HOME >> BIOLOGY >> NEWS
Genetic trick adapted from viruses makes gene therapy vectors more versatile

A genetic trick used by viruses to replicate themselves has been adapted for laboratory use to build complex protein structures required by immune system cells, according to investigators at St. Jude Children's Research Hospital.

This approach could also be used to develop new gene therapy vectors in cases when cells must be modified to make high levels of different proteins. A vector is a DNA molecule used to ferry specific genes into cells in order to give those cells the ability to make particular proteins.

A report on this work appears in the May 2004 issue of Nature Biotechnology.

The achievement gives researchers a powerful tool for studying the roles of complex proteins in living cells. The study also showed that this technique can reliably produce therapeutically useful amounts of multiple proteins. Some cellular proteins must be present in many copies in order to work efficiently. Because it only borrows a genetic trick from viruses but does not cause a real infection, the technique may increase the usefulness of current gene therapy vectors. Specifically, the technique would permit scientists either to restore complex protein structures that are missing in certain cells or make multiple proteins that act together as potent drugs against cancer and other diseases.

The technique is based on a genetic trick, called a self-cleaving 2A peptide, which is used by some viruses to produce multiple proteins from a single length of DNA; i.e., a single, long protein is produced that automatically breaks into multiple, distinct proteins.

St. Jude researchers used genetically modified mouse immune system cells called T lymphocytes to test the efficiency of this technique in making the CD3 complex, which is part of the T cell receptor, a large protein lodged in the cell's membrane. The receptor allows T cells to "sense" targets that the cells are programmed to destroy. Without the CD3 complex, the T cell receptor is incomplete and canno
'"/>

Contact: Bonnie Cameron
bonnie.cameron@stjude.org
901-495-4815
St. Jude Children's Research Hospital
5-Apr-2004


Page: 1 2 3

Related biology news :

1. Genetic mutations linked to the practice of burning coal in homes in China
2. Genetic differences might help distinguish thyroid cancers
3. Genetic modification of linseed produces healthier omega 3 and 6 fatty acids
4. Wiley publishes Welcome to the Genome: A Users Guide to the Genetic Past, Present, and Future
5. Genetically modified bacterium as remedy for intestinal diseases
6. Genetic analysis rewrites salamanders evolutionary history
7. Genetic map of important tree genes outlined
8. Genetically-engineered marathon mouse keeps on running
9. Genetic clues found for common congenital brain disorder
10. Genetic mutation linked to more aggressive breast cancer found more often in African-Americans
11. Genetic discovery could dramatically reduce need for liver transplants in children

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/14/2018)... ... August 13, 2018 , ... NRD, LLC – NRD® is ... Ionizing Gun. , The ionizing gun features ergonomic design and rugged construction. ... filter and electropolished OSHA-compliant tip. This can be cleanroom packaged (P-2021-5502) or normally ...
(Date:8/14/2018)... ... August 14, 2018 , ... From ... movie “Wonder,” to a better understanding of congenital heart defects, 3-D printing is ... the latest special issue of Birth Defects Research (DOI: 10.1002/bdr2.1367). ...
(Date:8/7/2018)... ... 06, 2018 , ... Nurse practitioners and ... their healthcare careers in the past year, according to the second annual survey ... jobseekers with a wide variety of career- and employment-related resources including physician/candidate database, ...
Breaking Biology News(10 mins):
(Date:7/24/2018)... ... ... R3 Stem Cell is now offering regenerative therapies for COPD lung disease ... Certified providers, with outcomes significantly improving patient quality of life. , Traditional therapies for ... help repair and regenerate damaged lung tissues. Stem cell therapy for COPD ...
(Date:7/24/2018)... ... 24, 2018 , ... On August 6, 2018, BioMedGPS will ... Not just a pretty face, SmartTRAK 3.0’s latest improvements were inspired by its ... world’s first online and real-time data portal that specializes in providing competitive market ...
(Date:7/22/2018)... ... July 19, 2018 , ... Mitotech ... with Essex Bio-Investment for Phase 3 clinical program in Dry Eye Disease. Under ... SkQ1 with approximately $17m allocated towards the first Phase 3 study starting as ...
(Date:7/22/2018)... ... July 20, 2018 , ... ... automation and IT solutions with 150 employees on both the east and west ... Program. Superior Controls met the high criteria for application expertise, operational excellence ...
Breaking Biology Technology:
Cached News: