HOME >> BIOLOGY >> NEWS
New toxicity test could cut animal testing

To test whether chemicals are toxic to humans, researchers need to use liver cells that have been freshly harvested from mice or other mammals. A new collection of stable cell lines, described in BMC Biotechnology this week, could reduce the numbers of animals needed in such experiments.

The MMH-GH cell lines are derived from the liver cells of transgenic mice. These cells have been engineered to secrete human growth hormone when they are exposed to toxic compounds. The cells also continuously produce an activated version of the growth factor receptor, c-MET, which enables them to survive for longer than normal liver cells and to retain features of differentiated liver cells when they are grown in culture.

The researchers who created the cell lines come from Istituto Tecnologie Biomediche-National Research Council in Milan and Universit La Sapienza in Rome. They write: "We believe that the MMH-GH cell lines provide a cheap, reproducible, rapid, reliable and ethically acceptable tool," for assessing the toxicity of chemicals.

To test their system the researchers added toxic arsenic and cadmium compounds to the cells and then looked for human growth hormone in the culture media. They found that even at low doses these compounds caused the cells to secrete the hormone and were therefore deemed to be toxic. These low concentrations of the chemicals would not have been picked up by current toxicity-testing methods, which brand a chemical as 'toxic' only if it kills liver cells.

As the MMH-GH cell lines can survive for longer than cultured primary liver cells, the cell type most commonly used in toxicity testing, adopting this new technique would reduce the number of animals used in such experiments. Currently researchers must repeatedly derive fresh primary cells from animals or humans, which is time consuming, costly, and ethically undesirable.

The researchers believe that experiments using the transgenic cell lines will also b
'"/>

Contact: Gemma Bradley
press@biomedcentral.com
44-207-323-0323
BioMed Central
18-Mar-2004


Page: 1 2

Related biology news :

1. Rice finds on-off switch for buckyball toxicity
2. Stop leak solution found for toxicity problems in experimental gene therapy
3. Scientists uncover early warning system for copper toxicity
4. Strategy to treat AIDS patients in Africa with less drug, reduced toxicity set for clinical test
5. Mouse study identifies protective mechanism against alcohol-induced embryo toxicity
6. Researchers combine electronics with living cell to create potential toxicity sensor
7. Study of insecticide neurotoxicity yields clues to onset of Parkinsons Disease
8. New findings reconfirm toxicity of Pfiesteria cultures
9. Cadmium toxicity threatening wildlife in Rocky Mountains
10. Research zeroes in on killer molecule in dioxin toxicity
11. Harvard expert speaks on lead toxicity to chemists meeting in New Orleans

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/6/2018)... ... December 06, 2018 , ... Stewart has been on ... services, throughout his career. During his recent five-year tenure with InSphero he was ... the commercial laboratory operations in Brunswick, Maine. Through these experiences, Stewart has a ...
(Date:12/5/2018)... ... December 04, 2018 , ... Slone ... announces the placement of Michael Mercer at Karius, Inc. as Chief Commercial Officer. ... will be based at Karius' headquarters in Redwood City, California and report to ...
(Date:11/29/2018)... Calif. (PRWEB) , ... November 29, 2018 , ... ... Technologies, is excited to collaborate with the new NSF Engineering Research Center for ... and clinical collaborators brought together to develop transformative tools and technologies for the ...
Breaking Biology News(10 mins):
(Date:11/29/2018)... LEHI, Utah (PRWEB) , ... November 29, 2018 , ... ... round was led by Kickstart Seed Fund, with participation from existing investor and board ... Investors and Davis Smith of Cotopaxi. The additional funding will enable the company to ...
(Date:11/20/2018)... Columbia (PRWEB) , ... November 19, 2018 , ... ... is pleased to announce the release of the Chinese translation of Best Practices: ... January 2018, is a practice-changing publication aimed at advancing the science of biobanking ...
(Date:11/15/2018)... ... 2018 , ... Hüpnos announces the launch of ... It is estimated that 90 million Americans suffer from persistent snoring, ... resulting daytime fatigue causes extreme difficulty with concentration, focus and memory. It’s ...
(Date:11/13/2018)... ... November 13, 2018 , ... Aditya Humad, Managing Partner of ... that asserts it is still trending upward with growing valuations based on a ... market consolidation, pricing pressure and increased competition as reasons often cited by institutional ...
Breaking Biology Technology:
Cached News: