses on exploring the ... Photo - http://photos.prnewswire.com/prnh/20140819/137540 ... of Dr. Todd P. Coleman , Associate ...
(Date:8/19/2014)... Piscataway, NJ (PRWEB) August 20, 2014 ... engineering and regenerative medicine is an inadequate vascular ... engineered constructs and decellularized tissues is critical for ... Researchers have attempted to mitigate deficiencies in vascularization ... including adding scaffold bioactivity, optimizing scaffold design and ...
(Date:8/19/2014)... (PRWEB) August 19, 2014 ... to raise $200,000 to fund a corporate lab ... , This development is a critical component ... of care to the implementation of personalized medicine ... of practicing medicine. , Indiegogo contributions will support ...
(Date:8/19/2014)... 19, 2014 Research and ... the "Spectroscopy Equipment and Accessories - ... their offering. This report ... and Accessories in US$ Thousands by the ... Raman Spectrometers, NMR-EPR Spectrometers, Near Infrared (NIR) ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Vascularization in Tissue Engineering Highlighted at 12th New Jersey Symposium on Biomaterials Science 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3Global Spectroscopy Equipment and Accessories - Strategic Business Report 2014 2
Cached News: