Japanese researchers develop novel method of introducing transgenes into animals

Reproductive biologists at Kyoto University have succeeded in producing the first animal offspring with transgenic material carried directly from sperm stem cells "infected" by a retrovirus. Some of the resulting transgenic mice reproduced and passed on the new genetic material to their offspring.

The team of researchers, headed by Dr. Takashi Shinohara, injected the retrovirus into the seminiferous tubules in the testes of immature male mice. The retrovirus was taken up by the sperm stem cells, which went on to produce sperm with the transgene.

According to Dr. Shinohara and his colleagues Mito Kanatsu-Shinohara and Shinya Toyokuni, 86 percent of the males injected with the retrovirus became fertile. Eight of 31 of these males (26 percent) later mated normally with wild-type female mice and sired offspring. An average of about 3 percent of these offspring had the transgene.

The transgene remained stable and was detected in some offspring in the next generation. When transgenic offspring from injected ("founder') males were mated with wild-type mice, six of eight second-generation offspring from a transgenic male mouse showed the presence of the transgene, as did one of three offspring of a transgenic female mouse.

Because stem cells treated with retroviral transgenes can continuously generate large numbers of transgenic sperm, many transgenic offspring can be produced from a single "founder" male.

In a paper accepted for publication in the journal Biology of Reproduction, the Japanese team notes that other methods have been described for introducing transgenes into animals, including techniques based on eggs or embryos from female animals. The rate of success for these methods, however, is generally less than 1 percent.

The new method of using male sperm stem cells is simple and has a relatively high rate of success. It should be directly applicable for producing a wide range of animals, including pigs and cattle,

Contact: Dr. Takashi Shinohara
Society for the Study of Reproduction

Page: 1 2

Related biology news :

1. Heart disease among some Japanese may be due to sequencing variation inside a gene
2. Japanese shore crabs invade Penobscot Bay, Maine
3. A popular Japanese plum, now available in the US, may help prevent the onset of cardiac disease
4. Press invitation: The Future of Medical Technology: 2001-2010 - Anglo-Japanese Healthcare Conference at Imperial College, London
5. Scholar finds parallels between U.S./Japanese attitudes toward wolves
6. US and Japanese scientists develop new test for male infertility
7. Oliver Smithies wins major award from Japanese research foundation
8. The German-Japanese Consortium deciphers human chromosome 21 - A basis for comprehensive understanding of trisomy 21 (Down syndrome)
9. INEEL microbiologist joins Japanese on expedition to tap marine-methane deposits
10. ASU Professor Helps In Fight To Protect Japanese Pika Habitat
11. Belgian researchers explore revolutionary approach to angiogenesis

Post Your Comments:

(Date:4/6/2017)... 2017 Forecasts by Product Type ... by End-Use (Transportation & Logistics, Government & Public Sector, ... Fossil Generation Facility, Nuclear Power), Industrial, Retail, Business Organisation ... Are you looking for a definitive report on the ... ...
(Date:4/3/2017)... 3, 2017  Data captured by IsoCode, ... detected a statistically significant association between the ... treatment and objective response of cancer patients ... predict whether cancer patients will respond to ... well as to improve both pre-infusion potency testing ...
(Date:3/28/2017)... 2017 The report "Video Surveillance ... Servers, Storage Devices), Software (Video Analytics, VMS), and Service ... Forecast to 2022", published by MarketsandMarkets, the market was ... projected to reach USD 75.64 Billion by 2022, at ... base year considered for the study is 2016 and ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... ... and pregnancy rates in frozen and fresh in vitro fertilization (IVF) ... maternal age to IVF success. , After comparing the results from the fresh ...
(Date:10/10/2017)... ... October 10, 2017 , ... ... advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed licensing rights that give ... Liposomal Nanoparticle), a technology developed in collaboration with Children’s Hospital Los Angeles ...
(Date:10/10/2017)... , Oct. 10, 2017 International research firm Parks ... Strategy, will speak at the TMA 2017 Annual Meeting , October ... trends in the residential home security market and how smart safety and ... Parks ... "The residential ...
(Date:10/10/2017)... ... 10, 2017 , ... The Pittcon Program Committee is pleased ... scientists who have made outstanding contributions to analytical chemistry and applied spectroscopy. Each ... leading conference and exposition for laboratory science, which will be held February 26-March ...
Breaking Biology Technology:
Cached News: