New papillomavirus target could lead to specific antiviral drugs for precancerous cervical lesions

RD4. Though little was known about it, a mouse homolog had been identified as a mitotic chromosome-associated protein. "It immediately jumped out at us that this could very well be the mitotic tether that we had been looking for," said Howley.

The researchers performed a series of experiments, each of which would confirm the hunch. To begin, they used immunofluorescence staining to see where E2 and BRD4 resided in infected mouse cells. Both localized to the mitotic chromosomes. In fact, biochemical tests showed that the proteins did interact. Next, they blocked BRD4 with a competitive mutant, or dominant negative, version of the protein. Sure enough, upon immunofluorescence staining, E2 no longer appeared to be associated with the mitotic chromosomes.

Most exciting, the researchers found that when the dominant negative BRD4 competitors were introduced into mouse cells turning cancerous by a strain of bovine papillomavirus, the cells' rate of transformation to cancer declined by over 90 percent. "All the evidence pointed to BRD4 as the receptor for E2," said You.

That is probably true of human papillomavirus as well. You and colleagues found that human papillomavirus E2 interacts with BRD4. In fact, the region on the E2 protein that binds BRD4 is highly conserved in all species of papillomavirus. "That suggests to us that BRD4 is probably the common target for all the papillomavirus E2 proteins," said Howley.

As it turns out, papillomaviruses are not the only viruses that have the task of maintaining and partitioning their viral genome in a dividing cell. Epstein-Barr and Kaposi's sarcoma herpesviruses face a similar challenge. "To date, none of the mitotic receptors for any of these other viruses has been found," said Howley. He and his colleagues are currently exploring the possibility that BRD4 is the mitotic tether for these viruses as well--and that drugs targeting BRD4 could be used to treat an even broader array of infections.


Page: 1 2 3 4

Related biology news :

1. Hopkins scientists link human papillomavirus (HPV) to head and neck cancer
2. International study findings link acne-like rash to effectiveness of new targeted cancer treatment
3. Safety of isoflavones in dietary supplements targeted by Illinois initiative
4. Cancer vaccine based on pathogenic listeria bacteria shows promise targeting metastases
5. Evidence builds for potential new cancer drug target
6. UCI scientists successfully target key HIV protein; breakthrough may lead to new drug therapies
7. Images of tail of protein needed for cell multiplication suggest anticancer drug targets
8. Diabodies act as guided missiles targeted to mammary tumor growth
9. Anticancer drug zebularine specifically targets tumor cells
10. Study suggests cell-cycle triggers might be cancer drug targets
11. Sweet success in targeting sugar molecules to cells in living animals

Post Your Comments:

(Date:5/28/2020)... ... May 27, 2020 , ... ... Workforce Advisor™ , a solution that uniquely combines lab testing insights and hc1’s ... systematically support the ongoing health and safety of employees returning to the workplace. ...
(Date:5/28/2020)... , ... May 28, 2020 , ... ... that interim results from their 1801 Phase 1/2 study (NCT03678883, EudraCT #: 2018-003739-32) ... Meeting which will take place virtually on May 29th, 2020. Dr. Benedito Carneiro, ...
(Date:5/25/2020)... ... May 22, 2020 , ... Oxford Nanopore Technologies is ... of SARS-CoV-2, the virus that causes COVID-19. , The LamPORE assay is ... smaller sample numbers, to very large numbers of samples. Hardware and reagents sit ...
Breaking Biology News(10 mins):
(Date:5/14/2020)... ... May 12, 2020 , ... DuPont Nutrition ... distribution partner for all personal care ingredients in Russia and Belarus. Beginning May ... preservative portfolio for personal care. , The GENENCARE®OSMS product line contains ...
(Date:5/9/2020)... ANGELES (PRWEB) , ... May 08, 2020 , ... ... episode of their successful business program on Sunday, May 10, 2020 and Wednesday, May ... U.S. coast! This episode’s exclusive interviews will spotlight key executives from Techolution ...
(Date:5/1/2020)... NEW YORK (PRWEB) , ... April 30, 2020 ... ... Ideas Awards were announced today, honoring the businesses, policies, projects, and concepts that ... is a revolutionary bioprinter for living cells, which has taken out the top ...
(Date:4/22/2020)... CHESTER, Ohio (PRWEB) , ... April 21, 2020 ... ... of Biophen™ FXIII chromogenic method for determination of FXIII. , Aniara Diagnostica, a ... strengthened its commitment to meeting today’s highest standards for product quality and cost-efficient ...
Breaking Biology Technology:
Cached News: