HOME >> BIOLOGY >> NEWS
'Library on a Slide': Whole genome microarrays for comparative genomics

A novel application of microarray technology, where up to 30,000 whole genomes are printed on a single slide, is described in the journal BMC Microbiology this week. The 'Library on a Slide' will help researchers compare the genetic make up of large numbers of bacterial strains to discover which genes are responsible for causing disease.

Even within one species of bacteria, the genetic content can vary by as much as 25% between individual strains. These differences can determine how virulent a particular strain is or which organisms it can colonise. To make significant claims about which genes play a role in which biological processes, researchers must compare large numbers of bacterial genomes and see which genes are associated with a particular trait.

Dr Lixin Zhang and his colleagues from University of Michigan have made this sort of experiment much easier, by developing the 'Library on a Slide' technique. They predict that their high-throughput method will be, "an efficient and cost effective way for sharing and utilizing large strain collections in various comparative genomics studies."

The 'Library on a Slide' is based on current microarray technology the printing and probing of the slide are carried out largely as usual. The most significant difference is that each spot on the slide contains the genomic DNA of one bacterial strain, rather than containing multiple copies of a single gene.

The researchers describe how they adapted a high-throughput method for isolating small DNA fragments to efficiently isolate high quality genomic DNA, and how they optimized the conditions for printing this genomic DNA at high density onto a glass slide. They also tested techniques for identifying individual target genes at high sensitivity using fluorescently labelled probes.

To test the 'Library on a Slide' method the group created a sample array using a collection of E. coli genomes, and probed this to discover which genomes contain
'"/>

Contact: Gemma Bradley
press@biomedcentral.com
44-207-323-0323
BioMed Central
21-Mar-2004


Page: 1 2

Related biology news :

1. Whole body imaging allows better understanding of the immune system
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Keep genome data freely accessible
4. The book opens on the first tree genome
5. NSF awards 22 new projects for plant genome research
6. Milk genome symposium
7. Environmental decontamination, greenhouse gases, and the genome of a methane-loving bacterium
8. Scientists explore genome of methane-breathing microbe
9. National Academies advisory: genome data and bioterrorism
10. Adaptive changes in the genome may provide insight into the genetics of complex disease
11. Complex cells likely arose from combination of bacterial and extreme-microbe genomes

Post Your Comments:
(Date:3/30/2015)... Mar. 26, 2015 Research and Markets ... "Global Gesture Recognition Market in Automotive Sector 2014-2018" ... forecast the Global Gesture Recognition market in Automotive Sector ... period 2013-2018. Gesture recognition is the ability ... gestures of an individual. Gesture recognition technology can be ...
(Date:3/24/2015)... PALM BEACH GARDENS, Fla. , March 24, ... of biometric identity management solutions, today announced that ... enabling premium clothing accessory retailer Goorin Bros ... login. The Crossmatch biometrics-based solution secures access to ... customers from the increased risk of data breaches. ...
(Date:3/20/2015)... Mar. 20, 2015 Research and Markets ... of the "India Sensors Market Forecast and ... The sensor market is projected to grow ... Consumer electronics, automotive, industrial and healthcare sectors ... the country. In addition, adoption of MEMS technology ...
Breaking Biology News(10 mins):Global Gesture Recognition Market in Automotive Sector Report 2015 - Growing Number of Partnerships 2Crossmatch Fingerprint Readers Help Retailer Prevent Data Breaches at Point-of-Sale 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 3
(Date:4/20/2015)...   Meditope Biosciences, Inc ., a biotechnology company ... announced presentation of data demonstrating the use of its ... (ADCs). The data were presented in an abstract at the ... Philadelphia . "We ... the many commercial applications that may be possible for ...
(Date:4/20/2015)... Whitehouse Laboratories is pleased and overly excited ... Food and Drug Administration (FDA) inspection and received no ... GMP compliance inspection, as based upon 21CFR Parts 210 ... 6th and was granted NAI (no action indicated) status. ... Good Manufacturing Practice (GMP) quality system required by the ...
(Date:4/17/2015)... Calif. , April 17, 2015 /PRNewswire/ ... received a SBIR grant from the National ... National Institutes of Health (NIH). The grant ... efforts in the development of breakthrough genomic ... using specially fabricated substrates. Centrillion has been ...
(Date:4/17/2015)... April 17, 2015 CIO Review ( ... its 20 Most Promising Pharma and Life Science Technology ... MediSpend global compliance software platform for life sciences companies. ... by a panel of experts and members of CIO ... “MMIS’s MediSpend Platform has been on our radar for ...
Breaking Biology Technology:Meditope Biosciences Presents Data At The American Association Of Cancer Research (AACR) Annual Meeting Validating Its SnAP Technology Platform 2Whitehouse Laboratories Completes FDA Inspection with No Form 483 – No Observations 2Whitehouse Laboratories Completes FDA Inspection with No Form 483 – No Observations 3MMIS Chosen by CIO Review as Top 20 Most Promising Pharma and Life Science Technology Solution Providers 2015 2
Cached News: