HOME >> BIOLOGY >> NEWS
Harvard Medical School Researchers Present Atomic Structure Of DNA-Replicating Enzyme Widely Used In DNA Sequencing

BOSTON--A team of Harvard Medical School researchers have established the 3-D, atom-by-atom structure of a DNA-replicating enzyme at work. This protein, called T7 DNA polymerase, is used in scientific laboratories throughout the world to sequence DNA. This structure will be of special interest to researchers who develop drugs targeting DNA replication. Many antiviral drugs, including the AIDS drug AZT and drugs against herpes simplex virus, inhibit DNA polymerase. In addition, this work may guide the development of better reagents for DNA sequencing--highly sought-after products in this age of the Human Genome Project.

This is the largest protein complex ever to be revealed by the leading method--called MAD--of obtaining high resolution images in X ray crystallography. The structure will be published in the January 15 Nature, along with results from a research collaboration led by crystallographer Thomas Ellenberger and biochemist Charles Richardson, assistant professor and professor, respectively, of biological chemistry and molecular pharmacology at Harvard Medical School.

Other researchers had previously figured out the crystal structure of related enzymes but had never been able to include all the players necessary for DNA duplication. By contrast, this work captures a snapshot of replication at work: the structure shows the polymerase (green, blue, red, salmon-colored areas) bound intimately to a twisted DNA double helix (pink and yellow areas). Where the double helix ends, the DNA continues as a short, single-stranded stretch (yellow), and this is where the polymerase was just about to attach another DNA building block (arrow), when it was frozen in action by the crystallization process.

Moreover, this work for the first time depicts a so-called replicative polymerase. Various types of DNA polymerases occur in every cell of every living thing, but the enzymes crystallized in earl
'"/>

Contact: Bill Schaller
schaller@warren.med.harvard.edu
617-432-0441
Harvard Medical School
15-Jan-1998


Page: 1 2

Related biology news :

1. Harvard Stem Cell Institute hosts inaugural symposia
2. Harvard Medical School launches new department to study human biology at the level of whole systems
3. Harvard Medical School consortium receives grant to harness microbe genomes for environment/energy
4. UCSF-Harvard team publishes major HIV therapy study over objections of sponsor; compound shows no effect on slowing disease progression
5. Harvard Medical School researchers discover first in a new class of mitosis inhibitors
6. Harvard and Alexion researchers report successful transplantation of immuneprotected neurons in primate model of Parkinsons disease
7. Harvard expert speaks on lead toxicity to chemists meeting in New Orleans
8. Harvard Medical School Researchers Identify Four Human Genes Essential To Cell Division; Discovery Yields New Target For Cancer Therapies
9. Harvard, Duke Researchers Discover Off-switch Inside Human Cells
10. Joslin Diabetes Center adds first affiliate in California at Irvine Medical Center
11. LA BioMed Medical/Research Briefs, July/August 2004

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/14/2019)... ... August 13, 2019 , ... Dr. Julie Reck of Veterinary Medical Center of Fort Mill ... Her first stem cell patient, her own geriatric Australian Shepherd, Simon, was treated in June ... wanted to play but his body was weak, and he struggled to play fetch and ...
(Date:8/6/2019)... DURHAM, N.C. (PRWEB) , ... August 06, 2019 ... ... Medicine (SCTM) identifies a computational modeling system that could have far-reaching implications for ... called partial least squares regression (PLSR), is able to predict which stem cell ...
(Date:8/6/2019)... ... 2019 , ... Keck Graduate Institute (KGI) President Sheldon Schuster has announced Martin ... , “Dean Zdanowicz is highly regarded as an innovative leader who prioritizes student success ... great choice to build on the success of the KGI School of Pharmacy and ...
Breaking Biology News(10 mins):
(Date:7/19/2019)... ... July 18, 2019 , ... Global ... to enlarge their current piezo motion facilities in Lederhose, Germany, increasing their current ... multilayer piezo ceramic assembly production in addition to general office space, a significant ...
(Date:7/17/2019)... ... July 16, 2019 , ... ... agreement for the development and manufacturing of Zolgensma® (onasemnogene abeparvovec-xioi), an AveXis ... AveXis, a Novartis company, will have dedicated manufacturing space at the new, ...
(Date:7/9/2019)... ... July 08, 2019 , ... Today, at the ... National Corn Growers Association (NCGA) announced the winners of the Consider Corn ... product or process using field corn to produce biobased materials. , “Corn is a ...
(Date:6/26/2019)... (PRWEB) , ... June 26, 2019 , ... Alice Branton ... treatment on improving physicochemical properties of Magnesium Gluconate, which can prove to be beneficial ... 125% alteration of characteristic differentiation peak, change in crystallite size , Over ...
Breaking Biology Technology:
Cached News: