New Gene Therapy Technique Results In Efficient Gene Delivery

BOSTON, Mass., June 5--Viruses are normally all together too good at entering our cells. But when scientists alter them to carry desirable genes for genetic therapy, they usually have difficulty getting into the cells where their cargo is needed. Harvard Medical School researchers seem to have overcome this obstacle by building these weighty, less pugnacious viruses a bridge.

The new technique links the virus and target cells by a bridge consisting of two proteins?the growth factor EGF and the receptor for a protein found on the avian leukosis virus (ALV). When applied to cultured cells, the protein bridge allowed the virus not only to bind but also enter the designated cell targets. The researchers report these findings in the June 9 Proceedings of the National Academy of Sciences.

"The novel thing here is that this delivery device works efficiently, which allows for the first time the possibility that we can use this approach to target any cell type we want," says John Young, associate professor of microbiology and molecular genetics. He developed the method in collaboration with graduate student Sophie Snitkovsky.

Most efforts to design in vivo gene delivery systems have focused on further modifying the virus, for example, by building into its envelope a structure that directs the virus to a specific cell type. But the tinkering appears to interfere with the ability of the virus to enter the cells.

"We wanted to find an approach where we could preserve as much as possible the normal virus envelope -cell receptor interaction," says Young. He and Snitkovsky created the EGF-ALV receptor protein bridge and applied it to a series of cell types, including one with the normal EGF receptor and one without. Only those cells with the EGF receptor bound the molecular bridge.

To see if the virus, once linked, could actually enter cells, the researchers introduced a gene conferring resistance to the antibiotic neomyc

Contact: Peta Gillyatt
Harvard Medical School

Page: 1 2

Related biology news :

1. 1st International Conference on Cell Therapy for Cardiovascular Disease
2. National Cancer Gene Therapy Foundation gives $4.0 million in research grants
3. Braunschweig Prize 2003 for Molecular Cancer Therapy
4. Alliance for Cancer Gene Therapy announces $1.5 million in national grants to young investigators
5. New research on safety of intracoronary delivery of adenovector reported in cover story of Molecular Therapy
6. Therapy hinders AIDS virus from evolving drug resistance, Hopkins researchers find
7. Invitation To Cover: American Society Of Gene Therapy Meeting
8. Gene Therapy For Pain
9. Role Of Protein Linked To Colon Cancer Identified, Offering Potential Target For Therapy
10. Shock Therapy Exceeds Expectations In Cleaning Up Contaminated Soils
11. Penn Researchers Develop Gene Therapy Technique That Reverses Muscle Membrane Weakness In Muscular Dystrophy Variant

Post Your Comments:

(Date:8/12/2020)... ... August 11, 2020 , ... ... Princeton, NJ, have entered into license agreements with Housey Pharma’s HMI subsidiary to ... Roche and J&J have annual Research and Development spending in excess of US ...
(Date:8/7/2020)... ... August 06, 2020 , ... Alucio™, a fast-growing ... that Eric Chen and Jessica Wong have joined the company’s leadership team as ... , Eric Chen heads development efforts for Alucio’s flagship product, Beacon, an innovative ...
(Date:8/5/2020)... Fla. (PRWEB) , ... August 05, 2020 , ... Regenative ... cover two new innovations, CoreText™ and ProText™, making them the first Wharton’s jelly allografts ... defect using a syringe. The company’s solutions are the first Wharton’s jelly allograft product ...
Breaking Biology News(10 mins):
(Date:8/3/2020)... LEXINGTON, Ky. (PRWEB) , ... August 03, 2020 , ... ... innovative products for transformative growth, , Known as MediVet Biologics ... Health brand. , The new Ardent Animal Health will build on its ...
(Date:7/31/2020)... and SOMERSET, N.J (PRWEB) , ... July 29, ... ... genome editing company, and Catalent, the leading global provider of advanced delivery technologies, ... health products, today announced that they have entered into a strategic partnership whereby ...
(Date:7/18/2020)... ... ... After research model surgery and catheter implantation, the long journey of catheter maintenance ... a successful study, while protecting and accessing the catheter or device post-surgery is often ... Envigo in a live webinar on Wednesday, August 5, 2020 ...
(Date:7/10/2020)... ... July 09, 2020 , ... ... of Technology (MIT) has expanded the company’s exclusive license to include clinical ... the point-of-care diagnostic market, focusing initially on the SARS-CoV-2 biosensor. CANARY’s™ ...
Breaking Biology Technology:
Cached News: