New York, N.Y., September 25, 2004 Mesothelioma is an aggressive cancer of the chest cavity that kills about 2000 people a year in the United States. Seventy to eighty percent of patients with this rare cancer have had exposure to asbestos. It has also been proposed that simian virus 40 (SV40), a contaminant in some polio vaccines administered in the 1950's and 1960's, might be a cause. However, studies reporting the detection of SV40 DNA in human tumors (including mesotheliomas, and also some lymphomas, brain cancers, and bone cancers) have not consistently yielded the same results when repeated by other groups. This has fueled an ongoing debate over laboratory methods and the strength of the association of SV40 with these tumors.
A study, published in the September 25 issue of Lancet, calls into question this proposed link between SV40 and pleural mesothelioma and provides a possible explanation for the discrepancies in the results obtained by different groups. Researchers at Memorial Sloan-Kettering Cancer Center (MSKCC) used several independent methods to detect SV40 DNA, SV40 RNA, and SV40 proteins in human pleural mesothelioma samples and found no evidence for a significant role for SV40 in human mesotheliomas. Unexpectedly, they found that the SV40 DNA fragments detected in some assays were not derived from genuine SV40 in the tissue samples but from SV40 DNA fragments engineered into common laboratory plasmid vectors used in molecular biology research. This source of SV40 DNA fragments may be unrecognized, leading to misinterpretation of assay results as indicating the presence of genuine SV40 in human tumors. Their findings are a caution to other researchers to be vigilant in avoiding these technical errors when planning future studies with SV40.
"Because SV40 was a recognized contaminant of some polio and adenovirus vaccines in the 50's and early 60's, its potential to cause cancer in humans has been a source of intense debate since Page: 1 2 Related biology news :1
Contact: Joanne Nicholas
Memorial Sloan-Kettering Cancer Center
. Pacific Northwest team unveils largest virus proteome to date2
. Cannabis may help combat cancer-causing herpes viruses3
. Scientists find nanowires capable of detecting individual viruses4
. $7.5 Million grant to Yale researchers for role of viruses in cancer5
. Simian virus 40 not associated with non-Hodgkin lymphoma, study shows6
. Common cold virus can cause polio in mice when injected into muscles7
. Defensive action by influenza viruses demonstrated by Hebrew university researcher8
. Study of flu patients reveals virus outsmarting key drug9
. Images reveal how rotavirus - leading cause of diarrhea - enters cells10
. Bright idea could doom cancer and viruses, say Purdue scientists11
. U-M scientists find common virus in human prostate tissue