HOME >> BIOLOGY >> NEWS
New proteomics tool offers a clear look at cellular proteins IDAT can detect proteins at an unprecedented resolution for research and clinical use

(Philadelphia PA) Scientists may have identified the genes in the human genome, but proteomics is the growing field of research that describes how proteins encoded in those genes work. Researchers at the University of Pennsylvania School of Medicine have created the first new technology for the proteomic era, a technique sensitive enough to detect individual proteins and robust enough to screen hundreds or thousands of molecules in mass automation.

The versatile technique, called IDAT, has a variety of potential uses from detecting cancer earlier to sifting through samples of molecules to find new candidates for drug research. In todays edition of Proceedings of the National Academy of Science, the researchers describe how they used IDAT to identify a protein marker for breast cancer at a resolution up to nine orders of magnitude more powerful than conventional techniques, and explain how the technique can be further refined.

"Nine orders of magnitude is a significant jump. If we were discussing computers, we would be talking about the differences between bytes and Gigabytes," said Mark I. Greene, MD, PhD, Professor in the Penn Department of Pathology and Laboratory Medicine. "IDAT has the potential to do for proteomics what PCR did for genomics in the last two decades."

IDAT works by snagging a target protein even in a vast mixture of separate molecules and broadcasting the presence of the targeted protein with a strong signal. IDAT stands for Immuno-Detection Amplified by T7 RNA polymerase and it combines the detecting ability of antibodies, highly specific immune system molecules, with the speed of a particular enzyme, T7 RNA polymerase, to act as an alarm system. The enzyme and the antibody both interact through another molecule called a promoter. When the antibody snags the targeted protein, it triggers the promoter that, in turn, triggers the enzyme into creating the signal. Researchers could then screen for the signal, actually a molecule
'"/>

Contact: Greg Lester
lesterg@uphs.upenn.edu
215-349-5658
University of Pennsylvania School of Medicine
22-Apr-2001


Page: 1 2 3

Related biology news :

1. 16 APS exercise research highlights, from reduced flu mortality to proteomics & obesity
2. New proteomics core at OHSU will speed discovery process
3. Michigan receives NCRR grant for tomorrows proteomics technology
4. PNNL wins record $10.2 million NIH grant for proteomics center
5. Purdue instrument to fashion custom-made proteomics chips
6. Fighting Mycobacterium tuberculosis with structural proteomics
7. NHLBI launches innovative proteomics centers
8. Collaboration to use proteomics to unravel mysteries of heart disease
9. Mass spectrometer weighs in as proteomics breakthrough
10. UB scientists report fast, simple method of generating designer RNA catalysts for proteomics
11. CF Foundation seizes proteomics for drug discovery

Post Your Comments:
(Date:7/29/2014)... has received from the National Institutes of Health ... project to support the further development of 23andMe,s ... , Specifically, the grant supports four areas ... to improve the company,s ability to identify novel ... to support the collection of a broader set ...
(Date:7/29/2014)... 1 in 100,000 people characterized by a loss of ... unknown, a new study by a team of researchers at ... and other European institutions confirms for the first time that ... July in Nature Genetics , is an important step ... sphincter in the lower esophagus opens, allowing food to enter ...
(Date:7/28/2014)... of two additional coral communities showing signs of damage from ... the 2010 spill in the Gulf of Mexico. The discovery ... of biology at Penn State University. A paper describing this ... the Gulf of Mexico will be published during the last ... the journal Proceedings of the National Academy of Sciences ...
Breaking Biology News(10 mins):23andMe scientists receive approximately $1.4 million in funding from National Institutes of Health 223andMe scientists receive approximately $1.4 million in funding from National Institutes of Health 3Mysterious esophagus disease is autoimmune after all 2Impact of Deepwater Horizon oil spill on coral is deeper and broader than predicted 2
(Date:7/29/2014)... Second generation biofuels are a viable solution to ... as natural gas is very cheap, this is affecting the ... valuable as they could be in comparison to natural gas. ... made it difficult for renewable energy developers to get the ... For these reasons, certain companies have shifted their focused within ...
(Date:7/29/2014)... , July 29, 2014  Cannabis Science, Inc. ... formulation-based drug development and related consulting, applauds the ... taking a strong position in opposition to current ... 27, 2014 publication.  The New York Times also ... this week exploring the issue called "High Time:  ...
(Date:7/29/2014)... Tenn. (PRWEB) July 29, 2014 ... in biomass feedstock supply innovation and supply chain ... proprietary feedstock management systems, Energy Grangeā„¢ and Supply ... of R&D and in-the-field testing aimed at consolidating ... offering benefits to landowners, farmers and feedstock end ...
(Date:7/29/2014)... 29, 2014 G-CON Manufacturing Inc., the ... today announced a new leadership appointment designed to drive ... and abroad. , Maik Jornitz, Chief Operating Officer ... President charged with leading the pioneering company through its ... this stage of development and look forward to providing ...
Breaking Biology Technology:Renewable Waste Intelligence: Could Renewable Chemicals Be the Future for the Renewable Energy Industry? 2Cannabis Science, Inc. (CBIS) Applauds New York Times Editorial Board Stance on 'Repealing Prohibition, Again'; NYT Editorial Is Bell Weather of Change 2Cannabis Science, Inc. (CBIS) Applauds New York Times Editorial Board Stance on 'Repealing Prohibition, Again'; NYT Editorial Is Bell Weather of Change 3Genera Energy Introduces Proprietary Feedstock Management Systems 2Genera Energy Introduces Proprietary Feedstock Management Systems 3Genera Energy Introduces Proprietary Feedstock Management Systems 4G-CON Manufacturing, Inc. Announces Maik Jornitz as President 2
Cached News: