Measurement Technique Provides Snapshot Of Cell Physiology

CHAMPAIGN, Ill. -- A new measurement technique that simultaneously can identify and measure more than 30 compounds found in a single cell has been developed by a team of chemists and physiologists at the University of Illinois. The method -- which uses nanoliter sampling, capillary electrophoresis and fluorescence spectroscopy -- is direct, convenient and highly sensitive.

"By combining a nanoliter-volume separation technique with an information-rich spectroscopic detection scheme, we can obtain both qualitative and quantitative chemical information about the target species," said Jonathan Sweedler, a professor of chemistry and a researcher at the university's Beckman Institute for Advanced Science and Technology. "We can therefore more completely identify and measure biologically important compounds in individual cells without performing any chemical reactions to make the compounds detectable."

The measurement technique was developed by Sweedler, graduate research assistant Robert Fuller, physiology professor Rhanor Gillette and visiting scholar Leonid Moroz. The researchers describe the technique in the February issue of the journal Neuron.

"We begin by placing a freshly isolated cell in a microvial where it is homogenized and then drawn into a capillary tube," Fuller said. "The chemicals then separate in the capillary by electrophoresis and move into a flow cell where they are stimulated by a laser. The laser-induced fluorescence is then collected by a CCD/spectrograph and analyzed by a computer.

"We can identify compounds not only by the separation time, but also by the spectral fingerprints in the fluorescence emission," Fuller said. "This means we are able to distinguish between compounds that migrate at the same time, thereby avoiding potential misidentification."

In addition, because the detection scheme is based upon the native fluorescence of individual molecules, the researchers need not perform any additional

Contact: James E. Kloeppel, Physical Sciences Editor
University of Illinois at Urbana-Champaign

Page: 1 2

Related biology news :

1. Measurement clarifies role between protein structure and cell adhesion
2. West Coast Measurements Confirm Asian Air Pollution Can Travel To U.S.
3. Measurements Of Protein Surface Verify Electrostatics Model
4. Fast Measurement Technique Reveals Early Steps In Protein Folding
5. Nerve cells guided to repair spinal damage: Technique
6. Technique plucks rapidly evolving genes from a pathogens genome
7. Technique kills cancerous cells, leaves healthy cells intact
8. Technique may identify novel disease genes at a faster clip
9. Technique brings immune-based therapies closer to reality
10. Technique to induce cancer cells to commit suicide developed by Hebrew University scientists
11. UF Anthropologists Study Techniques To Solve Mysteries Of Dead Bodies

Post Your Comments:

(Date:8/5/2020)... ... , ... Regenative Labs has received approval from the Centers for Medicare & ... first Wharton’s jelly allografts to be assigned a Q code and be approved for ... Wharton’s jelly allograft product to be recognized as a 361 HCT/P by CMS regulated ...
(Date:7/18/2020)... ... July 16, 2020 , ... A study has been published ... Neoasis™ active noise control device to attenuate typical noises in a simulated neonatal ... the alarm sounds from patient monitors, ventilators and other bedside devices that would ...
(Date:7/2/2020)... ... July 01, 2020 , ... AltruBio Inc. http://www.altrubio.com ... a biologic for the treatment of steroid-refractory acute graft-versus-host disease (SR-aGVHD), at European ... J Martin of the Fred Hutchinson Cancer Research Center, this single-arm, open-label Phase ...
Breaking Biology News(10 mins):
(Date:8/12/2020)... FARMS, Mich. (PRWEB) , ... August 12, 2020 ... ... Consulting Group, Inc. (EMMA International), a global leader in FDA compliance consulting ... of the nation’s fastest-growing private companies. The list represents a unique look at ...
(Date:8/3/2020)... (PRWEB) , ... August 03, 2020 , ... ... collection, today announced Jim Corrigan, President and CEO has been named one of ... broad cross-section of industry sectors, PharmaVoice 100 honorees are selected based on how ...
(Date:7/31/2020)... ... July 29, 2020 , ... R3 Stem Cell International is ... $3950. With 50 million stem cells total, patients may choose which extremities they would ... for arthritic joints (BMC Musculoskelet Disord. 2016). At R3 International, umbilical cord tissue is ...
(Date:7/31/2020)... ... July 29, 2020 , ... ... transmitter systems that can be configured to drive Klystrons, TWTs, IOTs, and magnetrons. ... drive one or two switches in a push-pull configuration; yielding fast fall time ...
Breaking Biology Technology:
Cached News: