HOME >> BIOLOGY >> NEWS
How an insidious mutation fools DNA replication

DURHAM, N.C. -- Biochemists have pinpointed how a flaw in DNA that is central to mutations in cancer and aging fools the cellular enzyme that copies DNA. Their finding explains how oxidative DNA damage -- a process long believed to underlie cancers and aging -- can create permanent genetic damage.

The Duke University Medical Center researchers' findings were published online Aug. 22, 2004, by the journal Nature. The scientists were led by Associate Professor of Biochemistry Lorena Beese, Ph.D., and the paper's lead author was Gerald Hsu, a Duke M.D./Ph.D. student. The other co-authors are Thomas Carell and Matthias Ober of Ludwig Maximillians University in Germany. Their research was supported mainly by the National Cancer Institute.

DNA is a double stranded molecule shaped like a spiral staircase. The two strands of the spiral are linked by sequences of molecular subunits, or bases, called nucleotides. The four nucleotides -- guanine, cytosine, adenine and thymine -- naturally complement one another like puzzle pieces. In normal DNA, a guanine matches with a cytosine, and an adenine with a thymine. However, stray reactive oxidizing molecules in the cell can alter guanine to become an "8-oxoguanine" that can lead to a mismatch.

This mismatch occurs in the process of replicating DNA, which begins when the two strands unzip. A protein enzyme called DNA polymerase then works its way along one "template" strand adding nucleotides to create a new double-stranded DNA. In the replication process, the polymerase draws the DNA strand through a small "active site" -- somewhat like a spaghetti strand being drawn through a Cheerio.

Normally, this "high-fidelity" polymerase accurately adds complementary nucleotides and detects any mistakes that have been made. These mistakes or mismatches reveal themselves as malformations that distort the active site -- like kinks in the spaghetti strand that would clog the Cheerio. Such malformations trigger a r
'"/>

Contact: Dennis Meredith
dennis.meredith@duke.edu
919-681-8054
Duke University Medical Center
30-Aug-2004


Page: 1 2 3

Related biology news :

1. Selenium: an insidious and persistent toxin
2. Researchers reveal insidious role of a serpin in Alzheimers Disease
3. Genetic mutations linked to the practice of burning coal in homes in China
4. Spontaneous mutation produces new MAO A/B knockout mouse
5. Using statistics to decipher secrets of natural mutation
6. Rare mutations can significantly increase risk factor for heart disease
7. Genetic mutation linked to more aggressive breast cancer found more often in African-Americans
8. LBP-1a gene mutation linked to disruption of normal fetal development
9. Genetic mutation found that is major contributor to type 1 diabetes
10. The mother lode of mutations
11. Gene mutation causes progressive changes to cell structure in children with Progeria

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/7/2020)... ... August 06, 2020 , ... Nine middle school teachers from ... Program, a unique professional development program that provides advanced STEM (science, technology, engineering ... teachers nationwide. , Created in partnership between the National Stem Cell Foundation and ...
(Date:7/22/2020)... Calif. (PRWEB) , ... July 21, 2020 , ... ... driving digital transformation and innovation in technology and compliance, announces a new solution ... device companies must ensure every layer of their technology stack complies with FDA ...
(Date:7/4/2020)... ... July 03, 2020 , ... Aesthetics Biomedical (ABM), a ... awards for not only the products and treatments developed, but also the dedicated ... RF. All the brands built by ABM have received several honors already for ...
Breaking Biology News(10 mins):
(Date:9/1/2020)... ... September 01, 2020 , ... ... biotechnology, and diagnostics companies, has announced the placement of Julianne Averill , ... responsible for leading all financial operations and implementing key business strategies to accelerate ...
(Date:8/12/2020)... ... August 11, 2020 , ... ... available for free its new white paper – Simulated Transport Methodologies. Regulatory ... today’s biologics licensing application (BLA). In response, Modality Solutions shares an explanation ...
(Date:7/31/2020)... ... ... R3 Stem Cell International is now offering patients the opportunity to receive ... patients may choose which extremities they would like treated. , Several studies have shown ... At R3 International, umbilical cord tissue is obtained from a lab with a long ...
(Date:7/31/2020)... ... , ... eSource has long been touted as the solution to high data ... the reasons it did not take off as quickly as people initially expected, and ... moving towards capturing data electronically for clinical trials and then repurposing it for downstream ...
Breaking Biology Technology:
Cached News: