HOME >> BIOLOGY >> NEWS
Nanotech instruments allow first direct observations of RNA 'proofreading'

When Ralph Waldo Emerson said that nature pardons no mistakes, he wasn't thinking about RNA polymerase (RNAP) - the versatile enzyme that copies genes from DNA onto strands of RNA, which then serve as templates for all of the proteins that make life possible.

Emerson's comment notwithstanding, RNAP makes plenty of mistakes but also proofreads and corrects them before they have a chance to create abnormal proteins. The error-prone nature of RNAP is not surprising given the size of its task. In human cells, for example, the RNAP enzyme has to make precise genetic copies from a DNA double helix that consists of billions of chemical bases known as A, T, G and C. It works like this: After latching onto the double helix, RNAP pulls it apart and starts building new RNA molecules by copying one DNA base at a time.

With thousands of A's, T's, G's and C's to transcribe, RNAP sometimes gets confused and copies the wrong base. Such errors occur roughly once every 1,000 bases, but RNAP's remarkable self-correcting mechanism manages to catch most of them.

''If the error is allowed to propagate, it could result in a bad protein or a wrong protein, but RNAP is an incredibly smart enzyme,'' says Steven M. Block, a professor of biological sciences and of applied physics at Stanford University. ''When RNAP adds the wrong base, it backs up on the DNA helix a little bit, cleaves off the piece of RNA that has the bad base in it and starts up again. That's the hypothesis, at least.''

New experiments

In a new study in the journal Nature, Block and his colleagues present strong evidence to support this proofreading hypothesis. Their results - based on actual observations of individual molecules of RNAP - are posted on Nature's website: http://www.nature.com. In another set of experiments published in the Nov. 14 issue of Cell magazine, the researchers discovered that RNAP makes thousands of brief pause
'"/>

Contact: Mark Shwartz
mshwartz@stanford.edu
650-723-9296
Stanford University
25-Nov-2003


Page: 1 2 3 4

Related biology news :

1. National Academies Advisory: Sept. 18-19 Nanotechnology Conference
2. Nanotechnology pioneer slays grey goo myths
3. Nanotechnology highlights from the ACS national meeting in Anaheim, Calif., March 28-April 1
4. Nanotechnology: sink or swim?
5. Nanotech strategy could create new organs
6. Nanotech decoys for viruses
7. NCAR aircraft, ground instruments to track carbon dioxide uptake
8. Acoustic technique could disinfect medical instruments without heat or harsh chemicals
9. ORNL conducting field test of instruments to detect lead
10. Computer database being developed at Temple will allow for better inventory of chemicals
11. Gene duplication allowed pigs to have more babies

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/5/2017)... April 4, 2017 KEY FINDINGS ... expand at a CAGR of 25.76% during the forecast ... the primary factor for the growth of the stem ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem cell ... application, and geography. The stem cell market of the ...
(Date:4/3/2017)... , April 3, 2017  Data captured ... engineering platform, detected a statistically significant association ... prior to treatment and objective response of ... potential to predict whether cancer patients will ... treatment, as well as to improve both pre-infusion ...
(Date:3/30/2017)... HONG KONG , March 30, 2017 ... developed a system for three-dimensional (3D) fingerprint identification by adopting ground ... technology into a new realm of speed and accuracy for use ... applications at an affordable cost. ... ...
Breaking Biology News(10 mins):
(Date:7/15/2017)... ... July 15, 2017 , ... Cuvette manufacturer FireflySci has been making ... the people at FFS have learned that their biggest asset was their amazing customer ... to launch new products to meet the changing needs of scientists at a rapid ...
(Date:7/14/2017)... Liege, Belgium/ Denville, USA/ Martinsried, Germany (PRWEB) , ... ... ... today announced a newly developed standardized solution for sample preparation of proteins to ... Kit) and the Diagenode Bioruptor® sonication system. , The PreOmics iST Kit ...
(Date:7/13/2017)... ... July 13, 2017 , ... FireflySci Inc. started manufacturing ... forward seven years and now they are home to a tremendous line of ... accuracy, and resolution testing. , One mega advantage that FireflySci brings ...
(Date:7/13/2017)... ... 13, 2017 , ... Microscan , the number-one brand of embedded clinical ... advancements of the MicroHAWK platform for barcode reading and machine vision, LVS ... Clinical Lab Expo, taking place on August 1–3 in San Diego, CA. , ...
Breaking Biology Technology:
Cached News: