News from the zebrafish cousin of the human genome: A radiation hybrid map of the zebrafish genome

A map of the zebrafish genome based on the radiation hybrid technique has been published by scientists from the Max Planck Institute for Developmental Biology in Tuebingen/Germany (Nature Genetics, September 1999). This map allows scientists to match the map positions of candidate genes with the positions of genetically mapped mutations, and finally sequence the mutations.

In recent years large-scale screens for mutant zebrafish have been conducted in the laboratories of Christiane Nsslein-Volhard in Tuebingen and Wolfgang Driever in Boston, yielding hundreds of mutations that can potentially provide insight into human development. These mutations give the zebrafish a unique advantage over other model organisms used in the human genome project, such as the mouse and rat. However, they must be analyzed on the DNA sequence level to be useful. Scientists at the Max Planck Institute for Developmental Biology in Tuebingen have now published a map of the zebrafish genome based on the radiation hybrid technique. With this map in hand, researchers will be able to match the map positions of candidate genes with the positions of genetically mapped mutations, and finally sequence the mutations.

The foundation for the map was laid by the lab of Peter Goodfellow (London), who fused irradiated zebrafish cells with rodent cells, producing a panel of hybrid cell lines each of which contains a large number of random fragments of the zebrafish DNA. To map a known piece of DNA (referred to as a marker), each of the cell lines must be tested for its presence or absence using the polymerase chain reaction (PCR) technique. Whenever two markers are retained in many of the same cell lines, they are placed next to each other on the map, until a map of the whole genome emerges.

Throughout the summer of 1998, more than thirty researchers from the departments of Christiane Nsslein-Volhard and Friedrich Bonhoeffer worked long hours on the zebrafish map. 1275 markers were p

Contact: Robert Geisler

Page: 1 2

Related biology news :

1. $2.7 million grant to support creation of virtual zebrafish atlas
2. Learned social preference in zebrafish
3. Major boost for European zebrafish research
4. Modified zebrafish may point the way toward T cell leukemia genes, aid in drug development
5. Childrens Hospital Boston researchers regenerate zebrafish heart muscle
6. A highly conserved mechanism used during the development of zebrafish and fruitfly eyes points to a common evolutionary origin of animal eyes
7. Manatees cousin faces extinction on Tanzanian coast
8. Hatchery salmon may endanger wild cousins
9. Gene researchers close in on nicotines evil cousin
10. Understanding our ape cousins: chimpanzee genetics may shed light on human diseases
11. Climate change plus human pressure caused large mammal extinctions in late Pleistocene

Post Your Comments:

(Date:4/22/2020)... ... ... RCH Solutions (RCH ), a global provider of Bio-IT computing expertise for ... as Director of Sales and Business Development. Based out of RCH’s location in Boston, ... across the Northeast. , “We continue to see increased demand for specialized scientific computing ...
(Date:4/22/2020)... , ... April 21, 2020 , ... ... transformation and innovation in technology and compliance, announces a new managed service to ... trio of alliances – Box, ComplianceQuest, and USDM Life Sciences (USDM) – Unify ...
(Date:4/18/2020)... ... April 16, 2020 , ... SIRION Biotech GmbH , ... today announced it will present a company update on its work expediting gene therapy ... taking place on April 22 and 23. Dieter Lingelbach, the Company’s Chief Operating Officer, ...
Breaking Biology News(10 mins):
(Date:5/21/2020)... ... May 21, 2020 , ... Threats to intellectual property, ... , Contrary to conventional wisdom that says the coronavirus pandemic will generally benefit ... emerge from the pandemic commercially weaker, dealing with delays in new product launches ...
(Date:5/15/2020)... ... May 14, 2020 , ... ... working cell banks and hMSC bioprocess systems, today announced the launch of ... hMSC-derived extracellular vesicles (EVs). RoosterCollect™-EV-CC is the only system designed with hMSC-EV ...
(Date:5/14/2020)... ... May 12, 2020 , ... ... the new distribution partner for all personal care ingredients in Russia and Belarus. ... DuPont preservative portfolio for personal care. , The GENENCARE®OSMS product line ...
(Date:5/9/2020)... ANGELES (PRWEB) , ... May 08, 2020 , ... ... episode of their successful business program on Sunday, May 10, 2020 and Wednesday, May ... U.S. coast! This episode’s exclusive interviews will spotlight key executives from Techolution ...
Breaking Biology Technology:
Cached News: