ORNL Microscope "Sees" Bumps That Reveal Partial DNA Structure

OAK RIDGE, Tenn., Jan. 15, 1997 --Imagine looking down at a yellow carpet and seeing a long, brown, sinuous thread with an occasional knot. That's the picture scientists at the Department of Energy's (DOE) Oak Ridge National Laboratory (ORNL) get on a computer screen when using an atomic force microscope (AFM) to image a serpentine strand of DNA.

And when they get the picture, they get excited about the "knots." The reason: each knot is really a "protein bump" formed when a protein recognizes and attaches to a specific site on a DNA molecule.

These ORNL scientists are developing this new imaging approach as an alternative technology for DNA mapping. They think their technique will be more accurate and perhaps faster than conventional methods.

DNA, sometimes referred to as the blueprint for life, is a nucleic acid that carries the genetic information in the cell. It consists of two long chains of deoxyribose sugar molecules hooked together by phosphate groups and twisted into a double helix, not unlike a spiral staircase.

The work was done by Dave Allison and Mitch Doktycz, both of ORNL's Molecular Imaging Group, which is headed by Bruce Warmack. The group is in the Health Sciences Research Division.

The AFM is a microscope capable of atomic resolution that produces a three-dimensional image by tracing the surface of a material, similar to the arm and needle of a phonograph playing a record.

The research, which was performed for the Human Genome Project, was funded by the Department of Energy's Office of Health and Environmental Research. Continued research on the binding of transcription factors is being supported by ORNL's Laboratory Directed Research and Development Program.

ORNL, one of the Department of Energy's multiprogram national research and development facilities, is managed by Lockheed Martin Energy Research Corp.


Contact: Carolyn Krause
DOE/Oak Ridge National Laboratory

Page: 1

Related biology news :

1. Microscopes provide new view for tissue engineering
2. Laser Microscope At Cornell Images Serotonin In Live Cells
3. Revealing bizarre deep-sea secrets
4. Revealed: How a master protein remodels chromosomes to orchestrate gene expression
5. Revealing the complex patterns of cardiac disease
6. Study Reveals Cancer Role Of Mutated Gene
7. The Origins Of Hereditary Breast Cancer -- Studies With Knock-Out Mice Reveal Underlying Mechanisms
8. Finding Reveals Broader Role Of Telomerase Enzyme, Suggesting New Target For Extending Life Span Of Cell
9. Structure Of Fat-Depleting Protein Reveals Important Functional Clues
10. Research Reveals Environmental Injustice In N.C. Communities With Large Hog Farms
11. UI Study Reveals Important Information About Molecular Defect In Limb Girdle Muscular Dystrophy

Post Your Comments:

(Date:7/10/2020)... ... July 08, 2020 , ... Overcoming ... Therapy Regulation, An FDAnews Webinar, Wednesday, July 22, 2020 • 1:30 p.m.-3:00 p.m. ... what is the most effective way to complete one? Will the study comply ...
(Date:7/2/2020)... , ... July 01, 2020 , ... ... its phase 1a findings of Neihulizumab, a biologic for the treatment of steroid-refractory ... 2020. Led by hemato-oncologist Dr. Paul J Martin of the Fred Hutchinson Cancer ...
(Date:7/1/2020)... (PRWEB) , ... July 01, 2020 , ... ... building awareness and solutions for glioblastoma—the most common and aggressive adult brain cancer—announced ... Haile, JD, PhD. Senior Fellows are charged with supporting the organization’s initiatives and ...
Breaking Biology News(10 mins):
(Date:7/31/2020)... ... 29, 2020 , ... Diversified Technologies, Inc. has introduced a ... to drive Klystrons, TWTs, IOTs, and magnetrons. , DTI Radar Transmitter Systems ... a push-pull configuration; yielding fast fall time for a capacitive load. These all ...
(Date:7/31/2020)... ... July 29, 2020 , ... The SDX® ... in 16 countries, has reached its 20th anniversary of worldwide use. Introduced in ... at top universities including University of Pennsylvania, University of Michigan, University of Maryland, ...
(Date:7/18/2020)... ... July 16, 2020 , ... “We are thrilled to deliver this new technology ... technology of its kind on the market and we were pleased that the IFT ... of traditional cultured ingredients, creating a natural way to extend the shelf life and ...
(Date:7/10/2020)... , ... July 09, 2020 ... ... company, announced today that Massachusetts Institute of Technology (MIT) has expanded the ... license allows PathSensors to move into the point-of-care diagnostic market, focusing initially ...
Breaking Biology Technology:
Cached News: