HOME >> BIOLOGY >> NEWS
Images reveal how rotavirus - leading cause of diarrhea - enters cells

kes," each consisting of a cluster of VP4 molecules. Dormitzer and colleagues trimmed the VP4 protein down to two rocklike sub-components that make up the spike's "head" and "body." They crystallized these pieces and used X-ray diffraction to determine their three-dimensional structures, precise down to the atom. Comparison of the crystal structures to electron microscopy images, obtained by colleagues at the Baylor College of Medicine, suggests that VP4 undergoes two consecutive shape changes that allow it to breach the membrane of the cell it's trying to infect.

"This protein goes through some extraordinary gymnastics that are almost certainly related to its function," says Dormitzer.

First, when rotavirus arrives in the intestine, digestive enzymes cause two of the three VP4 molecules in each cluster to form a rigid spike, priming the virus to attack the cell and positioning the spike "head" to bind to the surface of the target cell. Then, in a second rearrangement, the spikes fold back, and VP4 takes on a folded-umbrella structure with three "panels." Dormitzer and colleagues speculate that this folding motion causes the spike's "body" component to break a hole in the cell membrane, allowing the virus to enter.

The key finding for vaccine development is that the "head" and "body" portions of the VP4 protein contain many of the targets that the immune system recognizes when it attacks the virus and protects against infection.

"The work is a clear example of the way in which structural studies can contribute to new good ideas about strategies for vaccines," says Senior Investigator Stephen Harrison, Ph.D., a Howard Hughes Medical Institute investigator and chief of CHB's Laboratory of Molecular Medicine.

Vaccines made from live viruses (such as RotaShield) or killed viruses can present safety concerns and be unstable without refrigeration. In contrast, the "head" of the VP4 protein is very stable at room temperature
'"/>

Contact: Mary-Ellen Shay
mary.shay@childrens.harvard.edu
617-355-6420
Children's Hospital Boston
25-Aug-2004


Page: 1 2 3

Related biology news :

1. Images of tail of protein needed for cell multiplication suggest anticancer drug targets
2. Images reveal how body regulates salt uptake in cells
3. Images of evolution
4. Images of enzyme suggest way to improve DNA sequencing
5. Laser Microscope At Cornell Images Serotonin In Live Cells
6. DNA lends scientists a hand, revealing new chemical reactions
7. Award winning researchers reveal potential new role for Glivec
8. Hidden diversity: DNA barcoding reveals a common butterfly is actually 10 different species
9. Anthrax enzyme images reveal secrets of antibiotic resistance, suggest new drug design
10. Brown research reveals key insight into memory-making
11. Study reveals why eyes in some paintings seem to follow viewers

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/3/2020)... SAN MATEO, Calif. (PRWEB) , ... September 03, 2020 , ... ... that the Brilliant Smart Home System and its products are now available for purchase ... wholesale distributor of smart home, security, AV, and low-voltage products. In addition to being ...
(Date:9/3/2020)... , ... September 03, 2020 , ... ... data, today announced that an extensive third-party assessment of the company’s security practices ... when measured against federal security regulations. The comprehensive evaluation reviewed Litmus’ processes and ...
(Date:8/26/2020)... , ... August 24, 2020 , ... ... the treatment of chronic inflammatory and fibrotic diseases, today announced that Dr. Stacie ... of Drug Discovery. , Dr. Canan previously served as Executive Director of Global ...
Breaking Biology News(10 mins):
(Date:8/7/2020)... THE WOODLANDS, Texas (PRWEB) , ... August 06, ... ... gene therapy and DNA vaccine industries, announces it has closed on the purchase ... Deison Technology Park in Conroe, TX and includes over 21 acres in the ...
(Date:7/31/2020)... ... 29, 2020 , ... Diversified Technologies, Inc. has introduced a ... to drive Klystrons, TWTs, IOTs, and magnetrons. , DTI Radar Transmitter Systems ... a push-pull configuration; yielding fast fall time for a capacitive load. These all ...
(Date:7/31/2020)... , ... July 30, 2020 ... ... cloud-based software for the life sciences industry, today announced the release of ... designed specifically for Medical Science Liaisons (MSLs) and other field medical professionals. ...
(Date:7/18/2020)... ... 2020 , ... dicentra , a leading Contract Research ... is pleased to announce that Charles Galea has joined its clinical trials division ... an accomplished and results-driven sales executive with over 10 years of expertise in ...
Breaking Biology Technology:
Cached News: