HOME >> BIOLOGY >> NEWS
New dye directly reveals activated proteins in living cells

CHAPEL HILL -- A series of experiments reported on this week in the journal Science shows for the first time that novel biosensor dyes can directly reveal activation of proteins in individual living cells.

The research, led by Dr. Klaus M. Hahn, professor of pharmacology at the University of North Carolina at Chapel Hill's School of Medicine, demonstrated that at least one of the dyes Hahn developed makes it possible to dramatically visualize the changing activation and intracellular location of the protein Cdc42.

The novel dyes open new possibilities for screening the molecular effects of drugs within the living cell. Currently, automated "high throughput" drug assays are conducted on thousands of cells at a time, but in vitro, in laboratory test tubes.

Cdc42, a member of the Rho family of proteins, regulates multiple and sometimes opposite functions within the cell: movement, proliferation, cell death and shape.

Injected into connective tissue cells, the dye "I-SO" displayed a bright green-colored fluorescence as Cdc42 activation and interaction with other proteins occurred. In addition, the dye proved highly sensitive, enabling detection of protein activation at low levels, unlike current fluorescence methods that require protein over-expression for detection.

"For the first time we saw native Cdc42 activity in living cells," Hahn said. "But perhaps the most important aspect of the paper is that we demonstrated a new approach: We showed we can look at endogenous molecules and their activation using novel dyes."

Unlike other protein visualization methods, "you're looking directly at the fluorescence from this dye, which means it's much brighter and more sensitive," Hahn said.

Also differing from current methods, the new approach does not require making modifications to the protein in question.

"Many proteins occur in small amounts, so if you put in exogenous material you change everything," Hahn said.
'"/>

Contact: L. H. Lang
llang@med.unc.edu
919-843-9687
University of North Carolina School of Medicine
10-Sep-2004


Page: 1 2

Related biology news :

1. MRI technique lets researchers directly compare monkey and human brains
2. Discovery sheds light on mechanisms that allow cells to steer directly toward chemical cues
3. Immune molecule found to kill indirectly by closing receptor to live-saving help
4. Hidden diversity: DNA barcoding reveals a common butterfly is actually 10 different species
5. Brown research reveals key insight into memory-making
6. Study reveals why eyes in some paintings seem to follow viewers
7. Study reveals first genetic step necessary for prostate cancer growth
8. Study of flu patients reveals virus outsmarting key drug
9. Study in Science reveals recreational fishing takes big bite of ocean catch
10. Plumbing trees plumbing reveals their engineering skill
11. New survey reveals insights into unique relationship between mothers and pediatricians

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/30/2017)... Trends, opportunities and forecast in this market ... (fingerprint, AFIS, iris recognition, facial recognition, hand geometry, vein ... use industry (government and law enforcement, commercial and retail, ... others), and by region ( North America ... Pacific , and the Rest of the World) ...
(Date:3/24/2017)... The Controller General of Immigration from Maldives Mr. Mohamed Anwar ... the prestigious international IAIR Award for the most innovative high security ePassport ... ... Maldives Immigration Controller General, Mr. ... on the right) have received the IAIR award for the "Most innovative ...
(Date:3/22/2017)... 2017   Neurotechnology , a provider of ... announced the release of the SentiVeillance 6.0 ... facial recognition using up to 10 surveillance, security ... The new version uses deep neural-network-based facial detection ... utilizes a Graphing Processing Unit (GPU) for enhanced ...
Breaking Biology News(10 mins):
(Date:4/21/2017)... (PRWEB) , ... April 21, 2017 , ... The AMA ... 11 high school graduates from across the nation. The scholarships are created through funds ... member dues. , Scholarship criteria are set by the AMA Scholarship Committee, which is ...
(Date:4/20/2017)... Ca (PRWEB) , ... April 20, 2017 , ... ... Virtual Event , this new webinar will explore challenging patient cases when screening ... to the hospital, there may be a need for bridging parental anticoagulation especially ...
(Date:4/20/2017)... SANTA BARBARA, CALIFORNIA (PRWEB) , ... April 20, 2017 , ... ... process optimization firm for the life sciences and healthcare industries, is pleased to announce ... of the new established USDM subsidiary “USDM Europe GmbH” based in Germany. , ...
(Date:4/20/2017)... LAVAL, QC , April 20, 2017 /PRNewswire/ - Prometic Life ... today presented new results at the International Liver Congress ("ILC") ... Liver ("EASL") in Amsterdam on the ... in a mouse model of obesity and metabolic syndrome. ... According to Dr. Lyne Gagnon, ...
Breaking Biology Technology:
Cached News: