High-resolution image illuminates catalytic engine of the ribosome

August 11, 2000-Using a high-energy x-ray beam to probe fragile crystals of RNA and protein, researchers have obtained the most detailed images ever seen of the factory where amino acids are linked into chainlike proteins.

The studies illuminate the basic structure of the ribosome, a protein-making machine found in all cells. These insights include the first unequivocal proof that the ribosome is a ribozyme, an RNA enzyme.

In two articles published in the August 11, 2000, Science, researchers led by Thomas A. Steitz, a Howard Hughes Medical Institute investigator at Yale University, report that they have obtained the atomic structure of the 50S subunit of the ribosome at a resolution of 2.4 Ångströms. An Ångström is one ten-billionth (10-10) of a meter.

The ribosome is a large molecular complex of RNA and protein. When ribosomes are isolated from cell extracts, two different fractions are obtained, representing two subunits. The smaller 30S subunit binds the messenger RNA that constitutes the protein's genetic blueprint, as well as the transfer RNA that carries each specific amino acid to be added to the growing chainlike protein molecule. The larger 50S subunit catalyzes the formation of the bond between each amino acid and the growing protein chain.

Steitz and his colleagues at Yale University used the 2.5 billion electron volt x-ray beam at Brookhaven National Laboratory's National Synchrotron Light Source to perform x-ray crystallography on crystals of 50S subunits that were produced with osmium and iridium atoms attached to act as landmarks. Additional data were gathered using the Advanced Photon Source at Argonne National Laboratory.

In x-ray crystallography, protein crystals are bombarded with intense x-ray beams. As the x-rays pass through and bounce off of atoms in the crystal, they leave a diffraction

Contact: Jim Keeley
Howard Hughes Medical Institute

Page: 1 2 3

Related biology news :

1. High resolution satellite imagery assists hunt for infectious kissing bugs
2. Anthrax enzyme images reveal secrets of antibiotic resistance, suggest new drug design
3. Newly designed nanoparticle quantum dots simultaneously target and image prostate tumors in mice
4. Carnegie Mellon neuroscientist develops tool to image brain function at the cellular level
5. New technique images gene expression in mice
6. Molecular image of genotoxin reveals how bacteria damage human DNA
7. St. Jude researchers create image of enzyme that orchestrates natural genetic engineering
8. $9.4 million NSF grant backs UCSB-led effort in bio-image informatics
9. Brain links non-food images with eating -- But only when hungry, study suggests
10. First-ever images of developing dengue virus obtained at Purdue
11. High-speed images show how cells mobilize for immune response

Post Your Comments:

(Date:8/26/2020)... ... August 25, 2020 , ... Modality Solutions, a ... 2020 annual Inc. 5000 list. The list is the most prestigious ranking of the ... well, and for this year’s list, reported three-year revenue growth of 71 percent. ...
(Date:8/21/2020)... ... August 19, 2020 , ... The ... uniting life science, data science, informatics and IT leaders goes VIRTUAL and announces ... of uncertainty and change, Bio-IT takes the leadership role of keeping our life ...
(Date:8/7/2020)... , ... August 06, 2020 , ... ... industry, is pleased to announce that Eric Chen and Jessica Wong have joined ... Product Management & Strategy, respectively. , Eric Chen heads development efforts for Alucio’s ...
Breaking Biology News(10 mins):
(Date:7/22/2020)... ... July 22, 2020 , ... Join experts from Reed Tech , ... Solutions, in a one hour live webinar on Thursday, August 13, 2020 ... for drugs and medical devices. Specifically, for medical devices, the NMPA has departments dealing ...
(Date:7/18/2020)... ... July 16, 2020 , ... Medial EarlySign , ... and prevention of high-burden diseases, and Centric Consulting, a business and technology consulting ... utilize existing data in order to identify and prioritize patients for care. , ...
(Date:7/10/2020)... ... July 09, 2020 , ... ... announced today that Massachusetts Institute of Technology (MIT) has expanded the company’s ... allows PathSensors to move into the point-of-care diagnostic market, focusing initially on ...
(Date:7/7/2020)... ... July 06, 2020 , ... R3 International is now offering stem cell therapy ... stem cells. Depending on the patient's condition, treatment may be offered IV, intrathecal or ... some form of Alzheimers dementia, and the incidence continues to increase as individuals live ...
Breaking Biology Technology:
Cached News: