Genome tools pass big test in fruit flies

s to characterize more than 250 Drosophila genes.

In a research article published in the September, 1999 issue of the journal Genetics, Rubin, Spradling and their colleagues present the results of generating and characterizing 1,052 mutant fly strains that contain P element disruptions in more than 25 percent of Drosophila’s 3,600 vital genes. The researchers examined each fly strain for obvious changes from wild-type flies, including phenotypes such as lethality, near-lethality and other readily apparent physical abnormalities.

"Our goal was not to identify every gene we could, but to show that it was feasible to generate large numbers of P element mutations randomly throughout the Drosophila genome and to use them to find new genes," said Rubin. "We did that." More sophisticated examinations—for behavioral deficits or biochemical changes—should turn up even more genes since only about a third of genes show phenotypes we would have recognized in the current study, he added. Since the Drosophila genome is thought to contain more than 12,000 genes, Rubin and Spradling have concluded that the P element approach, which has been successful in characterizing a large number of fly genes, merits an even larger trial.

Of course, the P element disruption technique is not the only tool that researchers are developing to analyze genomes. In a second research article that will appear in the same issue of Genetics, Rubin, Spradling and more than two dozen colleagues at several institutions in the United States and England, used a wide variety of tools to probe a 2.9 million base pair region of the Drosophila genome.

"We chose a region of the Drosophila genome that had been characterized in substantial detail already from a genetic perspective, partly though the use of P elements, and for which we now have a sequence. The idea, then, was to apply all the available tools to this weal

Contact: Jim Keeley
Howard Hughes Medical Institute

Page: 1 2 3

Related biology news :

1. Wiley publishes Welcome to the Genome: A Users Guide to the Genetic Past, Present, and Future
2. Genome-wide screen reveals new tricks of old genes
3. Genome of first fungal pathogen unveiled
4. Experimental Biology 2004 - Translating the Genome
5. European Virtual Institute for Genome Annotation receives 12 million Euro
6. Tools from the Human Genome Project reveal a versatile microbe
7. Francis Collins and Ari Patrinos receive Energy Secretarys Gold Award for Human Genome Project
8. Genomes of tiny microbes yield clues to global climate change
9. Genome researcher analyze chromosome 7
10. Genome sequence of bread mold revealed by international team, including Hebew University researcher
11. Genome analysis sheds light on drug-resistant pathogen

Post Your Comments:

(Date:6/28/2020)... ... June 25, 2020 , ... ... software solutions for biopharmaceutical R&D, today announced that it has entered into ... full-length multispecific antibodies (Multiclonics®), to support their translational and clinical research strategy ...
(Date:6/25/2020)... ... 2020 , ... eClinical Solutions LLC , a leading ... drug development, is collaborating with Karyopharm Therapeutics (Nasdaq: KPTI ) on randomized ... COVID-19. This is the first study of an XPO1 inhibitor in patients with ...
(Date:6/23/2020)... ... June 23, 2020 , ... First Aid Africa, ... a UK supplier and manufacturer of innovative portable renewable energy equipment, have announced ... fight against the COVID-19 pandemic in Zambia. , One of Renovagen’s FAST FOLD ...
Breaking Biology News(10 mins):
(Date:6/28/2020)... Conn. (PRWEB) , ... June 25, 2020 , ... ... formed to advance photodynamic therapy for treating cancer, today announced the company has ... York (“Roswell Park”). The agreement provides Lumeda globally exclusive rights to Roswell ...
(Date:6/23/2020)... ... 22, 2020 , ... Regen Suppliers, the one stop shop ... three, get the fourth unit free with no limit on the amniotic derived ... exosomes, have been a game changer for regenerative therapies. The products are manufactured ...
(Date:6/11/2020)... ... June 08, 2020 , ... ... delivered through the use of proprietary clean viral vectors, recently announced a key ... States. Greffex’s platform is the world’s most successful gene therapy delivery vehicle ...
(Date:6/3/2020)... ... 03, 2020 , ... Thought Technology Ltd. is offering a ... installment, Dr. Inna Khazan will discuss the benefits of using mindfulness techniques with ... the feelings, thoughts, and emotions they are having in the present moment and ...
Breaking Biology Technology:
Cached News: