HOME >> BIOLOGY >> NEWS
New method is first to mimic subtle genetic changes

A new research technique has proven to be a potent tool for predictably altering gene expression in mice without disrupting the regulatory elements that are essential for normal gene function. The research, published in the April Developmental Cell, may prove to be invaluable for future studies designed to unravel complex pathological changes in gene expression.

Many human diseases are related to changes in the level of gene expression, and therefore the amount of gene product produced, without any obvious alteration of the gene product itself or the timing of gene expression. However, these types of changes have been notoriously difficult to study in the laboratory because traditional methods for changing levels of gene expression tend to also disrupt multiple elements that are required for gene function, thereby making it nearly impossible to draw conclusions related solely to quantitative differences in gene expression. Dr. Oliver Smithies and colleagues from the University of North Carolina at Chapel Hill tried to overcome these problems by developing a totally new procedure that can be used to alter expression levels while retaining essential control elements.

The researchers altered a region of the gene, called the 3' untranslated region (UTR), that does not specifically control gene function but ultimately influences how much protein product the gene produces. As an initial test of the new method, the researchers used a gene for a protein that glows green as a reporter system. By using protein fluorescence as a quantitative indicator of gene expression, the researchers determined that modification of the 3' UTR changes expression of the gene in a predictable fashion in mice. These results were confirmed in different types of cells and by increasing or decreasing expression of functionally different native mouse genes. This technique may be very useful for construction of animal models that can be used to better understand complex human geneti
'"/>

Contact: Heidi Hardman
hhardman@cell.com
617-397-2879
Cell Press
12-Apr-2004


Page: 1 2

Related biology news :

1. OHSU researchers discover possible method for early detection, prevention of premature birth
2. Japanese researchers develop novel method of introducing transgenes into animals
3. New screening method turns up potential compound for treating anthrax
4. Radiologists provide safe delivery method for gene therapy
5. New method for determining age of artifacts fills chronological gap for scientists
6. Proven method of bone analysis may clarify human origins
7. UVa researchers describe method of protecting chromosomes during cell division
8. Computer method identifies potentially active enzymes
9. Risk and food are on the same plate: World food safety experts offer new methods to assess risk
10. Purdue engineers develop quick, inexpensive method to prototype microchips
11. Test method provides biocompatibility barometer

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/1/2018)... ... October 01, 2018 , ... Paragon Biosciences CEO Jeff Aronin was recently ... and for his work bringing life-altering drugs to patients in need. Jeff’s vision has led ... earned by the R&D teams now at Paragon and its portfolio companies. , ...
(Date:9/28/2018)... (PRWEB) , ... September 28, 2018 , ... ... DNA profiles previously thought to be too complex to interpret – “is scientifically ... the Virgin Islands. , Denying a defense motion for a Daubert hearing on ...
(Date:9/27/2018)... ... September 27, 2018 , ... ... knowledge encoded into synthetic DNA by Microsoft, Twist Bioscience Corporation, and the University ... feature 10,000 crowdsourced images and the full text of 20 important books, among ...
Breaking Biology News(10 mins):
(Date:9/28/2018)... ... September 28, 2018 , ... ... tech innovators, engineers, and scientists from around the world, is excited to announce ... of San Marcos, as the winner of the LabRoots Summer 2018 Textbook Scholarship, ...
(Date:9/27/2018)... ... September 27, 2018 , ... ... Everwell Health Solutions and SureAdhere Mobile Technology, Inc. announced they will formally merge ... in the battle against tuberculosis (TB) and other diseases. These two social enterprise ...
(Date:9/22/2018)... ... September 21, 2018 , ... ... and pathogenesis requires tools that can rapidly and consistently quantify the expression ... Imaging by Time-Of-Flight (MIBI-TOF) detects elements from hydrogen to uranium, permitting simultaneous ...
(Date:9/12/2018)... ... September 11, 2018 , ... Leading regenerative ... of Feline Practitioners Conference (AAFP) later this month. The company’s CEO ... therapy for the treatment of chronic kidney disease in felines at a poster ...
Breaking Biology Technology:
Cached News: