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Nitric Oxide Found To Control Cells By Turning On Genes; Suggests New Role In Health And Disease

rning genes on and off to keep NO in equilibrium. If this equilibrium is upset, Stamler theorizes, it may lead to a number of diseases that have recently been associated with NO, including cancer, stroke, hardening of heart arteries, infectious disease, and arthritis.

The scientists say that an immediate application from the research may be a new way to disarm invasive bacteria that have become resistant to antibiotics -- a major problem in the treatment of infections, especially in hospital settings.

Other contributors to the work include Alfred Hausladen, a Duke biochemist, and Christopher Privalle and Teresa Keng, two former Duke researchers who are now scientists at Apex Bioscience Inc., Research Triangle Park, N.C. Joseph DeAngelo, director of research at Apex, also collaborated on the project.

This is the second recent discovery Stamler has made in NO research. Earlier this year, he found that an NO compound, combined with hemoglobin, is a major regulator of gas exchange, as well as blood pressure, in the circulatory system. That work was published March 21 in the British journal Nature.

The current discovery was made when the researchers were studying why some bacteria escape attack by immune system cells called macrophages, which are designed to disable and devour them. The question is of increasing importance in acute health care.

When bacteria invade the body, the immune system floods the bacterial cell with noxious oxygen and nitrogen-free radicals.

The researchers found that when NO enters a bacterium, the NO attaches to a particular place on proteins called a thiol, a protein subunit that contains sulfur. The NO-thiol coupling forms a new NO-compound called SNO (for S-nitrosothiol). SNOs are souped-up cousins of NO molecules, and they have a wider range of functions.

To defend themselves, bacteria deploy an army of small thiol
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Contact: Karyn Hede George, Renee Twombly
george016@mc.duke.edu
919-684-8066
Duke University
6-Sep-1996


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