HOME >> BIOLOGY >> NEWS
New Fluorescence Technique For Ultra-Sensitive Enzyme Characterization Developed - Screening Catalytic Activity At The Single-Molecule Level

New fluorescence technique for ultra-sensitive enzyme characterization developed - Screening catalytic activity at the single-molecule level

Enzymes are characterized by and valued for their ability to catalyze a wide range of chemical reactions. Consequently, precise analytical techniques for monitoring enzyme functions are indispensable tools for the understanding of biological phenomena at the molecular level, and for the development of new pharmaceuticals. A research team at the Max Planck Institute for Biophysical Chemistry in Gttingen, Germany, headed by the Nobel laureate Manfred Eigen, published two articles in PNAS (Proceedings of theNational Academy of Sciences) introducing dual-color fluorescence cross-correlation spectroscopy as a new method for an ultra-sensitive characterization of enzyme activity (PNAS 95:1416-1420, abstract: http://www.pnas.org/cgi/content/abstract/95/4/1416) and demonstrating its potential for high throughput screening (PNAS 95:1421-1426, abstract: http://www.pnas.org/cgi/content/abstract/95/4/1421).

In recent years, fluorescence correlation spectroscopy (FCS) has become an attractive analytical tool for the investigation of biomolecular processes. Due to the interplay of modern confocal optics, new dyes as efficient fluorescent probes, sensitive photodetectors, and fast data processing, FCS allows the observation of the dynamics of single molecules in real time while they pass an open volume element of about one femtoliter, i.e. only the size of a common bacterial cell. This method was invented and worked out by groups at the Cornell University, Ithaca, at the Karolinska Institute, Stockholm, and at the Max Planck Institute, Gttingen; nowadays it has
'"/>

Contact: Ulrich Kettling, Andre Koltermann
ukettli@gwdg.de, akolter@gwdg.de
+49-551-201-1682
Max-Planck-Gesellschaft
12-Mar-1998


Page: 1 2 3

Related biology news :

1. Nerve cells guided to repair spinal damage: Technique
2. Technique plucks rapidly evolving genes from a pathogens genome
3. Technique kills cancerous cells, leaves healthy cells intact
4. Technique may identify novel disease genes at a faster clip
5. Technique brings immune-based therapies closer to reality
6. Technique to induce cancer cells to commit suicide developed by Hebrew University scientists
7. UF Anthropologists Study Techniques To Solve Mysteries Of Dead Bodies
8. Penn Researchers Develop Gene Therapy Technique That Reverses Muscle Membrane Weakness In Muscular Dystrophy Variant
9. Researchers Uncover 3-D Structure Of Virus Replication Technique
10. UF Researcher: New Techniques Help Reconstruct Ancient Diets
11. Technique Measures Muscle Contraction At Molecular Level

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/2/2020)... ... July 01, 2020 , ... AltruBio Inc. http://www.altrubio.com ... a biologic for the treatment of steroid-refractory acute graft-versus-host disease (SR-aGVHD), at European ... J Martin of the Fred Hutchinson Cancer Research Center, this single-arm, open-label Phase ...
(Date:6/28/2020)... Fla. (PRWEB) , ... June 25, 2020 , ... In ... focus on innovations in accelerated Point of Care Testing solutions (POCT). Check local listings ... Samples are collected at medical offices and are shipped to labs throughout the country. ...
(Date:6/23/2020)... ... 23, 2020 , ... Rigaku Corporation , a global ... a series of TOPIQ webinars, which will cover the Transferable Aspherical Atom Model ... response to social distancing measures that resulted in postponements and cancelations of global ...
Breaking Biology News(10 mins):
(Date:7/18/2020)... ... July 17, 2020 , ... ... firm for the life sciences and food industries, is pleased to announce that ... Clinical Research – Business Development. , Charles is an accomplished and results-driven sales ...
(Date:7/10/2020)... ... July 09, 2020 , ... ... Technology (MIT) has expanded the company’s exclusive license to include clinical applications ... point-of-care diagnostic market, focusing initially on the SARS-CoV-2 biosensor. CANARY’s™ fast ...
(Date:7/10/2020)... , ... July 09, 2020 , ... ... cell therapy, today announced the hiring of Allen R. Nissenson, M.D., F.A.C.P., as ... oversee the clinical development of Sentien’s lead product, SBI-101. Dr. Nissenson serves ...
(Date:7/7/2020)... , ... July 06, 2020 , ... ... Awards. Entries from Roche, Eli Lilly, Bristol-Myers Squibb, the University of Chicago, Massachusetts ... Bio-IT World has hosted an elite awards program, highlighting outstanding examples of how ...
Breaking Biology Technology:
Cached News: