HOME >> BIOLOGY >> NEWS
Powerful new method helps reveal genetic basis of cancer

In 1981, using a gene transfer method he pioneered, Wigler, working at Cold Spring Harbor Laboratory, discovered one of the first human oncogenes (the H-ras oncogene). Hyperactive or excess Ras protein, or alterations in cell functions related to Ras, are implicated in the majority of cancers. Today, cancer therapies that target components of the Ras pathway (e.g. farnesyltransferase inhibitors) are in clinical trials.

In 1993, Wigler and Nikolai Lisitsyn developed a powerful and versatile gene discovery method called Representational Difference Analysis (RDA). RDA enables researchers to "clone the differences" between any two sets of DNA (for example, normal versus tumor DNA isolated from the same patient, or uninfected cells versus cells infected with an unknown virus).

Since 1993, Wigler's lab, alone and in collaboration with groups at Columbia University and Tularik, Inc., has used RDA to identify several previously unrecognized oncogenes and tumor suppressors genes, primarily from sporadic breast cancers (the most common form of the disease). These include PTEN and DBC2 (tumor suppressor genes frequently missing from or inactive in a large proportion of breast and other cancers) in addition to KCNK9 (a gene whose identification revealed a previously unrecognized mechanism for oncogene action as well as an attractive therapeutic target, namely, potassium channels).

The new method, ROMA, is essentially "RDA on a chip." Combining RDA and DNA microarray analysis with the third "pillar" of the method--i.e. knowledge of the complete human genome sequence--gives ROMA unprecedented ability to detect copy number fluctuations on a genome-wide scale.


'"/>

Contact: Peter Sherwood
sherwood@cshl.edu
516-367-6947
Cold Spring Harbor Laboratory
15-Sep-2003


Page: 1 2

Related biology news :

1. Powerful machines are coming in small packages
2. Powerful technologies probe innate immunity
3. Powerful Anti-AIDS Agent Found In Tears And In Urine Of Pregnant Women
4. Team Solves X-ray Structure Of Powerful Enzyme
5. Synthetic Vitamin E And Natural Vitamin E Are Equally Powerful Antioxidents
6. OHSU researchers discover possible method for early detection, prevention of premature birth
7. Japanese researchers develop novel method of introducing transgenes into animals
8. New screening method turns up potential compound for treating anthrax
9. Radiologists provide safe delivery method for gene therapy
10. New method is first to mimic subtle genetic changes
11. New method for determining age of artifacts fills chronological gap for scientists

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/13/2019)... ... 2019 , ... KICVentures announced today its plans to host an ... Management-Driven ASC Conference in Chicago on Thursday, June 13th at 6:30 pm. The event ... in the 3rd floor Ticino Hospitality room in space #74T. , The event will ...
(Date:6/6/2019)... ... 05, 2019 , ... Pelican BioThermal , the global ... service center in Mexico City, Mexico. Mexico is the second largest pharmaceutical market ... $1.75 billion in pharmaceutical exports since 2015. The Mexico City network station will ...
(Date:6/4/2019)... ... June 04, 2019 , ... Most preclinical EEG ... seizure risk signals, usually based on variations of amplitude, frequency or spike train ... diagnostic methodology due to the complexity and variability of the signal, and automation ...
Breaking Biology News(10 mins):
(Date:7/9/2019)... Ky. (PRWEB) , ... July 08, 2019 , ... ... Award winner. The company’s SmartLight Indicator was recognized as the 2019 Innovative Product ... sensors industry forward. The prestigious awards were during Sensors Expo & Conference ...
(Date:6/12/2019)... TOWNSHIP, N.J. (PRWEB) , ... June 12, 2019 ... ... of custom built, helium-based leak testing instruments for the Pharmaceutical, Biotechnology, Medical Device ... with Shanghai Zillion has been signed. The agreement will grant exclusive rights for ...
(Date:6/11/2019)... Calif. (PRWEB) , ... June 11, 2019 , ... ... company of Personalized Stem Cells Inc. (PSC) , has announced that their ... Public Health, Food and Drug Branch for manufacturing. This is an important ...
(Date:5/31/2019)... ... 30, 2019 , ... World Compliance Seminars today announced a ... in Boston, MA. This peer recommended interactive workshop is always selected by professionals ... kick off with a compendial treatment of Data Integrity fundamentals. After laying the ...
Breaking Biology Technology:
Cached News: