HOME >> BIOLOGY >> NEWS
Gene targeting technique extended to human embryonic stem cells

MADISON - The technique that helped revolutionize modern biology by making the mouse a crucible of genetic manipulation and a window to human disease has been extended to human embryonic stem (ES) cells.

In a study published today (Feb. 10) in the online editions of the journal Nature Biotechnology, a team of scientists from the University of Wisconsin-Madison reports that it has developed methods for recombining segments of DNA within stem cells.

By bringing to bear the technique, known in scientific parlance as homologous recombination, on DNA in human embryonic stem cells, it is now possible to manipulate any part of the human genome to study gene function and mimic human disease in the laboratory dish.

"Indeed, homologous recombination is one of the essential techniques necessary for human ES cells to fulfill their promise as a basic research tool and has important implications for ES cell-based transplantation and gene therapies," write Wisconsin researchers Thomas P. Zwaka and James A, Thomson, the authors of the new study.

The technique has long been used in the mouse and is best known in recent years for its use to generate mice whose genomes have been modified by eliminating one or more genes. Known as 'knockouts,' genetically altered mice have become tremendously important for the study of gene function in mammals, and have been used to explore everything from the underlying mechanisms of obesity and other conditions to the pinpointing of genes that underpin many different diseases.

Significant differences between mouse and human embryonic stem cells have, until now, hampered the application of the technique to human ES cells, according to Zwaka, the lead author of the Nature Biotechnology report and a research scientist working in the laboratory of James Thomson. Thomson was the first to isolate and culture human embryonic stem cells nearly five years ago.

"This is a big benefit for the human ES cell field,"
'"/>

Contact: Thomas P. Zwaka
tzwaka@primate.wisc.edu
608-265-3131
University of Wisconsin-Madison
9-Feb-2003


Page: 1 2 3

Related biology news :

1. Cancer vaccine based on pathogenic listeria bacteria shows promise targeting metastases
2. Sweet success in targeting sugar molecules to cells in living animals
3. Two-pronged attack targeting EGF receptor hinders cancer cell growth
4. Wilex & Fox Chase Cancer Center begin trial w/ novel compound targeting tumor invasion & metastasis
5. Painstaking work targeting TRPV1
6. Male-targeting bacteriums genome is deciphered
7. New Columbia Genomic Center of Excellence aims to speed genetic targeting of medicines
8. Natural fat compound may be basis for new class of drugs targeting obesity
9. UCSF studies illuminate possible new landscape for targeting cancer
10. Pinpoint targeting promises improved success in gene therapy for blood and other disorders
11. ASU researchers demonstrate new technique that improves the power of atomic force micrscopy

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/6/2017)... 5, 2017 RAM Group , ... new breakthrough in biometric authentication based on a ... properties to perform biometric authentication. These new sensors are ... created by Ram Group and its partners. This sensor ... supply chains and security. Ram Group is a ...
(Date:4/17/2017)... , April 17, 2017 NXT-ID, Inc. ... company, announces the filing of its 2016 Annual Report on Form ... Exchange Commission. ... Form 10-K is available in the Investor Relations section of the ... on the SEC,s website at http://www.sec.gov . 2016 ...
(Date:4/11/2017)... Research and Markets has announced the addition of the ... ... to grow at a CAGR of 30.37% during the period 2017-2021. ... been prepared based on an in-depth market analysis with inputs from ... prospects over the coming years. The report also includes a discussion ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... Iowa (PRWEB) , ... October 12, 2017 , ... ... based in Vilnius, Lithuania, announced today that they have entered into a multiyear ... is to provide CRISPR researchers with additional tools for gene editing across all ...
(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... ... Surgical Wound Market with the addition of its newest module, US Hemostats & ... market for thrombin hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic sealants ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... analysis platform specifically designed for life science researchers to analyze and interpret ... Rosalind Franklin, who made a major contribution to the discovery of the ...
(Date:10/11/2017)... ... October 11, 2017 , ... The ... context, enabling overexpression experiments and avoiding the use of exogenous expression plasmids. The ... transformative for performing systematic gain-of-function studies. , This complement to loss-of-function studies, ...
Breaking Biology Technology:
Cached News: