HOME >> BIOLOGY >> NEWS
Molecule that 'blocks' key bacterial enzyme may lead to new antibiotics

NEW BRUNSWICK/PISCATAWAY , N.J. Rutgers scientists have deciphered the complex mechanics of microcin J25 (MccJ25), a tiny, natural molecule that acts like a cork in a bottle to block a key bacterial enzyme potentially leading to a new generation of antibiotics.

Two teams of researchers at Rutgers, The State University of New Jersey, discovered independently that MccJ25 uniquely blocks a "tunnel" into the bacterial enzyme, RNA polymerase (RNAP). The "tunnel" is used to bring raw materials for RNA synthesis into the enzyme and to expel byproducts of RNA synthesis.

"Closing the crowded, two-way 'tunnel' starves RNAP, shuts it down and kills the bacteria," said Richard H. Ebright, a Howard Hughes Medical Institute investigator, and a professor in Rutgers' department of chemistry and chemical biology and the Waksman Institute of Microbiology. "Understanding the way in which MccJ25 works sets the stage for the development of novel antibacterial drug designs."

To understand how MccJ25 works, Ebright's group used genetic methods to test hundreds of thousands of RNAP derivatives, or variants, in order to define the binding sites for MccJ25 on RNAP. The researchers also used biophysical methods, attaching fluorescent tags to MccJ25 and to each of a dozen sites in RNAP. Using the tags, the researchers gauged the position of each bound pair in a GPS-like manner, verifying the results of the genetic work. They then used biochemical methods to find out what happened once MccJ25 binds to the RNAP.

The research team of Konstantin Severinov, an associate professor in Rutgers' department of molecular biology and biochemistry and the Waksman Institute, had been the first to demonstrate that RNAP from cells resistant to MccJ25 also showed resistance to the drug in a test tube. In their current work, these researchers used biochemical methods to characterize, in molecular detail, the mechanism of MccJ25 action. In
'"/>

Contact: Joseph Blumberg
blumberg@ur.rutgers.edu
732-932-7084 x652
Rutgers, the State University of New Jersey
17-Jun-2004


Page: 1 2

Related biology news :

1. Molecule awakens and maintains neural connections
2. Molecule found to be critical for kidney development
3. Molecule identified that contributes to essential cell functioning process
4. Molecules discovered that extend life in yeast, human cells
5. Molecule helps prevent inflammation and heart disease in fat-fed mice
6. Molecule offers insights into fertilization
7. Molecules designed to interfere with DNA upon signal
8. Molecule helps pupils respond to light
9. Molecule that restricts mouse cells potency could yield embryonic stem cells without the sacrifice of embryos
10. Molecule plays key role in cellular quality control machinery
11. Scientists Determine Atomic Structure Of Lego-Like Molecule That Self-Assembles Into Cells Cargo Capsule

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/14/2017)... (NYSE: IBM ) is introducing several innovative partner startups ... collaboration between startups and global businesses, taking place in ... nine startups will showcase the solutions they have built with ... France is one of the ... percent increase in the number of startups created between 2012 ...
(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... platform specifically designed for life science researchers to analyze and interpret datasets, ... Franklin, who made a major contribution to the discovery of the double-helix ...
(Date:10/11/2017)... ... ... Personal eye wash is a basic first aid supply for any work environment, but most ... you rinse first if a dangerous substance enters both eyes? It’s one less decision, and ... unique dual eye piece. , “Whether its dirt and debris, or an acid or alkali, ...
(Date:10/11/2017)... , Oct. 11, 2017  VMS BioMarketing, a leading ... a nationwide oncology Clinical Nurse Educator (CNE) network, which will ... need for communication among health care professionals to enhance the ... nurses, office staff, and other health care professionals to help ... breast cancer. ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... implantation and pregnancy rates in frozen and fresh in vitro fertilization (IVF) ... and maternal age to IVF success. , After comparing the results from the ...
Breaking Biology Technology:
Cached News: