TITLE: Spontaneous and evoked intracellular calcium transients in donor-derived myocytes following intracardiac myoblast transplantation

AUTHOR CONTACT:
Loren J. Field
Indiana University School of Medicine, 1044 West Walnut St, Indianapolis, IN 46202, USA
Phone: 317-274-5085; Fax: 317-274-8679; E-mail: ljfield@iupui.edu

View the PDF of this article at: https://www.the-jci.org/press/21589.pdf

Pathways to Immunity

The presence of bacterial components triggers Toll-like receptors (TLRs) to activate macrophages and DCs through various signal transduction pathways. In response to LPS, a TLR turns on the serine/threonine kinase Cot/Tpl2, which then activates ERK1/2 in macrophages. It remains unknown whether Cot/Tpl2 is involved in responses to other bacterial components or in the activa-tion of other immune cell types. Tetsuya Matsuguchi and colleagues examine the importance of Cot/Tpl2 in response to LPS, synthetic lipopeptide, and bacterial DNA (CpG-DNA) (pages 857866). Using RAW 264.7 cells, the authors showed that all 3 ligands activated Cot/Tpl2. In peritoneal macrophages from Cot/Tpl2/ mice, however, only CpG-DNA could activate ERK, thus it could function through a Cot/Tpl2-independent pathway. Both peritoneal macrophages and immature DCs from the bone marrow of Cot/Tpl2/ mice showed increased IL-12 expression in response to CpG-DNA. Northern blot analysis and gel shift assays demonstrated that enhanced IL-12 levels occurred at least partially through loss of transcriptional repression. In vivo, OVA immunization and Leishmania major infection
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Contact: Laurie Goodman
press_releases@the-jci.org
212-342-4159
Journal of Clinical Investigation
15-Sep-2004