HOME >> BIOLOGY >> NEWS
'Library on a Slide': Whole genome microarrays for comparative genomics

A novel application of microarray technology, where up to 30,000 whole genomes are printed on a single slide, is described in the journal BMC Microbiology this week. The 'Library on a Slide' will help researchers compare the genetic make up of large numbers of bacterial strains to discover which genes are responsible for causing disease.

Even within one species of bacteria, the genetic content can vary by as much as 25% between individual strains. These differences can determine how virulent a particular strain is or which organisms it can colonise. To make significant claims about which genes play a role in which biological processes, researchers must compare large numbers of bacterial genomes and see which genes are associated with a particular trait.

Dr Lixin Zhang and his colleagues from University of Michigan have made this sort of experiment much easier, by developing the 'Library on a Slide' technique. They predict that their high-throughput method will be, "an efficient and cost effective way for sharing and utilizing large strain collections in various comparative genomics studies."

The 'Library on a Slide' is based on current microarray technology the printing and probing of the slide are carried out largely as usual. The most significant difference is that each spot on the slide contains the genomic DNA of one bacterial strain, rather than containing multiple copies of a single gene.

The researchers describe how they adapted a high-throughput method for isolating small DNA fragments to efficiently isolate high quality genomic DNA, and how they optimized the conditions for printing this genomic DNA at high density onto a glass slide. They also tested techniques for identifying individual target genes at high sensitivity using fluorescently labelled probes.

To test the 'Library on a Slide' method the group created a sample array using a collection of E. coli genomes, and probed this to discover which genomes contain
'"/>

Contact: Gemma Bradley
press@biomedcentral.com
44-207-323-0323
BioMed Central
21-Mar-2004


Page: 1 2

Related biology news :

1. Whole body imaging allows better understanding of the immune system
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Keep genome data freely accessible
4. The book opens on the first tree genome
5. NSF awards 22 new projects for plant genome research
6. Milk genome symposium
7. Environmental decontamination, greenhouse gases, and the genome of a methane-loving bacterium
8. Scientists explore genome of methane-breathing microbe
9. National Academies advisory: genome data and bioterrorism
10. Adaptive changes in the genome may provide insight into the genetics of complex disease
11. Complex cells likely arose from combination of bacterial and extreme-microbe genomes

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/2/2016)... Feb. 2, 2016 This BCC Research ... market by reviewing the recent advances in high ... drive the field forward. Includes forecast through 2019. ... the challenges and opportunities that exist in the ... solution developers, as well as IT and bioinformatics ...
(Date:2/2/2016)... NEW YORK , Feb. 2, 2016 Technology ... service presents an analysis of the digital and computed ... Malaysia , and Indonesia ... current trends and market size, as well as regional ... by country and discusses market penetration and market attractiveness, ...
(Date:2/2/2016)... , Feb. 2, 2016  Based on its ... & Sullivan recognizes US-based Intelligent Retinal Imaging Systems ... Sullivan Award for New Product Innovation. IRIS, a ... North America , is poised to ... growing diabetic retinopathy market. The IRIS technology presents ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... -- IsoRay, Inc. (NYSE MKT: ISR), a medical technology company ... for the treatment of prostate, brain, lung, head and ... for the second quarter and six months of fiscal ... --> --> Revenue was $1.19 ... ended December 31, 2015, a 12% increase compared to ...
(Date:2/10/2016)... Springfield, MO (PRWEB) , ... February 10, 2016 ... ... company, will attend the International Society of Pharmaceutical Engineering (ISPE) Rocky Mountain Chapter ... of ISPE is expecting to fill more than 100 tables for its annual ...
(Date:2/10/2016)... , ... February 10, 2016 , ... ... available on Microsoft Azure. On Azure, Arvados provides capabilities for managing and processing ... demand for Microsoft Azure from major institutions collecting and analyzing genomic data,” said ...
(Date:2/9/2016)... ... ... With a presidential election in November and the future of U.S. healthcare in ... top healthcare leaders for a night and day of debates and discussions about what ... the University of Pennsylvania’s Wharton School, will be held February 18 – 19, 2016 ...
Breaking Biology Technology:
Cached News: