HOME >> BIOLOGY >> NEWS
Microarrays, key genome expression trackers, work better when probes are sequence-verified

BETHESDA, Md. (July 22, 2004) -- Microarray technology, sometimes referred to as biochips, has been extensively used to investigate genome-wide expression patterns and has facilitated a revolution in the characterization of cellular regulation. In addition, comprehensive gene expression profiling shows great potential for human disease diagnostics.

For instance, multiple research groups have shown that microarray data can identify previously unappreciated molecular subtypes of lung cancer that differ in their prognoses. Unfortunately, poor reproducibility of results exists across studies.

Furthermore, there is now a tremendous volume of data, particularly from human clinical specimens, which can't be duplicated, so strategies to improve analysis of (that is, "clean up") existing data sets are needed. One limitation of the application of microarray technology could be due to the failure of similar studies to measure identical biological parameters. In other words, the problem could arise from the fact that many of the microarray probes and there are now up to hundreds of thousands on a single slide are often based on gene sequences that are five years old, or more.

Background

Frustrated by more than two years of trying to analyze microarray data contrasting two known conditions, researchers at Harvard Medical School and Washington University in St. Louis decided to look at the nucleotide sequences that measure gene expression on the most widely used commercial microarray technology. They found that in many cases they did not match the most current information.

In this study, they undertook a global analysis of the microarrays and systematically attempted to confirm the accuracy of individual probe sequences. They looked at every probe on the array to see if it corresponded with the gene that it was intended to measure. They found that an important percentage of the probe sequences -- sometimes as much as 20%, on both
'"/>

Contact: Mayer Resnick
mresnick@the-aps.org
301-634-7209
American Physiological Society
22-Jul-2004


Page: 1 2 3

Related biology news :

1. Scientists sequence genome of kind of organism central to biospheres carbon cycle
2. Keep genome data freely accessible
3. The book opens on the first tree genome
4. NSF awards 22 new projects for plant genome research
5. Milk genome symposium
6. Environmental decontamination, greenhouse gases, and the genome of a methane-loving bacterium
7. Scientists explore genome of methane-breathing microbe
8. National Academies advisory: genome data and bioterrorism
9. Adaptive changes in the genome may provide insight into the genetics of complex disease
10. Complex cells likely arose from combination of bacterial and extreme-microbe genomes
11. Researchers identify the genomes controlling elements

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/3/2017)... 2017  Data captured by IsoCode, IsoPlexis ... a statistically significant association between the potency ... and objective response of cancer patients post-treatment. ... whether cancer patients will respond to CAR-T ... as to improve both pre-infusion potency testing and ...
(Date:3/28/2017)... , March 28, 2017 ... Biometrics), Hardware (Camera, Monitors, Servers, Storage Devices), Software (Video ... and Region - Global Forecast to 2022", published by ... in 2016 and is projected to reach USD 75.64 ... 2017 and 2022. The base year considered for the ...
(Date:3/23/2017)... Research and Markets has announced the addition ... - Industry Forecast to 2025" report to their offering. ... The Global Vehicle ... around 8.8% over the next decade to reach approximately $14.21 billion ... estimates and forecasts for all the given segments on global as ...
Breaking Biology News(10 mins):
(Date:8/18/2017)... ... August 18, 2017 , ... Producers of the ... Hybrids, Inc. in an upcoming episode, scheduled to broadcast fourth quarter 2017. American ... Federal Hybrids, the independent, family-owned seed company. Educating audiences about its broad portfolio ...
(Date:8/16/2017)... ... August 16, 2017 , ... Nanoscience ... Electrospraying line of nanofiber and nanoparticle fabrication instruments from Bioinicia. The ... fully automated pilot plants and equipment for industrial manufacturing. All equipment is ...
(Date:8/16/2017)... 16, 2017  This year,s edition of the Inc. 5000 features a ... workforce solutions, has made the list for the third year in a ... fastest-growing private companies based on a set of quantitative metrics. In addition, ... fastest-growing companies in the Bay State . ... Inc. 5000 ...
(Date:8/15/2017)... Pittsburgh, PA (PRWEB) , ... August 15, 2017 ... ... JASIS, one of the largest Asian exhibitions for analytical and scientific instruments. This ... Pittsburgh), will be “New Approaches in Mass Spectrometry for Bioanalytical Applications.” This dynamic ...
Breaking Biology Technology:
Cached News: