HOME >> BIOLOGY >> NEWS
New dye directly reveals activated proteins in living cells

CHAPEL HILL -- A series of experiments reported on this week in the journal Science shows for the first time that novel biosensor dyes can directly reveal activation of proteins in individual living cells.

The research, led by Dr. Klaus M. Hahn, professor of pharmacology at the University of North Carolina at Chapel Hill's School of Medicine, demonstrated that at least one of the dyes Hahn developed makes it possible to dramatically visualize the changing activation and intracellular location of the protein Cdc42.

The novel dyes open new possibilities for screening the molecular effects of drugs within the living cell. Currently, automated "high throughput" drug assays are conducted on thousands of cells at a time, but in vitro, in laboratory test tubes.

Cdc42, a member of the Rho family of proteins, regulates multiple and sometimes opposite functions within the cell: movement, proliferation, cell death and shape.

Injected into connective tissue cells, the dye "I-SO" displayed a bright green-colored fluorescence as Cdc42 activation and interaction with other proteins occurred. In addition, the dye proved highly sensitive, enabling detection of protein activation at low levels, unlike current fluorescence methods that require protein over-expression for detection.

"For the first time we saw native Cdc42 activity in living cells," Hahn said. "But perhaps the most important aspect of the paper is that we demonstrated a new approach: We showed we can look at endogenous molecules and their activation using novel dyes."

Unlike other protein visualization methods, "you're looking directly at the fluorescence from this dye, which means it's much brighter and more sensitive," Hahn said.

Also differing from current methods, the new approach does not require making modifications to the protein in question.

"Many proteins occur in small amounts, so if you put in exogenous material you change everything," Hahn said.
'"/>

Contact: L. H. Lang
llang@med.unc.edu
919-843-9687
University of North Carolina School of Medicine
10-Sep-2004


Page: 1 2

Related biology news :

1. MRI technique lets researchers directly compare monkey and human brains
2. Discovery sheds light on mechanisms that allow cells to steer directly toward chemical cues
3. Immune molecule found to kill indirectly by closing receptor to live-saving help
4. Hidden diversity: DNA barcoding reveals a common butterfly is actually 10 different species
5. Brown research reveals key insight into memory-making
6. Study reveals why eyes in some paintings seem to follow viewers
7. Study reveals first genetic step necessary for prostate cancer growth
8. Study of flu patients reveals virus outsmarting key drug
9. Study in Science reveals recreational fishing takes big bite of ocean catch
10. Plumbing trees plumbing reveals their engineering skill
11. New survey reveals insights into unique relationship between mothers and pediatricians

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... 2017 Research and Markets has announced the ... to their offering. ... eye tracking market to grow at a CAGR of 30.37% during ... Market 2017-2021, has been prepared based on an in-depth market analysis ... and its growth prospects over the coming years. The report also ...
(Date:4/11/2017)... Florida , April 11, 2017 ... a security technology company, announces the appointment of independent Directors ... Bendheim to its Board of Directors, furthering the company,s ... ... of NXT-ID, we look forward to their guidance and benefiting ...
(Date:4/5/2017)... -- The Allen Institute for Cell Science today announces the ... and dynamic digital window into the human cell. The ... of deep learning to create predictive models of cell ... growing suite of powerful tools. The Allen Cell Explorer ... available resources created and shared by the Allen Institute ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... ... October 10, 2017 , ... For the second ... a US2020 STEM Mentoring Award. Representatives of the FirstHand program travelled to Washington, ... from US2020. , US2020’s mission is to change the trajectory of STEM education ...
(Date:10/10/2017)... International research firm Parks Associates announced today that ... TMA 2017 Annual Meeting , October 11 in Scottsdale, ... security market and how smart safety and security products impact the competitive ... Parks Associates: Smart Home Devices: Main ... "The residential security market has experienced continued ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... and business process optimization firm for the life sciences and healthcare industries, announces ... conference in San Francisco. , The presentation, “Automating GxP Validation for Agile Cloud ...
(Date:10/9/2017)... , Oct. 9, 2017  BioTech Holdings announced ... by which its ProCell stem cell therapy prevents ... ischemia.  The Company, demonstrated that treatment with ProCell ... limbs saved as compared to standard bone marrow ... HGF resulted in reduction of therapeutic effect.  ...
Breaking Biology Technology:
Cached News: