CHAMPAIGN, Ill. - Scientists studying the adhesive properties of cadherin - a protein that binds cells into soft tissue - have found a built-in safety mechanism that may keep cells from ripping apart. "Cadherins hold soft tissues together by binding to identical proteins on adjacent cell surfaces," said Deborah Leckband, a professor of chemical engineering at the University of Illinois. "The resulting strong, intercellular junctions play an important role in holding our tissues together. The proteins are also important in facilitating the cell-sorting process during embryonic development."
Because there are a number of diseases "associated with malfunctions or mutations of the cadherin protein," Leckband said, "a better understanding of how these molecules bind to one another may eventually allow the use of gene therapy to devise potential cures." To study the adhesive properties of cadherin, Leckband and her colleagues used a surface-force apparatus to measure the molecular forces between two cadherin monolayers as a function of the distance between them. Leckband's colleagues were graduate student Sanjeeve Sivasankar and postdoctoral research associate Nicolay Lavrik at the U. of I., and graduate student Bill Brieher and molecular biologist Barry Gumbiner at the Memorial Sloan-Kettering Cancer Center in New York City.
"Our direct-force measurements show that the current model for cadherin is insufficient to explain how this protein binds to its neighbors," Leckband said. "We found that the proteins adhere strongest when they overlap completely; not when just their ends touch, as the model had suggested. In fact, our measurements indicate that the ends of the proteins contribute very little to the binding process."
One of the scientists' most surprising findings, however, was the unusual way in
which the proteins detached from one another. "Instead of simply snapping
apart, the cadherins first moved apart slowly, then built up speed and finall
Contact: James E. Kloeppel, Physical Sciences Editor
University of Illinois at Urbana-Champaign