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Ohio State genome map

e "transcriptional unit" Yuan refers to is a length of DNA that shows strong evidence of being a gene but which still requires future verification.

"Some researchers are unsettled by the certainty with which the Human Genome Consortium is presenting its lower gene count," said Fred Wright, assistant professor of human cancer genetics and lead author of the paper.

"In my view, the final number of genes-when it is known-will lie somewhere between their high of 40,000 and our value of 70,000."

The Ohio State map would have taken far longer to assemble without the help supercomputers at the Ohio Supercomputing Center. The work required four full weeks of supercomputing time. "Without that capability, the task would probably have taken at least a year," said Yuan.

"The computations involved millions of DNA sequences and were extremely time-consuming," said Wright. "One of the databases had over 2 million sequences, each of which had to be searched against the entire 2.8 billion base pairs in the genome draft. "Figuring out where those 2 million sequences belonged was, by itself, a major computational task."

The Ohio State map also contains revealing information about tissue-specific genes, genes that are active in some tissues but not in others.

"This has important implications for biology and for disease mapping," said Wright. "Genes that are
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Contact: Bo Yuan
Yuan.33@osu.edu
614-292-0656
Ohio State University
3-Jul-2001


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